Native Microbial Inoculants for the Management of Meloidogyne incognita in Withania somnifera cv. Poshita

Isolation and characterization of endophytic fungi having plant growth promotion traits that biosynthesizes bacosides and withanolides under in vitro conditions

“…The amplified PCR products were purified using PCR Cleanup Kit (Mol Bio, Himedia) by following the instructions mentioned by manufacturers. The PCR product obtained was sequenced by 3130 xl Genetic Analyzer (Applied Biosystems) using sequencing kit (Applied Biosystems, USA) and primer [ 11 , 16 ]. The resultant sequence thus obtained was analyzed by nucleotide BLAST ( https://blast.ncbi.nlm.nih.gov/ ).…”

“…Nearly 25 ng of genomic DNA and 5 pmol of aforementioned primers were used for amplification purpose. The amplifications of ribosomal gene sequence were performed using Mastercyler gradient (Eppendorf) programmed as 95 °C for 5 min; 32 cycle at 95 °C for 30 s, 55 °C for 30 s, 72 °C for 1 min, and 72 °C for 10 min; and 4 °C for [11,16]. The resultant sequence thus obtained was analyzed by nucleotide BLAST (https:// blast.…”

“…Furthermore, the microbes with PGP potentials either endophytic or rhizospheric, supports plant growth and development with secretion of plant growth promoting enzymes [52]. They (microbes) also contribute in enhancement [1,11,52] and modulation of secondary metabolites in planta [12]. Therefore, microbes with such potentials will be beneficial for targeted enhanced metabolite productions.…”

“…Similarly, Withania somnifera (Ashwagandha) is regarded as Indian ginseng with potential therapeutic values [ 10 ] for improving body strength and immune systems, anti-aging, hepatic and cardiac cells protection, control cholesterol level, antipyretic, antiulcer, hemopoietic, etc. [ 11 , 12 ]. The therapeutic potentials of W. somnifera are due to the presence of terpenoids saponins collectively known as withanolides which include Withanolide A, Withanolide B, and Withaferrin A.…”

Diseases of Medicinal and Aromatic Plants: Insights in Nematode Biomanagement

“…Use of different bio-organics viz. farm yard manures (Fym), cow urine, neem cake, vermicompost, T. harzianum, Paecilomyces lilacinus separately and in dual combinations against root-knot disease of W. somnifera and successful management of root-knot nematode on this crop were achieved (Saikia et al, 2013, Singh et al, 2016aTiwari et al, 2016;Gupta et al, 2016). Similarly large number of medicinal and aromatic plants were also affected by variety of plant parasitic nematode and have severe impact on production of plant secondary metabolites (Gupta and Pandey, 2015;Singh et al, 2016b;Gupta et al, 2017a, b;Tiwari et al, 2017).…”