Natronorubrum halophilum sp. nov. isolated from two inland salt lakes

Tao, Cong-Qi; Ding, Yuping; Zhao, Yang-Jie; Cui, Heng‐Lin

doi:10.1007/s12275-020-9514-8

Cited by 23 publications

(8 citation statements)

References 38 publications

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“…Comparative property of Salinadaptatus with the related genera from the family Natrialbaceae. The comparative data were taken from[29][30][31]. Number of species are showed in parenthesis…”

mentioning

confidence: 99%

Salinadaptatus halalkaliphilus gen. nov., sp. nov., a haloalkaliphilic archaeon isolated from salt pond in Inner Mongolia Autonomous Region, China

Xue

Zuo

Zhou

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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A haloalkaliphilic strain XQ-INN 246T was isolated from the sediment of a salt pond in Inner Mongolia Autonomous Region, China. Cells of the strain were rods, motile and strictly aerobic. The strain was able to grow in the presence of 2.6–5.3 M NaCl (optimum concentration is 4.4 M) at 30–50 °C (optimum temperature is 42 °C) and pH 7.0–10.0 (optimum pH is 8.0–8.5). The whole genome sequencing of strain XQ-INN 246T revealed a genome size of 4.52 Mbp and a DNA G+C content of 62.06 mol%. Phylogenetic tree based on 16S rRNA gene sequences and concatenated amino acid sequences of 122 single-copy conserved proteins revealed a robust lineage of the strain XQ-INN 246T with members of related genera of the family Natrialbaceae . The strain possessed the polar lipids of phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester. No glycolipids were detected. Based on phylogenetic analysis, phenotypic characteristics, chemotaxonomic properties and genome relatedness, the isolate was proposed as the type strain of a novel species of a new genus within the family Natrialbaceae, for which the name Salinadaptatus halalkaliphilus gen. nov., sp. nov. is proposed. The type strain is XQ-INN 246T (=CGMCC 1.16692T=JCM 33751T).

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“…Comparative property of Salinadaptatus with the related genera from the family Natrialbaceae. The comparative data were taken from[29][30][31]. Number of species are showed in parenthesis…”

mentioning

confidence: 99%

Salinadaptatus halalkaliphilus gen. nov., sp. nov., a haloalkaliphilic archaeon isolated from salt pond in Inner Mongolia Autonomous Region, China

Xue

Zuo

Zhou

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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“…The pH and salinity of the brine sample were 7.5 and 12 %, respectively. Two media, neutral haloarchaeal medium (NHM) [7] and haloarchaeal complex medium (HCM7) [8], were used for the isolation and cultivation of halophilic archaea strains, respectively. These media were solidified with 1.5 % purified agar.…”

Section: Isolation and Cultivation Of Halophilic Archaeamentioning

confidence: 99%

Halocatena salina sp. nov., a filamentous halophilic archaeon isolated from Aiding Salt Lake

Zheng

Sun

et al. 2022

International Journal of Systematic and Evolutionary Microbiology

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A filamentous cell-shaped halophilic archaeon (strain AD-1T) was isolated from Aiding Salt Lake, PR China. Its colonies on HCM7 agar plates were pinkish white, 1–4 mm (diameter), elevated and round. The optimum conditions for growth were observed at 42 °C, 4.3 M NaCl, 0.01 M MgCl2 and pH 7. Strain AD-1T could hydrolyse Tween 60, Tween 80, starch and gelatin. Phylogenetic analysis based on 16S rRNA gene, rpoB′ and the concatenated 484 single-copy orthologous proteins revealed that strain AD-1T formed a clade with Halocatena pleomorpha SPP-AMP-1T. The average nucleotide identity and in silico DNA–DNA hybridization values between strain AD-1T and Halocatena pleomorpha SPP-AMP-1T were both below the species delineation thresholds (95~96 and 70 %, respectively). The major phospholipids of strain AD-1T were phosphatidic acid, phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester, while the major glycolipids were sulphated galactosyl mannosyl glucosyl diether, galactosyl mannosyl glucosyl diether and glucosyl mannosyl glucosyl diether. The phenotypic, phylogenetic and genome-based analyses suggested that strain AD-1T (=CGMCC 1.13724T=JCM 32960T) represents a novel species, for which the name Halocatena salina sp. nov. is proposed.

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“…Neutral-halophilic (pH7) and alkali-halophilic (pH9) media can be simultaneously prepared to cultivate neutral and alkaliphilic halophilic archaea that may exist in the same environmental sample (Tao et al 2020). After one month or even half a year of long-term cultivation, the groups that prefer nutrient-rich and those like low-concentration or simple nutrition can have the opportunity to grow and form colonies.…”

Section: Media Problems and Solutions Regarding Cultivation Of Halopmentioning

confidence: 99%

Cultivation of halophilic archaea (class Halobacteria) from thalassohaline and athalassohaline environments

Cui

Dyall‐Smith

2021

Mar Life Sci Technol

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As a group, the halophilic archaea (class Halobacteria) are the most salt-requiring and salt-resistant microorganisms within the domain Archaea. Halophilic archaea flourish in thalassohaline and athalassohaline environments and require over 100-150 g/L NaCl for growth and structural stability. Natural hypersaline environments vary in salt concentration, chemical composition and pH, and occur in climates ranging from tropical to polar and even under-sea. Accordingly, their resident haloarchaeal species vary enormously, as do their individual population compositions and community structures. These diverse halophilic archaeal strains are precious resources for theoretical and applied research but assessing their taxonomic and metabolic novelty and diversity in natural environments has been technically difficult up until recently. Environmental DNA-based high-throughput sequencing technology has now matured sufficiently to allow inexpensive recovery of massive amounts of sequence data, revealing the distribution and community composition of halophilic archaea in different hypersaline environments. While cultivation of haloarchaea is slow and tedious, and only recovers a fraction of the natural diversity, it is the conventional means of describing new species, and provides strains for detailed study. As of the end of May 2020, the class Halobacteria contains 71 genera and 275 species, 49.8% of which were first isolated from the marine salt environment and 50.2% from the inland salt environment, indicating that both thalassohaline and athalassohaline environments contain diverse halophilic archaea. However, there remain taxa that have not yet been isolated in pure culture, such as the nanohaloarchaea, which are widespread in the salt environment and may be one of the hot spots in the field of halophilic archaea research in the future. In this review, we focus on the cultivation strategies that have been used to isolate extremely halophilic archaea and point out some of the pitfalls and challenges.

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Natronorubrum halophilum sp. nov. isolated from two inland salt lakes

Cited by 23 publications

References 38 publications

Salinadaptatus halalkaliphilus gen. nov., sp. nov., a haloalkaliphilic archaeon isolated from salt pond in Inner Mongolia Autonomous Region, China

Salinadaptatus halalkaliphilus gen. nov., sp. nov., a haloalkaliphilic archaeon isolated from salt pond in Inner Mongolia Autonomous Region, China

Halocatena salina sp. nov., a filamentous halophilic archaeon isolated from Aiding Salt Lake

Cultivation of halophilic archaea (class Halobacteria) from thalassohaline and athalassohaline environments

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