Nanoparticle‐stabilized microbubbles for multimodal imaging and drug delivery

Mørch, Ýrr; Hansen, Rune; Berg, Sigrid; Åslund, Andreas; Glomm, Wilhelm R.; Eggen, Siv; Schmid, Ruth; Johnsen, Heidi; Kubowicz, Stephan; Snipstad, Sofie; Sulheim, Einar; Hak, Sjoerd; Singh, Gurvinder; McDonagh, Birgitte Hjelmeland; Blom, Hans; Davies, Catharina de Lange; Stenstad, Per

doi:10.1002/cmmi.1639

Cited by 60 publications

(54 citation statements)

References 40 publications

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“…PBCA-NPs were synthesized by the miniemulsion process as described previously (41) and presented in the Supporting Information. Oil-in-water NEs were prepared as described previously (42) and presented in the Supporting information.…”

Section: Synthesis Of Polymeric-and Lipid-based Npsmentioning

confidence: 99%

Labeling nanoparticles: Dye leakage and altered cellular uptake

et al. 2016

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In vitro and in vivo behavior of nanoparticles (NPs) is often studied by tracing the NPs with fluorescent dyes. This requires stable incorporation of dyes within the NPs, as dye leakage may give a wrong interpretation of NP biodistribution, cellular uptake and intracellular distribution. Furthermore, NP labeling with trace amounts of dye should not alter NP properties such as interactions with cells or tissues.To allow for versatile NP studies with a variety of fluorescence-based assays, labeling of NPs with different dyes is desirable. Hence, when new dyes are introduced, simple and fast screening methods to assess labeling stability and NP-cell interactions are needed. For this purpose we have used a previously described generic flow cytometry assay; incubation of cells with NPs at 4°C and 37°C. Cell-NP interaction is confirmed by cellular fluorescence after 37°C incubation, and NP dye retention is confirmed when no cellular fluorescence is detected at 4°C.Three different NP-platforms labeled with six different dyes were screened, and a great variability in dye retention was observed. Surprisingly, incorporation of trace amounts of certain dyes was found to reduce, or even inhibit NP uptake. This work highlights the importance of thoroughly evaluating every dye-NP combination before pursuing fluorescent NP applications.3

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Section: Synthesis Of Polymeric-and Lipid-based Npsmentioning

confidence: 99%

Labeling nanoparticles: Dye leakage and altered cellular uptake

et al. 2016

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“…1) [53,54]. Several types of nanoparticles have been used for this purpose including gold nanoparticles [55], silica nanoparticles [56], polymer nanoparticles [5], and liposomal formulations [57].…”

Section: Microbubbles and Drug Carriersmentioning

confidence: 99%

Ultrasound-guided drug delivery in cancer

2017

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Recent advancements in ultrasound and microbubble (USMB) mediated drug delivery technology has shown that this approach can improve spatially confined delivery of drugs and genes to target tissues while reducing systemic dose and toxicity. The mechanism behind enhanced delivery of therapeutics is sonoporation, the formation of openings in the vasculature, induced by ultrasound-triggered oscillations and destruction of microbubbles. In this review, progress and challenges of USMB mediated drug delivery are summarized, with special focus on cancer therapy.

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“…PEG-PDLA nanoparticles were used to overcome aqueous solubility barriers of paclitaxel under US guidance [125]. Polymer nanoparticle-stabilized MBs with embedded SPIONs provide MRI/US imaging and pulse-activated nanoparticle release [126]. Gas-generating docetaxel (DTX) and Cy5.5 dye-loaded poly(CBL-PO) nanoparticles in MBs offer fluorescence/US signal and are released at tumor sites upon ultrasound irradiation through bubble burst, with much higher contrast than clinically-used Sonovue® and Definity® and higher therapeutic effects than free DTX or without US activation [127].…”

Section: Therapymentioning

confidence: 99%

Molecular Imaging in Nanotechnology and Theranostics

et al. 2017

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The “Molecular Imaging in Nanotechnology and Theranostics” (MINT) Interest Group of the World Molecular Imaging Society (WMIS) was founded in 2015 and was officially inaugurated during the 2016 World Molecular Imaging Conference (WMIC). The MINT interest group was created in response to the exponential growth of the fields of Nanotechnology and Theranostics in recent years, and the resulting need to provide a more organized and focused forum on these topics at the WMIS and the WMIC. The overarching goal of MINT is to bring together the many scientists who work on molecular imaging approaches using nanotechnology, and those that work on theranostic agents. MINT therefore represents scientists, labs, and institutes that are very diverse in their scientific backgrounds and areas of expertise, reflecting the wide array of materials and approaches that drive these fields. In this short review, we attempt to provide a condensed overview over some of the key areas covered by MINT. Given the breadth of the fields and the given space constraints, we have limited the coverage to the realm of nano-constructs, although theranostics is certainly not limited to this domain. We will also focus only on the most recent developments of the last 3-5 years, in order to provide the reader with an intuition of what is “in the pipeline” and has potential for clinical translation in the near future.

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Nanoparticle‐stabilized microbubbles for multimodal imaging and drug delivery

Cited by 60 publications

References 40 publications

Labeling nanoparticles: Dye leakage and altered cellular uptake

Labeling nanoparticles: Dye leakage and altered cellular uptake

Ultrasound-guided drug delivery in cancer

Molecular Imaging in Nanotechnology and Theranostics

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