Nanosphere and microsphere uptake via Peyer's patches: observation of the rate of uptake in the rat after a single oral dose

Jani, Praful U.; McCarthy, D.E.; Florence, Alexander T.

doi:10.1016/0378-5173(92)90202-d

Cited by 153 publications

(63 citation statements)

References 16 publications

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“…The mechanisms of oral uptake include diffusion of particles through mucus and accessibility to enterocyte surface, epithelial interaction, cellular trafficking, exocytosis and systemic dissemination (Hou et al, 2009). Nanoparticles are preferentially absorbed via "M-cells" in the Peyer''s patches by the process of endocytosis (lymphoid uptake), thereby delivering the drug loaded particles directly into systemic circulation through the lymphatics and circumventing the firstpass metabolism (Jani et al, 1992;Desai et al, 1996). Lymphatic uptake is influenced by lipid nature, chain length, and hydrophobicity of nanoparticles (Hussain et al, 2001;Nordskog et al, 2001).…”

Section: Pharmacokineticsmentioning

confidence: 99%

Preparation andin vitro,in vivoevaluations of norfloxacin-loaded solid lipid nanopartices for oral delivery

et al. 2011

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Section: Pharmacokineticsmentioning

confidence: 99%

Preparation andin vitro,in vivoevaluations of norfloxacin-loaded solid lipid nanopartices for oral delivery

et al. 2011

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“…It is generally known that nanoparticles are better absorbed than microparticles. 55 For instance, according to Desai et al, 56 100 nm nanoparticles in rats were 15-to 250-fold better absorbed than 500 nm and 10 µm particles, respectively. Nevertheless, many reports describe that both APCs and PP M-cells are able to internalize particles up to 10 µm through phagocytosis or macropinocytosis.…”

Section: Vaccine Designmentioning

confidence: 99%

Recent advances in oral vaccine development

Smet

Allais

Cuvelier

2014

Human Vaccines & Immunotherapeutics

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“…Among the several physical properties of nanoparticles for their influence on absorption and distribution, particle size would be one of the most important factors in determining the kinetics and distribution upon administration into the body. [19][20][21][22][23][24][25] For example, Florence et al reported that polystyrene particles with a smaller diameter of 50 and 100 nm could be easily absorbed through Peyer's patches and villi of the GI tract in comparison with larger particles with diameters of 300 and 3,000 nm. 26,27 The distribution of nanoparticles in the liver may be due to the fact that the liver possesses many sinusoidal endothelial cells and Kupffer cells, and it is the major organ for removing circulating foreign materials.…”

mentioning

confidence: 99%

Optical imaging of absorption and distribution of RITC-SiO2 nanoparticles after oral administration

Lee

Lee²,

Kim³

et al. 2014

IJN

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Purpose In this study, we investigated the absorption and distribution of rhodamine B isothiocyanate (RITC)-incorporated silica oxide nanoparticles(SiNPs) (RITC-SiNPs) after oral exposure, by conducting optical imaging, with a focus on tracking the movement of RITC-SiNPs of different particle size and surface charge. Methods RITC-SiNPs (20 or 100 nm; positively or negatively charged) were used to avoid the dissociation of a fluorescent dye from nanoparticles via spontaneous or enzyme-catalyzed reactions in vivo. The changes in the nanoparticle sizes and shapes were investigated in an HCl solution for 6 hours. RITC-SiNPs were orally administered to healthy nude mice at a dose of 100 mg/kg. Optical imaging studies were performed at 2, 4, and 6 hours after oral administration. The mice were sacrificed at 2, 4, 6, and 10 hours post-administration, and ex vivo imaging studies were performed. Results The RITC-SiNPs were stable in the HCl solution for 6 hours, without dissociation of RITC from the nanoparticles and without changes in size and shape. RITC-SiNPs flowed into the small intestine from the stomach and gradually moved along the gut during the experiment. In the ex vivo imaging studies, optical signals were observed mostly in the lungs, liver, pancreas, and kidneys. The orally administered RITC-SiNPs, which were absorbed in the systemic circulation, were eliminated from the body into the urine. The 20 nm RITC-SiNPs showed higher uptake in the lungs than the 100 nm RITC-SiNPs. The distribution of the 100 nm RITC-SiNPs in the liver was higher than that of the 20 nm RITC-SiNPs, but the differences in the surface charge behavior were imperceptible. Conclusion We demonstrated that the movement of RITC-SiNPs after oral exposure could be traced by optical imaging. Optical imaging has the potential to provide valuable information that will help in understanding the behavior of SiNPs in the body following exposure.

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Nanosphere and microsphere uptake via Peyer's patches: observation of the rate of uptake in the rat after a single oral dose

Cited by 153 publications

References 16 publications

Preparation andin vitro,in vivoevaluations of norfloxacin-loaded solid lipid nanopartices for oral delivery

Preparation andin vitro,in vivoevaluations of norfloxacin-loaded solid lipid nanopartices for oral delivery

Recent advances in oral vaccine development

Optical imaging of absorption and distribution of RITC-SiO2 nanoparticles after oral administration

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