Nanoparticles as Pseudostationary Phase in Capillary Electrochromatography/ESI-MS

Viberg, Peter; Jörntén‐Karlsson, Magnus; Petersson, Patrik; Sartipy, Peter; Nilsson, Staffan

doi:10.1021/ac0204045

Cited by 81 publications

(81 citation statements)

References 29 publications

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“…In 2002, Viberg et al [23] showed that it was possible to couple nanoparticle PSP-based CEC to ESI-MS with maintained ionization efficiency in absence of disturbing background signals and ion source contamination [24][25][26]. The technique was named continuous full filling (CFF), to distinguish it from PF that had previously been the only general technique available for coupling of PSP-based separations with ESI-MS detection (Figure 1).…”

Section: Introductionmentioning

confidence: 99%

Continuous full filling capillary electrochromatography–electrospraying chromatographic nanoparticles

et al. 2010

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Abbreviations CFFContinuous full filling IS AbstractThe influence of instrumental parameters affecting the ionization in continuous full-filling capillary electrochromatography / electrospray ionization mass spectrometry (CFF-CEC/ESI-MS) was investigated. The investigated parameters were the background electrolyte (BGE) and sheath liquid ion strength and organic modifier content, the nebulizer gas pressure, and the concentration of nanoparticles in the BGE. It was found that the nebulizer pressure had the largest influence on the separation efficiency and apparent retention. It was shown that even the lowest pressure investigated was sufficient to guide the nanoparticle flow away from the mass spectrometer inlet. A nebulizer pressure of 5 psi was found being optimal; increasing the pressure significantly decreased the separation efficiency due to the generation of a hydrodynamic flow. Generally, the ion strength of both the BGE and the sheath liquid were found to have very moderate effects on the separation of a homologous series of dialkyl phthalates. Whereas the ionization efficiency was found being unaffected by the nanoparticles, the separation efficiency was found to increase with increasing concentrations up to 3.8 mg/mL, where after it was observed to drop. The optimized method was linear over a wide concentration range and presented limits of detection and quantification more than three-fold lower than what have previously been reported using CFF-CEC/ESI-MS.3

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Section: Introductionmentioning

confidence: 99%

Continuous full filling capillary electrochromatography–electrospraying chromatographic nanoparticles

et al. 2010

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“…7,8 NPs were also used in electrophoresis techniques, either as modifiers to the wall of capillary or microchip, [9][10][11][12] or as pseudostationary phases (PSPs) in which they were added into the running buffers. [13][14][15] NPs used as PSPs can be replaced from run to run, and the stationary carry-over effects are thus avoided, favoring sample analysis in complex matrices. 16 Since the first report by Wallingford and Ewing, 17 various NPs to date have been explored.…”

Section: Introductionmentioning

confidence: 99%

“…15 Viberg et al reported that the NPs in the buffer did not enter the mass spectrometer connected to the capillary via orthogonal electrospray interface, and the analytes were detected with high sensitivity. 13 The same research group employed NPs as PSPs in separation of neutral compounds, and they reported plate numbers as high as 700000/m. 14 Chang's group employed gold nanoparticles (GNPs) as additives in CE separation of DNA fragments.…”

Section: Introductionmentioning

confidence: 99%

Electrophoretic Separation of Proteins in Capillaries Filled with Poly(ethylene oxide)-stabilized Silver Nanoparticles

Qin

Tursen

2009

ANAL. SCI.

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Poly(ethylene oxide)-stabilized silver nanoparticles (PEO-SSNPs) were synthesized and investigated for their potential use in capillary electrophoretic separation of proteins. It was found that the PEO-SSNPs were relatively stable against changes of the buffer ionic strength, while they began to aggregate with increase of buffer pH. Adding 0.024 g/L PEOSSNPs to 30 mmol/L phosphate buffer (pH 2.66) led to obvious increase in the peak heights of albumin bovine and albumin egg. Also, the separation efficiency for all the standards enhanced due to the suppressed wall-adsorption of proteins in the presence of the nanoparticles. The above experiments suggest that the PEO-SSNPs are effective pseudostationary phase for CE separation of proteins.

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“…Gold NPs which are easily prepared were separately added to aqueous electrolytes and poly (ethylene oxide) solutions to optimize resolution for small solutes, as well as DNA and proteins [24][25][26][27][28][29][30][31]. Continuous filling and partial filling of polymeric NPs that serve as pseudostationary phases are both useful for the separations of three amines by CEC, without conducting time-consuming particle packing and using retaining frits [32].…”

Section: Introductionmentioning

confidence: 99%

Capillary electrophoretic separation of biologically active amines and acids using nanoparticle‐coated capillaries

et al. 2008

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This manuscript describes dynamic coating of capillaries with poly(L-lysine) (PLL) and silica nanoparticles (SiO 2 NPs) and use of the as-prepared capillaries for the separation of biogenic amines and acids by CE in conjunction with LIF detection. The directions of EOF are controlled by varying the outmost layer of the capillaries with PLL and SiO 2 NPs, respectively. Over the pH range 3.0-5.0, the (PLL-SiO 2 NP) n -PLL capillaries have an EOF toward the anodic end and are more suitable for the separation of acids with respect to speed, while the (PLL-SiO 2 NP) n capillaries have an EOF toward the cathodic end and are more suitable for the separation of biogenic amines regarding speed and sensitivity. The separations of standard solutions containing five amines and two acids by CE with LIF detection using (PLL-SiO 2 NP) 2 -PLL and (PLL-SiO 2 NP) 3 capillaries were accomplished within 10 and 7 min, providing plate numbers of 3.8 and 5.0610 4 plates/m for 5-hydroxytryptamine (5-HT), respectively. The LODs for 5-HT and 5-hydroxyindole-3-acetic acid (5-HIAA) are 32 and 2 nM and 0.2 and 1.5 nM when using the (PLL-SiO 2 NP) 2 -PLL and (PLL-SiO 2 NP) 3 capillaries, respectively. Identification and quantification of 5-HIAA, homovanillic acid, and DL-vanillomandelic acid in urine samples from a male before and after drinking green tea were tested to validate practicality of the present approach. The results show that the (PLLSiO 2 NP) 2 -PLL capillary provides greater resolving power, while the (PLL-SiO 2 NP) 3 capillary provides better sensitivity, higher efficiency, and longer durability for the separation of the amines and acids.

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Nanoparticles as Pseudostationary Phase in Capillary Electrochromatography/ESI-MS

Cited by 81 publications

References 29 publications

Continuous full filling capillary electrochromatography–electrospraying chromatographic nanoparticles

Continuous full filling capillary electrochromatography–electrospraying chromatographic nanoparticles

Electrophoretic Separation of Proteins in Capillaries Filled with Poly(ethylene oxide)-stabilized Silver Nanoparticles

Capillary electrophoretic separation of biologically active amines and acids using nanoparticle‐coated capillaries

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