Nanometer-thin TiO2 enhances skeletal muscle cell phenotype and behavior

Abstract

Background:The independent role of the surface chemistry of titanium in determining its biological properties is yet to be determined. Although titanium implants are often in contact with muscle tissue, the interaction of muscle cells with titanium is largely unknown. This study tested the hypotheses that the surface chemistry of clinically established microroughened titanium surfaces could be controllably varied by coating with a minimally thin layer of TiO 2 (ideally pico-to-nanometer in thickness) without altering the existing topographical and roughness features, and that the change in superficial chemistry of titanium is effective in improving the biological properties of titanium. Methods and results: Acid-etched microroughened titanium surfaces were coated with TiO 2 using slow-rate sputter deposition of molten TiO 2 nanoparticles. A TiO 2 coating of 300 pm to 6.3 nm increased the surface oxygen on the titanium substrates in a controllable manner, but did not alter the existing microscale architecture and roughness of the substrates. Cells derived from rat skeletal muscles showed increased attachment, spread, adhesion strength, proliferation, gene expression, and collagen production at the initial and early stage of culture on 6.3 nm thick TiO 2 -coated microroughened titanium surfaces compared with uncoated titanium surfaces. Conclusion: Using an exemplary slow-rate sputter deposition technique of molten TiO 2 nanoparticles, this study demonstrated that titanium substrates, even with microscale roughness, can be sufficiently chemically modified to enhance their biological properties without altering the existing microscale morphology. The controllable and exclusive chemical modification technique presented in this study may open a new avenue for surface modifications of titaniumbased biomaterials for better cell and tissue affinity and reaction.

Improving cytocompatibility of Co28Cr6Mo by TiO 2 coating: gene expression study in human endothelial cells

“…Sol-gel coating of Ti1.5Al25V effectively shielded fibroblasts from toxic vanadium and significantly enhanced cell viability [44]. TiO 2 coating of acid-etched titanium surfaces via slow-rate sputter deposition of molten TiO 2 nanoparticles improved attachment, proliferation and extracellular matrix deposition by osteoblast and muscle cells [45,46]. In in vivo experiments, direct attachment of soft tissues after subcutaneous implantation in rats was improved by sol-gel-derived nanoporous TiO 2 coating [47].…”

Nano-Engineered Biomaterials for Tissue Regeneration: What Has Been Achieved So Far?

“…Structural integrity was stable over 4 weeks and the AuNPs did not show signs of toxicity. In regard to coating, metal NPs are capable of promoting cellular interactions and maintaining cell presence at the scaffold surface, which enhances their functional ability (Ishizaki et al, 2011).…”