Nanoscale grid based positioning system for miniature instrumented robots

International Journal of Optomechatronics

Gaiffe

et al. 2010

Section: Pseudo-periodic Encryption Of Extended 2-d Surfaces 67mentioning

confidence: 99%

Pseudo-Periodic Encryption of Extended 2-D Surfaces for High Accurate Recovery of any Random Zone by Vision

Galeano-Zea

International Journal of Optomechatronics

Gaiffe

et al. 2010

“…In this regard, different approaches have been proposed. Most of them are alphanumeric patterns that, once embedded in Petri dishes or microscope slides, offer a visual representation of ROI positioning in microscopy [10]- [14]. Those types of patterning are nowadays commercialized because they do not require a software for ROI analysis and their simplicity.…”

Section: Introductionmentioning

confidence: 99%

Visual in-plane positioning of a Labeled target with subpixel Resolution: basics and application

Zea

Zarzycki

et al. 2017

TecnoL.

Vision is a convenient tool for position measurements. In this paper, we present several applications in which a reference pattern can be defined on the target for a priori knowledge of image features and further optimization by software. Selecting pseudoperiodic patterns leads to high resolution in absolute phase measurements. This method is adapted to position encoding of live cell culture boxes. Our goal is to capture each biological image along with its absolute highly accurate position regarding the culture box itself. Thus, it becomes straightforward to find again an already observed region of interest when a culture box is brought back to the microscope stage from the cell incubator where it was temporarily placed for cell culture. In order to evaluate the performance of this method, we tested it during a wound healing assay of human liver tumor-derived cells. In this case, the procedure enabled more accurate measurements of the wound healing rate than the usual method. It was also applied to the characterization of the in-plane vibration amplitude from a tapered probe of a shear force microscope. The amplitude was interpolated by a quartz tuning fork with an attached pseudo-periodic pattern. Nanometer vibration amplitude resolution is achieved by processing the pattern images. Such pictures were recorded by using a common 20x magnification lens.

“…Although those types of patterning are nowadays commercialized and used by many biologists, the allowed performances are coarse and constitute a major limiting factor for high-accurate analysis of cellular events. This problem has been addressed by diverse authors who proposed several approaches that, in most cases, require the patterning of small-sized features [9]- [13]. As an example, we can reference the pattern presented by Dominic et al [9].…”

Section: Introductionmentioning

confidence: 99%

Microfabrication of position reference patterns onto glass microscope slides for high-accurate analysis of dynamic cellular events

Galeano

Zarzycki

et al. 2017

TecnoL.

Glass microscopes slides are widely used as in situ base-substrates carrying diverse micro-fabricated systems or elements. For such purposes, the micro-fabrication process consists in transferring a pre-defined design onto the substrate made of a glass microscope slide. This is known as patterning, which is a technique that can also be used in transferring specific designs that allows region of interest (ROI) recovery under the microscope. In those cases, two main challenges appear: 1) Disturbances in light transmission should remain minimum to keep the high quality of observation of the object of interest under the microscope. 2) The pattern-size should then be small enough but, however, larger than the diffraction limit to be observable satisfactorily for positioning purposes. In this article, we present the procedures involved in the microfabrication of Pseudo-Periodic Patterns (PPP) encrypting the absolute position of an extended area. Those patterns are embedded in Pétri dishes in order to allow the highaccurate retrieval of absolute position and orientation. The presented microfabrication is based in a technique known as lift-off, which after parameter adjustment, allows the obtaining of PPP fulfilling the two previously mentioned requirements. The results report on PPP realized on glass microscope slides and composed by 2µm side dots made of aluminum with a thickness of 30nm.