Nanotube Molecular Transporters:  Internalization of Carbon Nanotube−Protein Conjugates into Mammalian Cells

Kam, Nadine Wong Shi; Jessop, Theodore C.; Wender, Paul A.; Dai, Hongjie

doi:10.1021/ja0486059

Cited by 1,238 publications

(387 citation statements)

References 21 publications

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“…26,32 Some experimental observations had shown that very small nanoparticles and nanotubes, with size below the supposed lower critical radius, were found inside cells. [58][59][60] This observation seemed to contrast with both other experimental observations, as well as with predictions from theory and simulations.…”

Section: Predicting and Controlling Nanoparticles Endocytosiscontrasting

confidence: 74%

Theory, simulations and the design of functionalized nanoparticles for biomedical applications: A Soft Matter Perspective

Angioletti‐Uberti

2017

npj Comput Mater

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Functionalised nanoparticles for biomedical applications represents an incredibly exciting and rapidly growing field of research. Considering the complexity of the nano-bio interface, an important question is to what extent can theory and simulations be used to study these systems in a realistic, meaningful way. In this review, we will argue for a positive answer to this question. Approaching the issue from a "Soft Matter" perspective, we will consider those properties of functionalised nanoparticles that can be captured within a classical description. We will thus not concentrate on optical and electronic properties, but rather on the way nanoparticles' interactions with the biological environment can be tuned by functionalising their surface and exploited in different contexts relevant to applications. In particular, we wish to provide a critical overview of theoretical and computational coarsegrained models, developed to describe these interactions and present to the readers some of the latest results in this fascinating area of research.

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Section: Predicting and Controlling Nanoparticles Endocytosiscontrasting

confidence: 74%

Theory, simulations and the design of functionalized nanoparticles for biomedical applications: A Soft Matter Perspective

Angioletti‐Uberti

2017

npj Comput Mater

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“…Carbon nanotubes have greatly advanced these fields. Similarly as other nanoparticles, carbon nanotubes can be readily taken up by cells and are noncytotoxic within a certain concentration limit if suitably functionalized [25] [26]. The main finding of this study was that HTT2800 has a significant effect on intracellular lipid accumulation in C2C12 cells.…”

Section: Discussionmentioning

confidence: 69%

Nanoparticle-mediated intracellular lipid accumulation during C2C12 cell differentiation

Tsukahara¹,

Haniu²

2011

Biochemical and Biophysical Research Communications

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In this report, we sought to elucidate whether multiwall carbon nanotubes are involved in the modulation of the proliferation and differentiation of the skeletal muscle cell line C2C12. Skeletal muscle is a major mass peripheral tissue that accounts for 40% of total body weight and 50% of energy consumption. We focused on the differentiation pathway of myoblasts after exposure to a vapor-grown carbon fiber, HTT2800, which is one of the most highly purified carbon nanotubes.This treatment leads in parallel to the expression of a typical adipose differentiation program. We found that HTT2800 stimulated intracellular lipid accumulation in C2C12 cells. We have also shown by quantified PCR analysis that the expression of adipose-related genes was markedly upregulated during HTT2800 exposure. Taken together, these results suggest that HTT2800 specifically converts the differentiation pathway of C2C12 myoblasts to that of adipoblast-like cells.

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“…suggested that an energy-dependent endocytosis mechanism is responsible for the uptake of carbon nanotubes [17]. Endocytosis is the process by which cells absorb molecules or substances from outside the cell by engulfing them with the cell membrane.…”

Section: Discussionmentioning

confidence: 99%

Cellular cytotoxic response induced by highly purified multi-wall carbon nanotube in human lung cells

Tsukahara

Haniu

2011

Mol Cell Biochem

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Carbon nanotubes, a promising nanomaterial with unique characteristics, have applications in a variety of fields. The cytotoxic effects of carbon nanotubes are partially due to the induction of oxidative stress; however, the detailed mechanisms of nanotube cytotoxicity and their interaction with cells remain unclear. In the present study, we focus on the acute toxicity of vapor-grown carbon fiber, HTT2800, which is one of the most highly purified multi-wall carbon nanotube (MWCNT) by high-temperature thermal treatment. We exposed human bronchial epithelial cells (BEAS-2B) to HTT2800 and measured the cellular uptake, mitochondrial function, cellular LDH release, apoptotic signaling, reactive oxygen species (ROS) generation and pro-inflammatory cytokine release. The HTT2800-exposed cells showed cellular uptake of the carbon nanotube, increased cell death, enhanced DNA damage and induced cytokine release. However, the exposed cells showed no obvious intracellular ROS generation. These cellular and molecular findings suggest that HTT2800 could cause a potentially adverse inflammatory response in BEAS-2B cells.

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Nanotube Molecular Transporters: Internalization of Carbon Nanotube−Protein Conjugates into Mammalian Cells

Cited by 1,238 publications

References 21 publications

Theory, simulations and the design of functionalized nanoparticles for biomedical applications: A Soft Matter Perspective

Theory, simulations and the design of functionalized nanoparticles for biomedical applications: A Soft Matter Perspective

Nanoparticle-mediated intracellular lipid accumulation during C2C12 cell differentiation

Cellular cytotoxic response induced by highly purified multi-wall carbon nanotube in human lung cells

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