Nanofibrous poly(vinyl alcohol)/chitosan contained carbonated hydroxyapatite nanoparticles scaffold for bone tissue engineering

Januariyasa, I Komang; Ana, Ika Dewi; Yusuf, Yusril

doi:10.1016/j.msec.2019.110347

Cited by 114 publications

(84 citation statements)

References 64 publications

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“…FTIR (Thermo Nicolet iS10, Japan) was conducted to determine the functional groups of the abalone mussel shells, calcinated abalone mussel shells, synthesized HA, and HA-based HCB scaffolds. Separately, the powder and then scaffold were ground and mixed with potassium bromide (KBr) and then passed into compact tablets [ 26 ]. The FTIR instrument was operated in the range of 400–4000 cm −1 .…”

Section: Materials and Experimental Methodsmentioning

confidence: 99%

Bioceramic hydroxyapatite-based scaffold with a porous structure using honeycomb as a natural polymeric Porogen for bone tissue engineering

et al. 2021

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Background The application of bioceramic hydroxyapatite (HA) derived from materials high in calcium to tissue engineering has been of concern, namely scaffold. Scaffold pores allow for cell mobility metabolic processes, and delivery of oxygen and nutrients by blood vessel. Thus, pore architecture affects cell seeding efficiency, cell viability, migration, morphology, cell proliferation, cell differentiation, angiogenesis, mechanical strength of scaffolds, and, eventually, bone formation. Therefore, to improve the efficacy of bone regeneration, several important parameters of the pore architecture of scaffolds must be carefully controlled, including pore size, geometry, orientation, uniformity, interconnectivity, and porosity, which are interrelated and whose coordination affects the effectiveness of bone tissue engineering. The honeycomb (HCB) as natural polymeric porogen is used to pore forming agent of scaffolds. It is unique for fully interconnected and oriented pores of uniform size and high mechanical strength in the direction of the pores. The aim of this study was therefore to evaluate the effect of HCB concentration on macropore structure of the scaffolds. Methods Bioceramic hydroxyapatite (HA) was synthesized from abalone mussel shells (Halioitis asinina) using a precipitation method, and HA-based scaffolds were fabricated with honeycomb (HCB) as the porogen agent. Pore structure engineering was successfully carried out using HCB at concentrations of 10, 20, and 30 wt%. Results The Energy Dispersive X-Ray Spectroscopy (EDS) analysis revealed that the Ca/P molar ratio of HA was 1.67 (the stoichiometric ratio of HA). The Fourier Transform Infrared Spectroscopy (FTIR) spectra results for porous HA-based scaffolds and synthesized HA showed that no chemical decomposition occurred in the HA-based scaffold fabrication process. The porosity of the scaffold tended to increase when higher concentrations of HCB were added. XRD data show that the HCB was completely degraded from the scaffold material. The cell metabolic activity and morphology of the HA + HCB 30 wt% scaffold enable it to facilitate the attachment of MC3T3E1 cells on its surface. Conclusion HCB 30 wt% is the best concentration to fabricate the scaffold corresponding to the criteria for pores structure, crystallographic properties, chemical decomposition process and cell viability for bone tissue engineering.

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Section: Materials and Experimental Methodsmentioning

confidence: 99%

Bioceramic hydroxyapatite-based scaffold with a porous structure using honeycomb as a natural polymeric Porogen for bone tissue engineering

et al. 2021

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“…The most commonly used natural polymers are collagen (Tiplea et al, 2017), gelatin (Maji & Dasgupta, 2019), alginate (Ucar et al, 2019), and chitosan (Januariyasa, Ana, & Yusuf, 2020; Maji & Dasgupta, 2019; Yadav, Rhee, Park, & Hui, 2014). Synthetic polymers that are most often used in bone tissue engineering are poly(lactide‐co‐glycolide) (PLGA) (Guo, Du, Wang, Cai, & Yang, 2020), polycaprolactone (PCL) (Liu et al, 2020), poly(vinyl alcohol) (PVA) (Januariyasa et al, 2020), poly(glycolic acid) (PGA) (Takahashi, Yamaguchi, Tanimoto, Yao‐Umezawa, & Kasai, 2015), poly(lactic acid) (PLA) (Behera, Sivanjineyulu, Chang, & Chiu, 2018; Zhang et al, 2020). Chitosan (CS) is intensively investigated in the field of tissue engineering due to its unique properties, such as biodegradability, biocompatibility, adhesiveness and intrinsic antibacterial properties (Hurt, Kotha, Trivedi, & Coleman, 2015; Stevanović et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Antibacterial graphene‐based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering

Stevanović

Djošić

Janković

et al. 2020

J Biomedical Materials Res

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Electrophoretic deposition process (EPD) was successfully used for obtaining graphene (Gr)‐reinforced composite coating based on hydroxyapatite (HAP), chitosan (CS), and antibiotic gentamicin (Gent), from aqueous suspension. The deposition process was performed as a single step process at a constant voltage (5 V, deposition time 12 min) on pure titanium foils. The influence of graphene was examined through detailed physicochemical and biological characterization. Fourier transform infrared spectroscopy, field emission scanning electron microscopy, thermogravimetric analysis, X‐ray diffraction, Raman, and X‐ray photoelectron analyses confirmed the formation of composite HAP/CS/Gr and HAP/CS/Gr/Gent coatings on Ti. Obtained coatings had porous, uniform, fracture‐free surfaces, suggesting strong interfacial interaction between HAP, CS, and Gr. Large specific area of graphene enabled strong bonding with chitosan, acting as nanofiller throughout the polymer matrix. Gentamicin addition strongly improved the antibacterial activity of HAP/CS/Gr/Gent coating that was confirmed by antibacterial activity kinetics in suspension and agar diffusion testing, while results indicated more pronounced antibacterial effect against Staphylococcus aureus (bactericidal, viable cells number reduction >3 logarithmic units) compared to Escherichia coli (bacteriostatic, <3 logarithmic units). MTT assay indicated low cytotoxicity (75% cell viability) against MRC‐5 and L929 (70% cell viability) tested cell lines, indicating good biocompatibility of HAP/CS/Gr/Gent coating. Therefore, electrodeposited HAP/CS/Gr/Gent coating on Ti can be considered as a prospective material for bone tissue engineering as a hard tissue implant.

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“…CS utilization in drugs is extremely varied. However, tissue engineering (TE) and wound dressing (WD) tend to be its two primary applications [ 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 ]. It was exhibited [ 68 ] that CS might be employed to hinder fibroplasia in wound healing and to enhance cell growth and attachment.…”

Section: Chitosan (Cs)mentioning

confidence: 99%

Three-Dimensional Printing Constructs Based on the Chitosan for Tissue Regeneration: State of the Art, Developing Directions and Prospect Trends

et al. 2020

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Chitosan (CS) has gained particular attention in biomedical applications due to its biocompatibility, antibacterial feature, and biodegradability. Hence, many studies have focused on the manufacturing of CS films, scaffolds, particulate, and inks via different production methods. Nowadays, with the possibility of the precise adjustment of porosity size and shape, fiber size, suitable interconnectivity of pores, and creation of patient-specific constructs, 3D printing has overcome the limitations of many traditional manufacturing methods. Therefore, the fabrication of 3D printed CS scaffolds can lead to promising advances in tissue engineering and regenerative medicine. A review of additive manufacturing types, CS-based printed constructs, their usages as biomaterials, advantages, and drawbacks can open doors to optimize CS-based constructions for biomedical applications. The latest technological issues and upcoming capabilities of 3D printing with CS-based biopolymers for different applications are also discussed. This review article will act as a roadmap aiming to investigate chitosan as a new feedstock concerning various 3D printing approaches which may be employed in biomedical fields. In fact, the combination of 3D printing and CS-based biopolymers is extremely appealing particularly with regard to certain clinical purposes. Complications of 3D printing coupled with the challenges associated with materials should be recognized to help make this method feasible for wider clinical requirements. This strategy is currently gaining substantial attention in terms of several industrial biomedical products. In this review, the key 3D printing approaches along with revealing historical background are initially presented, and ultimately, the applications of different 3D printing techniques for fabricating chitosan constructs will be discussed. The recognition of essential complications and technical problems related to numerous 3D printing techniques and CS-based biopolymer choices according to clinical requirements is crucial. A comprehensive investigation will be required to encounter those challenges and to completely understand the possibilities of 3D printing in the foreseeable future.

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Nanofibrous poly(vinyl alcohol)/chitosan contained carbonated hydroxyapatite nanoparticles scaffold for bone tissue engineering

Cited by 114 publications

References 64 publications

Bioceramic hydroxyapatite-based scaffold with a porous structure using honeycomb as a natural polymeric Porogen for bone tissue engineering

Bioceramic hydroxyapatite-based scaffold with a porous structure using honeycomb as a natural polymeric Porogen for bone tissue engineering

Antibacterial graphene‐based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering

Three-Dimensional Printing Constructs Based on the Chitosan for Tissue Regeneration: State of the Art, Developing Directions and Prospect Trends

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