Nanomaterial‐Based Label‐Free Aptasensors

Kerman, Kağan; Tamiya, Eiichi

doi:10.1002/9780470380772.ch7

Cited by 4 publications

(4 citation statements)

References 108 publications

(104 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Signals from electrochemical oxidation of guanine bases in DNA appeared to be the most useful for practical identification of DNA and its surface states, since the guanine base has the least positive potential of all DNA bases [63][64][65]. Variation of the guanine oxidation signal associated with the DNA aptamers before and after protein ligand binding was used for analysis of IgE [66]. On carbon electrodes the aptamer for IgE gave a pronounced DPV signal of guanine oxidation at 0.8 V, which dramatically decreased upon interaction with 1-10 nM IgE.…”

Section: Electrochemical Aptamer-based Biosensors Exploiting the Inhementioning

confidence: 99%

Recent Advances in Electrochemical Aptamer-Based Sensors

Ferapontova¹,

Gothelf

2011

COC

View full text Add to dashboard Cite

Aptamers are with their selectivity and affinity for specific target ligands ideal biorecognition units for any biosensor development. The development of improved techniques for in vitro selection of aptamers with high affinities (comparable to antibodies) for target molecules has transformed aptamers from costly and restrictedly accessible species into readily synthetically available biorecognition material for almost all possible targets -from small molecules to proteins and whole cells. Unsurprisingly, the new potential of aptamers has also been applied for development of novel aptamer-based electrochemical biosensors. In this paper we overview general tendencies and recent advances in this field, exemplified by impedimetric and electrochemical conformational aptamer-based biosensors, electrochemical aptamer-based biosensors exploiting redox indicators, catalytic and quantum dot labels, and inherent redox activity of DNA, and aptamer-modified field-effect transistors.

show abstract

Section: Electrochemical Aptamer-based Biosensors Exploiting the Inhementioning

confidence: 99%

Recent Advances in Electrochemical Aptamer-Based Sensors

Ferapontova¹,

Gothelf

2011

COC

View full text Add to dashboard Cite

show abstract

“…Other examples of the use of AuNP to increase loading capacity are reported in Refs. [84][85][86][87].…”

Section: Metal Nanoparticlesmentioning

confidence: 98%

Electrochemical nanomaterial-based nucleic acid aptasensors

Palchetti

Mascini

2012

Anal Bioanal Chem

View full text Add to dashboard Cite

Recent progress in the development of electrochemical nanomaterial-aptamer-based biosensors is summarized. Aptamers are nucleic acid ligands that can be generated against amino acids, drugs, proteins, and other molecules. They are isolated from a large random library of synthetic nucleic acids by an iterative process of binding, separation, and amplification, called systematic evolution of ligands by exponential enrichment (SELEX). In this review, different methods of integrating aptamers with different nanomaterials and nanoparticles for electrochemical biosensing application are described.

show abstract

“…Detection limit of 0.5 nM was obtained. By using Au nanoparticles as the electroactive labels tagged at probing aptamer, 1 nM of thrombin has been measured on a screen-printed carbon electrode [20]. The signal was further amplified by hybridizing the aptamer with its complementary DNA, which was also labeled with gold nanoparticles [21].…”

Section: Electrochemical Aptasensors In Sandwichmentioning

confidence: 99%

A Review: Electrochemical Aptasensors with Various Detection Strategies

Cheng

et al. 2009

Electroanalysis

120

View full text Add to dashboard Cite

The combinatorial chemical synthesis offers in vitro aptamer generation with limitless aptamer selection for various analytes. Additionally, aptamer has quite many outstanding properties as the biomolecular recognition elements, thus competing with traditional affinity ligands (enzyme, antibodies, lectins etc.) in biological and medicine employments. This article reviews the advancements in electrochemical aptasensors for biomolecule monitoring, emphasizing the efficient detection strategies of sandwich structure formation using two recognition elements to capture and label target respectively, EIS measuring the electronic transfer resistance change, the aptamer conformation change triggering the electrochemical signal on/off and target molecules displacing electroactive markers.

show abstract

Nanomaterial‐Based Label‐Free Aptasensors

Cited by 4 publications

References 108 publications

Recent Advances in Electrochemical Aptamer-Based Sensors

Recent Advances in Electrochemical Aptamer-Based Sensors

Electrochemical nanomaterial-based nucleic acid aptasensors

A Review: Electrochemical Aptasensors with Various Detection Strategies

Contact Info

Product

Resources

About