Nanoparticles for DNA Vaccine Delivery

Abstract

Nanotechnology is the development of engineered devices or materials at the micro molecular level in the nanometer range. The properties of nanoparticles that these could be designed, manufactured and introduced into the human body, have led to its application in various fields of medicine. They are being used for construction of diagnostic devices, contrast agents, analytical tools, physical therapy, drug discovery and drug delivery vehicles. The DNA vaccines have been emerged as best remedy for problematic diseases being capable of producing humoral and cellular immune responses as well as the safest vaccines so far. There are a large number of infectious diseases against which traditional vaccines failed to respond effectively. Especially, viral diseases and cancer where DNA vaccines seem to be the better option. However, the magnitude of immune responses produced by them in primates is not sufficient to be used in human beings. There is an evidence that these immune responses can be augmented by using properly structured nano-sized particles that may avoid DNA degradation and facilitate targeted and controlled delivery to antigen presenting cells. Adsorption, formulation or encapsulation with particles has been found to stabilize DNA formulations. The use of nanoparticles for vaccine delivery is a platform technology and has been applied for delivery of a variety of existing and potential vaccines successfully.

Chelerythrine and Fe3O4 Loaded Multi-Walled Carbon Nanotubes for Targeted Cancer Therapy

“…[5][6][7] Therefore current efforts are focused on developing the strategies for the targeted drug delivery. [8][9][10] With rapid development of nanotechnology, a variety of multifunctional drug targeted delivery systems have been widely applied in biomedical fields for the treatment of cancer in recent years. [11][12][13][14][15][16][17][18][19][20][21] Properties of targeted delivery and controllable release are necessary for an ideal drug delivery system, thus the drug could delivery and release in a selective and discriminatory way.…”

Development and Validation of an Automated Liquid Handling System for Sample Preparation Based on Multichannel Air Displacement Pipetting Technology

“…[13][14][15][16][17][18][19][20][21][22][23][24][25][26] With advances in nanotechnology, [27][28][29][30][31][32][33][34][35][36][37] particularly in magnetic * Authors to whom correspondence should be addressed. nanotechnology, [38][39][40][41][42][43][44][45][46][47][48][49] high-throughput liquid handling technology can greatly increase the throughput of a laboratory and save precious time for scientists. Moreover, the automated sample preparation system has improved analytical precision and accuracy.…”

Hemoglobin-Loaded Triblock PCLA-PEG-PCLA Copolymer Vesicles: A Potential Oxygen Carrier

“…10 When compared with liposomes, the polymeric vesicles have several advantages, such as enhanced stability, lower permeability, membrane thickness, surface functionality and degradation kinetics. [11][12][13][14][15][16] Numerous block copolymers with different hydrophilic and hydrophobic block lengths have been synthesized to fabricate polymeric vesicles, such and poly(ethylene glycol)-poly(lactide) (PEG-PLA), which were used as potential oxygen nanocarriers. 17 It has also been revealed by oxygen-binding assays that the encapsulation process does not affect the binding properties of Hb, and in addition, the polymeric membrane does not increase the Hb oxidation.…”