Nanopore membrane-based electrochemical immunoassay

Kim, Jinseong; González-Martín, Anuncia

doi:10.1007/s10008-009-0786-5

Cited by 9 publications

(6 citation statements)

References 14 publications

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“…59−62 The ultrathin suspended membrane structure is beneficial for optical sensing devices because there is no hindering influence of the underlying substrate or support, which is the case for most currently used devices. 63 Other applications, such as electrochemical measurements 16,64,65 or ion current devices, 66,67 would also benefit the ultrathin nanopore membranes in terms of temporal and spatial resolution. Through additional material deposition, the pore size can be further reduced, resulting in conical shaped "nanofunnels."…”

Section: ■ Conclusionmentioning

confidence: 99%

“…3 They are versatile biosensing tools at the single molecule level 4,5 that can be used for measuring DNA, 6−9 RNA, 10 proteins, 11−13 carbon nanotubes, 14 nanoparticles, 15 or antibody−antigen interactions. 16 In addition, nanopores can be used as filtering or capturing device for molecules, 17 viruses, 18−20 or bacteria. 21 When covered with a lipid bilayer membrane, transport processes through the membrane can also be investigated.…”

Section: ■ Introductionmentioning

confidence: 99%

“…To gain better insight into these physiological processes, nanometer sized holes in a solid state membrane that connect two compartments can be used to mimic these biological nanopores and to study their functions. , The advantages of these synthetic nanopores over the natural ones are their high stability, size controllability, and adjustable surface properties . They are versatile biosensing tools at the single molecule level , that can be used for measuring DNA, − RNA, proteins, − carbon nanotubes, nanoparticles, or antibody–antigen interactions . In addition, nanopores can be used as filtering or capturing device for molecules, viruses, − or bacteria .…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Ultrathin Suspended Nanopores with Surface Plasmon Resonance Fabricated by Combined Colloidal Lithography and Film Transfer

Junesch

Sannomiya

2014

ACS Appl. Mater. Interfaces

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Suspended plasmonic nanopores in ultrathin film layers were fabricated through a simple and widely applicable method combining colloidal lithography and thin film transfer, which allows mass production of short-range ordered nanopore arrays on a large scale. By this combined method, mechanically stable and flexible free-standing nanopore membranes with a thickness down to 15-30 nm were produced. The plasmon resonances of the ultrathin plasmonic nanopores fabricated in AlN/Au/AlN trilayer and single layer Au membranes were tuned to lie in the vis-NIR wavelength range by properly designing their dimensions. The optical responses to the refractive index changes were tested and applied to adlayer sensing. The trilayer nanopore membrane showed a unique property to support water only on one side of the membrane, which was confirmed by the resonance shift and comparison with numerical simulation. Pore size reduction down to 10 nm can be achieved through additional material deposition. The filtering function of such pore-size-reduced conical shaped nanofunnels has also been demonstrated. The presented nanopore fabrication method offers new platforms for ultrathin nanopore sensing or filtering devices with controlled pore-size and optical properties. The film transfer technique employed in this work would enable the transformation of any substrate-based nanostructures to free-standing membrane based devices without complicated multiple etching processes.

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Section: ■ Conclusionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Ultrathin Suspended Nanopores with Surface Plasmon Resonance Fabricated by Combined Colloidal Lithography and Film Transfer

Junesch

Sannomiya

2014

ACS Appl. Mater. Interfaces

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“…Electrochemical impedance spectroscopy was used to monitor changes in ionic conductance before and after the antigen-antibody interaction. Results showed increased impedance after the specific interaction, and a linear relationship of change in impedance versus antigen concentration was recorded [ 139 ].…”

Section: Nanomaterials and Biomarkersmentioning

confidence: 99%

Nanotechnology for Early Cancer Detection

Choi

Kwak

Park

2010

Sensors

291

165

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Vast numbers of studies and developments in the nanotechnology area have been conducted and many nanomaterials have been utilized to detect cancers at early stages. Nanomaterials have unique physical, optical and electrical properties that have proven to be very useful in sensing. Quantum dots, gold nanoparticles, magnetic nanoparticles, carbon nanotubes, gold nanowires and many other materials have been developed over the years, alongside the discovery of a wide range of biomarkers to lower the detection limit of cancer biomarkers. Proteins, antibody fragments, DNA fragments, and RNA fragments are the base of cancer biomarkers and have been used as targets in cancer detection and monitoring. It is highly anticipated that in the near future, we might be able to detect cancer at a very early stage, providing a much higher chance of treatment.

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“…The integration of biomolecules into nanopores and nanochannels has been recognized as a new approach for development of simple, inexpensive and highly sensitive biomolecule detection [ 3 – 5 ]. The sensitivity, low cost, simplicity, and easy miniaturization of electrochemical detection is particularly suited to the development of highly sensitive nanopore-based biosensors [ 6 ]. The sensitivity of these nanopore-based devices is highly influenced by molecular transport of analyte molecules to reach binding receptors inside the nanopores where the dimensions of the nanopore structures (pore diameters and lengths) are critical parameters.…”

Section: Introductionmentioning

confidence: 99%

The Influence of Nanopore Dimensions on the Electrochemical Properties of Nanopore Arrays Studied by Impedance Spectroscopy

Kant

Priest

Shapter

et al. 2014

Sensors

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The understanding of the electrochemical properties of nanopores is the key factor for better understanding their performance and applications for nanopore-based sensing devices. In this study, the influence of pore dimensions of nanoporous alumina (NPA) membranes prepared by an anodization process and their electrochemical properties as a sensing platform using impedance spectroscopy was explored. NPA with four different pore diameters (25 nm, 45 nm and 65 nm) and lengths (5 μm to 20 μm) was used and their electrochemical properties were explored using different concentration of electrolyte solution (NaCl) ranging from 1 to 100 μM. Our results show that the impedance and resistance of nanopores are influenced by the concentration and ion species of electrolytes, while the capacitance is independent of them. It was found that nanopore diameters also have a significant influence on impedance due to changes in the thickness of the double layer inside the pores.

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Nanopore membrane-based electrochemical immunoassay

Cited by 9 publications

References 14 publications

Ultrathin Suspended Nanopores with Surface Plasmon Resonance Fabricated by Combined Colloidal Lithography and Film Transfer

Ultrathin Suspended Nanopores with Surface Plasmon Resonance Fabricated by Combined Colloidal Lithography and Film Transfer

Nanotechnology for Early Cancer Detection

The Influence of Nanopore Dimensions on the Electrochemical Properties of Nanopore Arrays Studied by Impedance Spectroscopy

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