Nanosilver on nanostructured silica: Antibacterial activity and Ag surface area

Sotiriou, Georgios A.; Teleki, Alexandra; Camenzind, Adrian; Krumeich, Frank; Meyer, Andreas; Panke, Sven; Pratsinis, Sotiris E.

doi:10.1016/j.cej.2011.01.099

Cited by 131 publications

(114 citation statements)

References 37 publications

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“…It is difficult to differentiate between antibacterial effects of ionic silver and Ag nanoparticles as all nanoparticles release a degree of silver ions [15,16]. The antimicrobial effect of silver is thought to be the combination of several mechanisms of action; for example, silver ions and small silver nanoparticles (Ag-NPs) (less than 10nm) have been shown capable of entering the bacterial cytoplasm and inhibit cell growth [17].…”

Section: Introductionmentioning

confidence: 99%

Biogenic synthesis of antimicrobial silver nanoparticles capped with l-cysteine

Perni

Hakala

Prokopovich

2014

Colloids and Surfaces A: Physicochemical and Engineering Aspect

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The number of medical applications of silver nanoparticles is constantly expanding due to their high bactericidal properties coupled with low toxicity towards mammalian cells. Because of this expanding use of silver nanoparticles, novel methods of synthesis have been developed in order to achieve nanoparticles preparation through inexpensive and environmentally friendly processes; biogenic synthesis of silver nanoparticles is an approach that meets those requirements.In this work, E. coli cultures centrigugates were used to synthesis L-cysteine capped silver nanoparticles. The ratio between AgNO 3 and L-cysteine has been proven to affect the size of the nanoparticles: higher amount of L-cysteine returns smaller nanoparticles, but does not affect the percentage of organic matter in the nanoparticles as determined through TGA.The silver nanoparticles prepared had antimicrobial activity against E. coli and S. aureus with Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) independent of the nanoparticles size; for both bacterial species the values of MIC and MBC were the same, 0.0432 mg/ml for E. coli and 0.0216 mg/ml for S. aureus.

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Section: Introductionmentioning

confidence: 99%

Biogenic synthesis of antimicrobial silver nanoparticles capped with l-cysteine

Perni

Hakala

Prokopovich

2014

Colloids and Surfaces A: Physicochemical and Engineering Aspect

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“…[5][6][7] The extremely strong broad-spectrum antimicrobial property of AgNPs is the key direction for the improvement of AgNPs-based biomedical products, including bandages, catheters, antiseptic sprayers, textiles, and food storage containers. and pyrolysis; 21 of these, chemical reduction has been the most common route to synthesize nanosilver. Three main components, namely organic and inorganic reducing agents, capping agents or stabilizers, and metal precursors or silver salts, are used in this method (Figure 2).…”

Section: Introductionmentioning

confidence: 99%

Nanosilver: new ageless and versatile biomedical therapeutic scaffold

Khan¹,

Saleh²,

Wahab³

et al. 2018

IJN

170

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Silver nanotechnology has received tremendous attention in recent years, owing to its wide range of applications in various fields and its intrinsic therapeutic properties. In this review, an attempt is made to critically evaluate the chemical, physical, and biological synthesis of silver nanoparticles (AgNPs) as well as their efficacy in the field of theranostics including microbiology and parasitology. Moreover, an outlook is also provided regarding the performance of AgNPs against different biological systems such as bacteria, fungi, viruses, and parasites (leishmanial and malarial parasites) in curing certain fatal human diseases, with a special focus on cancer. The mechanism of action of AgNPs in different biological systems still remains enigmatic. Here, due to limited available literature, we only focused on AgNPs mechanism in biological systems including human (wound healing and apoptosis), bacteria, and viruses which may open new windows for future research to ensure the versatile application of AgNPs in cosmetics, electronics, and medical fields.

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“…7 Several groups have addressed the technological problem of embedding silver nanoparticles into matrices. 8,9 The goal of this approach is to control the release of silver ions when the matrices are placed in contact with biological fluids. In particular, plasmapolymerized hexamethyldisiloxane (PP-HMDSO or, briefly, PP) was investigated as a coating material deposited on titanium dental implants.…”

Section: Introductionmentioning

confidence: 99%

Dental implants coated with a durable and antibacterial film

Vogel

Westphal

Salz

et al. 2015

Surface Innovations

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Dental implants coated with a durable and antibacterial film Vogel et al. embedded between two layers of PP by means of subsequent plasma polymerization and metal evaporation steps. The double PP/Ag/PP layer can be directly deposited on titanium implants, whose in vivo biocompatibility within soft tissues has been investigated in Ref. 13 .Building up on these previous results, in this article, we investigate the performances of a similar PP/Ag/PP-layered coating material (briefly, PP+Ag) toward the adhesion of bacteria and eukaryotic cells in vitro. In particular, besides HeLa cellular models, the adhesion behavior of fibroblasts and osteoblasts will be addressed. With respect to the original coating procedure, 12,13 physical vapor deposition is used instead of evaporation to deposit the silver nanoparticle layer. The advantage of the physical vapor deposition is a better control and reproducibility of the nanoparticle distribution and a lesser degree of nanoparticle agglomeration. We test the coating layer resistance to sterilization and implantation into bone tissue (Section 3.2), optimize the plasma deposition parameters to guarantee eukaryotic adhesion (Section 3.3) and check the successful bacterial deterrence of the coating in fluorescence microscopy images (Section 3.4). Materials and methods Plasma polymerization and layer formationThe plasma polymerization and deposition of silver nanoparticles are performed in the same plasma reactor chamber. A sample holder with rotating platforms is mounted inside a 125-liter vacuum chamber. The chamber comprises an electrode of rectangular shape and a silver target situated behind a shutter system. Gas inlets for the HMDSO precursor (Wacker, Munich, Germany), argon, oxygen and hydrogen (Linde AG, Pullach, Germany) and a pumping system are connected to the chamber walls. In this study, three different coating layers are investigated: (a) A 2000-nm-thick polymer coating without silver particles, which possesses cell-repelling properties (PP2000); (b) a 100-nm-thick coating without silver particles, which promotes eukaryotic adhesion (PP100) and (c) the same coating including silver nanoparticles to inhibit bacterial growth but not eukaryotic adhesion (PP100+Ag). The coating layers are produced as follows: After decreasing the chamber pressure below 10 −4 mbar, HMDSO and oxygen are injected to a working pressure of 10 −2 to 10 −3 mbar. Applying a power of 160 W for 120 s, the plasma is ignited, and a 50-nm-thick polymer preconditioning layer is formed. The 2000-nm-thick coating is prepared at 120 W and oxygen to HMDSO gas flow ratio of 1:2. On top of the preconditioning layer, either the plasma polymerization is carried on until the desired thickness is reached (e.g. 100 nm for PP100), or an intermediate layer of silver nanoparticles is deposited by applying a power of 200 W for 60 s between the silver target and the chamber walls.With this procedure, we coat sand-blasted and etched titanium dental implant screws (11·5-mm length, 5·5-mm diameter ) as well as titaniu...

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Nanosilver on nanostructured silica: Antibacterial activity and Ag surface area

Cited by 131 publications

References 37 publications

Biogenic synthesis of antimicrobial silver nanoparticles capped with l-cysteine

Biogenic synthesis of antimicrobial silver nanoparticles capped with l-cysteine

Nanosilver: new ageless and versatile biomedical therapeutic scaffold

Dental implants coated with a durable and antibacterial film

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