Nanoplastics as a potential environmental health factor: effects of polystyrene nanoparticles on human intestinal epithelial Caco-2 cells

Cortés, Constanza; Domenech, Josefa; Salazar, Marcela; Pastor, Susana; Marcos, Ricard; Hernández, Alba

doi:10.1039/c9en00523d

Cited by 124 publications

(82 citation statements)

References 43 publications

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“…A few studies using TiO 2 and peptide conjugated Au NPs showed accumulation of such particles in the perinuclear region of the mouse neural stem cells and malignant epithelial cell membrane, respectively 47 , 48 . Similar observations were also reported from Caco-2 cells exposed to PMMA nanoparticles and Hela cells exposed to PS NPs 31 , 49 . After 24 h, more number of PVC and PMMA NPs were accumulated at the perinuclear region of the BHk-21 cells.…”

Section: Discussionsupporting

confidence: 89%

Understanding the interactions of poly(methyl methacrylate) and poly(vinyl chloride) nanoparticles with BHK-21 cell line

Mahadevan

Valiyaveettil

2021

Sci Rep

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Microplastic and nanoplastic particles are prevalent in the environment and are beginning to enter the living system through multiple channels. Currently, little is known about the impact of plastic nanoparticles in living organisms. In order to investigate the health impact of micro- and nanoparticles of common polymers in a systematic way, luminescent plastic nanoparticles from two common polymers, polyvinyl chloride (PVC) and poly (methyl methacrylate) (PMMA) with relatively narrow size distribution are prepared using a nanoprecipitation method. As a model system, BHK-21 cells were exposed to polymer nanoparticles to understand the mode of uptake, internalization and biochemical changes inside the cells. The cellular effects of the nanoparticles were evaluated by monitoring the changes in cell viability, cell morphology, concentrations of reactive oxygen species (ROS), adenine triphosphate (ATP) and lactate dehydrogenase at different concentrations of the nanoparticles and time of exposure. PVC and PMMA nanoparticles induced a reduction in the cell viability along with a reduction of ATP and increase of ROS concentrations in a dose- and time-dependent manner. The plastic nanoparticles are internalized into the cell via endocytosis, as confirmed by Dynasore inhibition assay and colocalization with latex beads. Our findings suggest that plastic nanoparticle internalization could perturb cellular physiology and affect cell survival under laboratory conditions.

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Section: Discussionsupporting

confidence: 89%

Understanding the interactions of poly(methyl methacrylate) and poly(vinyl chloride) nanoparticles with BHK-21 cell line

Mahadevan

Valiyaveettil

2021

Sci Rep

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“…27 Other studies using different fish cell lines showed that toxicity is highly dependent on the cell line 28,29 but no data was available on any zebrafish cell line. In a recent study by Cortés et al, 27 no toxicity was reported at concentrations of up to 200 mg l −1 PS-NPs, after 24 h of treatment. It is worth mentioning that the LD50 calculation methodology is originally designed to calculate lethal doses of drugs or chemicals.…”

Section: In Vitro Toxicity Uptake and Accumulation Of Ps-nps In Zfl mentioning

confidence: 99%

Polystyrene nanoplastics accumulate in ZFL cell lysosomes and in zebrafish larvae after acute exposure, inducing a synergistic immune response in vitro without affecting larval survival in vivo

Brandts

Garcia-Ordoñez

Tort

et al. 2020

Environ. Sci.: Nano

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“…Free radicals that have been generated along the polymer chain can then bind to atmospheric oxygen and lead to the formation of polymeric peroxy radicals, while continuing to form secondary polymeric alkyl radicals [ 3 ]. Currently, the studies on oxidative stress induced by PS-NPs have been conducted mainly on animals [ 40 , 41 , 42 , 43 ], while there have been only a few in vitro studies performed [ 44 , 45 , 46 ].…”

Section: Discussionmentioning

confidence: 99%

Oxidative Properties of Polystyrene Nanoparticles with Different Diameters in Human Peripheral Blood Mononuclear Cells (In Vitro Study)

Kik

Bukowska

Krokosz

et al. 2021

IJMS

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With the ongoing commercialization, human exposure to plastic nanoparticles will dramatically increase, and evaluation of their potential toxicity is essential. There is an ongoing discussion on the human health effects induced by plastic particles. For this reason, in our work, we assessed the effect of polystyrene nanoparticles (PS-NPs) of various diameters (29, 44 and 72 nm) on selected parameters of oxidative stress and the viability of human peripheral blood mononuclear cells (PBMCs) in the in vitro system. Cells were incubated with PS-NPs for 24 h in the concentration range of 0.001 to 100 µg/mL and then labeled: formation of reactive oxygen species (ROS) (including hydroxyl radical), protein and lipid oxidation and cell viability. We showed that PS-NPs disturbed the redox balance in PBMCs. They increased ROS levels and induced lipid and protein oxidation, and, finally, the tested nanoparticles induced a decrease in PBMCs viability. The earliest changes in the PBMCs were observed in cells incubated with the smallest PS-NPs, at a concentration of 0.01 μg/mL. A comparison of the action of the studied nanoparticles showed that PS-NPs (29 nm) exhibited a stronger oxidative potential in PBMCs. We concluded that the toxicity and oxidative properties of the PS-NPs examined depended to significant degree on their diameter.

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Nanoplastics as a potential environmental health factor: effects of polystyrene nanoparticles on human intestinal epithelial Caco-2 cells

Abstract: Our results show that both nPS are easily internalized by Caco-2 cells in a concentration-dependent manner, but no relevant toxic effects are observed under the analyzed exposure conditions.

Cited by 124 publications

References 43 publications

Understanding the interactions of poly(methyl methacrylate) and poly(vinyl chloride) nanoparticles with BHK-21 cell line

Understanding the interactions of poly(methyl methacrylate) and poly(vinyl chloride) nanoparticles with BHK-21 cell line

Polystyrene nanoplastics accumulate in ZFL cell lysosomes and in zebrafish larvae after acute exposure, inducing a synergistic immune response in vitro without affecting larval survival in vivo

Oxidative Properties of Polystyrene Nanoparticles with Different Diameters in Human Peripheral Blood Mononuclear Cells (In Vitro Study)

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