Nanofibrous composite scaffolds of poly(ester amides) with tunable physicochemical and degradation properties

Mukundan, Shilpaa; Sant, Vinayak; Goenka, Sumit; Franks, Johnathan; Rohan, Lisa C.; Sant, Shilpa

doi:10.1016/j.eurpolymj.2015.04.026

Cited by 23 publications

(16 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2C). AlamarBlue Ò assay measures ability of metabolically active cells to reduce resazurin to fluorescent resorufin [27]. All scaffolds supported growth of C2C12 as evident from the significant increase in their metabolic activity over a period of 7 days.…”

Section: Effect Of Nano-roughness and Wettability On Myoblast Adhesiomentioning

confidence: 99%

See 1 more Smart Citation

Carbon-based hierarchical scaffolds for myoblast differentiation: Synergy between nano-functionalization and alignment

et al. 2016

Self Cite

View full text Add to dashboard Cite

Due to limited regenerative potential of skeletal muscle, strategies stimulating regeneration of functional muscles are important. These strategies are aimed at promoting differentiation of progenitor cells (myoblasts) into multinucleated myotubes, a key initial step in functional muscle regeneration. Recent tissue engineering approaches utilize various scaffolds ranging from decellularized matrices to aligned biomaterial scaffolds. Although, majority of them have focused on nano- or microscale organization, a systematic approach to build the multiscale hierarchy into these scaffolds is lacking. Here, we engineered multiscale hierarchy into carbon-based materials and demonstrated that the nanoscale features govern the differentiation of individual myoblasts into myocytes whereas microscale alignment cues orchestrate fusion of multiple myocytes into multinucleated myotubes underlining the importance of multiscale hierarchy in enhancing coordinated tissue regeneration.

show abstract

Section: Effect Of Nano-roughness and Wettability On Myoblast Adhesiomentioning

confidence: 99%

“…Surface characterization of as-prepared and cell-seeded Foam and Fiber scaffolds was carried out using SEM (JEOL 9335 Field Emission SEM) [27]. Cell-seeded scaffolds were fixed following 21 days and 14 days of culture for Foams and Fibers, respectively.…”

Section: Scanning Electron Microscopy (Sem)mentioning

confidence: 99%

Carbon-based hierarchical scaffolds for myoblast differentiation: Synergy between nano-functionalization and alignment

et al. 2016

Self Cite

View full text Add to dashboard Cite

show abstract

“…Poly(ester amide)s (PEAs) have emerged in recent years as an important family of biodegradable and bioresorbable synthetic polymers. Currently, several papers in the literature describe the synthesis, characterization, degradation and biomedical applications of biodegradable PEAs . HPLC/ESI‐TOF/MS was successfully used to monitor the enzyme‐mediated degradation of degradable multiblock PEAs based on natural amino acids, such as lysine and leucine, for controlled drug‐delivery applications.…”

Section: Bioresorbable Polymer Degradation Studies By Msmentioning

confidence: 99%

Mass spectrometry in bioresorbable polymer development, degradation and drug‐release tracking

Rizzarelli

Rapisarda

Valenti

2020

Rapid Comm Mass Spectrometry

View full text Add to dashboard Cite

A detailed characterization of polymeric matrices and appropriate degradation monitoring techniques are required to sustain the development of new materials as well as to enlarge the applications of the old ones. In fact, polymer analysis is essential for the clarification of the intrinsic relationship between structure and properties that ascertains the industrial applications in diverse fields. In bioresorbable and biodegradable polymers, the role of analytical methods is dual since it is pointed both at the polymeric matrices and at degradation tracking. The structural architectures, the mechanical and morphological properties, and the degradation rate, are of outstanding importance for a specific application. In some cases, the complexity of the polymer structure, the processes of decomposition or the low concentration of the degradation products need the concurrent use of different complementary analytical techniques to give detailed information of the reactions taking place. Several analytical methods are used in bioresorbable polymer development and degradation tracking. Among them, mass spectrometry (MS) plays an essential role and it is used to refine polymer syntheses, for its high sensitivity, to highlight degradation mechanism by detecting compounds present in trace amounts, or to track the degradation product profile and to study drug release. In fact, elucidation of reaction mechanisms and polymer structure, attesting to the purity and detecting defects as well as residual catalysts, in biodegradable and bioresorbable polymers, requires sensitive analytical characterization methods that are essential in providing an assurance of safety, efficacy and quality. This review aims to provide an overview of the MS strategies used to support research and development of resorbable polymers as well as to investigate their degradation mechanisms. It is focused on the most significant studies concerning synthetic bioresorbable matrices (polylactide, polyglycolide and their copolymers, polyhydroxybutyrate, etc.), published in the last ten years.

show abstract

“…To fabricate fibrous scaffolds mimicking native ECM, electrospinning of different natural and synthetic polymers has been widely used . We previously synthesized a library of biodegradable elastomers, specifically, poly(1,3‐diamino‐2‐hydroxypropane‐co‐glycerol sebacate)‐co‐poly (ethylene glycol) (APS‐co‐PEG) and developed isotropic (randomly aligned fibers) fibrous scaffolds by electrospinning polymer blends of APS‐co‐PEG and polycaprolactone (PCL) .…”

Section: Introductionmentioning

confidence: 99%

“…19,20 To fabricate fibrous scaffolds mimicking native ECM, electrospinning of different natural and synthetic polymers has been widely used. [21][22][23] We previously synthesized a library of biodegradable elastomers, specifically, poly(1,3-diamino-2-hydroxypropane-co-glycerol sebacate)-co-poly (ethylene glycol) (APS-co-PEG) 24 and developed isotropic (randomly aligned fibers) fibrous scaffolds by electrospinning polymer blends of APS-co-PEG and polycaprolactone (PCL). 25 By changing the PEG amount (15, 25, or 40 mole %) in the backbone of APS-co-PEG elastomer, the uniaxial mechanical properties of these scaffolds can be readily modulated.…”

mentioning

confidence: 99%

Valve leaflet‐inspired elastomeric scaffolds with tunable and anisotropic mechanical properties

Xue

Ravishankar

Zeballos

et al. 2019

Polymers for Advanced Techs

Self Cite

View full text Add to dashboard Cite

Fibrous scaffolds, which can mimic the elastic and anisotropic mechanical properties of native tissues, hold great promise in recapitulating the native tissue microenvironment. We previously fabricated electrospun fibrous scaffolds made of hybrid synthetic elastomers (poly(1,3‐diamino‐2‐hydroxypropane‐co‐glycerol sebacate)‐co‐poly (ethylene glycol) (APS‐co‐PEG) and polycaprolactone (PCL)) to obtain uniaxial mechanical properties similar to those of human aortic valve leaflets. However, conventional electrospinning process often yields scaffolds with random alignment, which fails to recreate the anisotropic nature of most of the soft tissues such as native heart valves. Inspired by the structure of native valve leaflet, we designed a novel valve leaflet‐inspired ring‐shaped collector to modulate the electrospun fiber alignment and studied the effect of polymer formulation (PEG amount [mole %] in APS‐co‐PEG; ratio between APS‐co‐PEG and PCL; and total polymer concentration) in tuning the biaxial mechanical properties of the fibrous scaffolds. The fibrous scaffolds collected on the ring‐shaped collector displayed anisotropic biaxial mechanical properties, suggesting that their biaxial mechanical properties are closely associated with the fiber alignment in the scaffold. Additionally, the scaffold stiffness was easily tuned by changing the composition and concentration of the polymer blend. Human valvular interstitial cells (hVICs) cultured on these anisotropic scaffolds displayed aligned morphology as instructed by the fiber alignment. Overall, we generated a library of biologically relevant fibrous scaffolds with tunable mechanical properties, which will guide the cellular alignment.

show abstract

Nanofibrous composite scaffolds of poly(ester amides) with tunable physicochemical and degradation properties

Cited by 23 publications

References 59 publications

Carbon-based hierarchical scaffolds for myoblast differentiation: Synergy between nano-functionalization and alignment

Carbon-based hierarchical scaffolds for myoblast differentiation: Synergy between nano-functionalization and alignment

Mass spectrometry in bioresorbable polymer development, degradation and drug‐release tracking

Valve leaflet‐inspired elastomeric scaffolds with tunable and anisotropic mechanical properties

Contact Info

Product

Resources

About