Nanocrystalline diamond: <i>In vitro</i> biocompatibility assessment by MG63 and human bone marrow cells cultures

Amaral, M.; Dias, A.G.; Gomes, Pedro S.; Lopes, M. A.; Silva, Rui; Santos, J. D.; Fernandes, Maria Helena

doi:10.1002/jbm.a.31742

Cited by 124 publications

(91 citation statements)

References 44 publications

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“…As we could see from the result of the attachment and proliferation of MG63, the hydrophobic surface like NCD-o had dramatically promoting effect. Moreover the percentage of cells in S phase of mitosis on NCD-o surface significantly higher than other groups, the results correspond with previous findings 19) . The titanium surface modified with NCD -o not only had an obvious effect on cell proliferation, but also could promote the distribution to the osteoblast differentiation 20) , and the subsequent experiments would be continuing.…”

Section: Surface Characteristics Of Ncdsupporting

confidence: 92%

Characteristics of Nanocrystalline Diamond Films on Titanium Surface and its Impact on the Proliferation and Adhesion of Cells

Liu

Luo

et al. 2016

J. Hard Tissue Biology.

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Nanocrystalline diamond has extensive application in biology modified material aspects of human implants, because it has good mechanical properties such as corrosion resistance and biocompatibility. This study investigated the surface characteristics and biocompatibility of pure titanium whose surface was modified with nanocrystalline diamond by Microwave Plasma Chemical Vapor Deposition. Scanning electron microscope (SEM), Spectruman analysis ray diffraction and X-Ray photoelectron spectroscopy were used to analyze the chemical composition and surface topography of nanocrystalline diamond. We found that the particle size of the diamond on the titanium disc is nanocrystalline, and is without impure elements. In the experiment, we used Human Osteoblast-like cells MG63 to investigate the bioactivity of nanocrystalline diamond film. The attachment and proliferation of the cells were tested by NAPI, CCK-8. The result showed that oxygen terminated NCD groups had a significant advantage compared with hydrogen terminated and titanium groups on attachment and proliferation of MG63.

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Section: Surface Characteristics Of Ncdsupporting

confidence: 92%

Characteristics of Nanocrystalline Diamond Films on Titanium Surface and its Impact on the Proliferation and Adhesion of Cells

Liu

Luo

et al. 2016

J. Hard Tissue Biology.

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“…The B-NCD thin films have been subject to a great deal of attention due to their outstanding properties, remarkable hardness, optical transparency in a broad wavelength [31,32], high thermal conductivity [33] as well as biocompatibility [34,35]. Unlike the rough polycrystalline films with grain sizes above 500 nm, NCD films with typical grain sizes and surface roughness of less than 100 nm are relatively smooth showing properties close to single crystal diamond [36].…”

Section: Introductionmentioning

confidence: 99%

Fabrication and characterization of boron-doped nanocrystalline diamond-coated MEMS probes

et al. 2016

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Fabrication processes of thin boron-doped nanocrystalline diamond (B-NCD) films on silicon-based micro-and nano-electromechanical structures have been investigated. B-NCD films were deposited using microwave plasma assisted chemical vapour deposition method. The variation in B-NCD morphology, structure and optical parameters was particularly investigated. The use of truncated cone-shaped substrate holder enabled to grow thin fully encapsulated nanocrystalline diamond film with a thickness of approx. 60 nm and RMS roughness of 17 nm. Raman spectra present the typical boron-doped nanocrystalline diamond line recorded at 1148 cm -1 . Moreover, the change in mechanical parameters of silicon cantilevers over-coated with boron-doped diamond films was investigated with laser vibrometer. The increase of resonance to frequency of over-coated cantilever is attributed to the change in spring constant caused by B-NCD coating. Topography and electrical parameters of boron-doped diamond films were investigated by tapping mode AFM and electrical mode of AFM-Kelvin probe force microscopy (KPFM). The crystallite-grain size was recorded at 153 and 238 nm for boron-doped film and undoped, respectively. Based on the contact potential difference data from the KPFM measurements, the work function of diamond layers was estimated. For the undoped diamond films, average CPD of 650 mV and for borondoped layer 155 mV were achieved. Based on CPD values, the values of work functions were calculated as 4.65 and 5.15 eV for doped and undoped diamond film, respectively. Boron doping increases the carrier density and the conductivity of the material and, consequently, the Fermi level.

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“…In addition, diamond also exhibits superior electronic properties (including high carrier mobility), large electrochemical potential window, low dielectric constant, controllable surface termination, and a high breakdown voltage. Furthermore, when considering the well-known combination of chemical inertness and high degree of biocompatibility [1], diamond became recently a promising candidate for applications like artificial photosynthetic water-splitting, where interfaces between a photo-electrode surface and a redox protein is of utterly importance [2].…”

Section: Introductionmentioning

confidence: 99%

Effect of CVD diamond growth by doping with nitrogen

Yi-Ming

Larsson

2013

Theor Chem Acc

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The purpose with the present investigation has been to support and explain the experimental observation made regarding the enhancing effect by N doping on especially the diamond (100)-2 9 1 growth rate. Within the present study, also the commonly observed diamond (111) and (110) surfaces were included, all assumed to be H-terminated. Density functional theory calculations were used, based on a plane wave approach under periodic boundary conditions. It was shown that the surface H abstraction reaction is most probably the rate-limiting step during diamond growth. In addition, the results showed that it is N, substitutionally positioned within the upper diamond surface, that will cause the growth rate improvement, and not nitrogen chemisorbed onto the growing surface in the form of either NH (or NH 2 ). The here presented numerical value for the growth rate enhancement for the diamond (100)-2 9 1 surface is almost identical with the experimentally obtained one (3.7 vs. 3.6). In addition, the (111) and (110) surfaces were shown to undergo a different growth rate enhancement, with about half as much for the (111) and (110) surfaces as compared to the diamond (100)-2 9 1 surface (1.9, 1.7 vs. 3.7). Despite the rate improvement for all surface planes, this difference will bring about a preferred diamond (100) surface texture.

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Nanocrystalline diamond: In vitro biocompatibility assessment by MG63 and human bone marrow cells cultures

Cited by 124 publications

References 44 publications

Characteristics of Nanocrystalline Diamond Films on Titanium Surface and its Impact on the Proliferation and Adhesion of Cells

Characteristics of Nanocrystalline Diamond Films on Titanium Surface and its Impact on the Proliferation and Adhesion of Cells

Fabrication and characterization of boron-doped nanocrystalline diamond-coated MEMS probes

Effect of CVD diamond growth by doping with nitrogen

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