Nattokinase Crude Extract Enhances Cutaneous Wound Healing

Zhang, Bin; Liu, Yuwan; Jiang, Qian; Zhao, Ming; Zeng, Jing; Liu, Lihua; Xu, Xiao Dong; Yan, Yongmin; Gong, Aihua

doi:10.1166/jbt.2017.1697

Cited by 3 publications

(2 citation statements)

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“…natto) ( Sumi et al, 1987 ; Ueda, 1989 ; Fujita et al, 1993 ; Osawa and Matsumoto, 1997 ; Fujita et al, 2022 ). B. subtilis natto produces several molecules associated with human health ( Su et al, 2020 ), including large amounts of vitamin K2 (menaquinone-7), important for bone robustness ( Ikeda and Doi, 1990 ; Tsukamoto et al, 2000 ; Hu et al, 2020 ; Wu et al, 2021 ); pyrroloquinoline quinone (PQQ), a natural antioxidant with energizing and anti-fatigue effects ( Nakano et al, 2012 , 2015 ); nattokinase, a protease conferring protection against high blood pressure ( Sumi et al, 1987 ; Fujita et al, 1993 ; Kim et al, 2008 ), ischemic stroke ( Urano et al, 2001 ; Hsu et al, 2009 ; Murakami et al, 2012 ; Ji et al, 2014 ; Pham et al, 2020 ; Suzuki et al, 2023 ), cardiovascular disease ( Urano et al, 2001 ; Hsu et al, 2009 ; Murakami et al, 2012 ; Weng et al, 2017 ; Suzuki et al, 2023 ), with anti-neurodegenerative ( Hsu et al, 2009 ; Kumar et al, 2017 ) and anti-celiac effects ( Wei et al, 2016 ); and cutaneous wound healing properties ( Zhang et al, 2019 ). In addition, B. subtilis produces natural antimicrobials (lipopeptide antibiotics) that demonstrated activity against pathogenic bacteria ( Ongena et al, 2005 ; Cao et al, 2009 ; Piewngam et al, 2018 ; Piewngam and Otto, 2020 ), fungi ( Yu et al, 2002 ; Cawoy et al, 2015 ; Bartolini et al, 2019 ), viruses ( Itokawa et al, 1994 ; Vollenbroich et al, 1997 ; Jung et al, 2000 ), and cancer cells ( Nagata et al, 2002 ; Wang et al, 2007 ; Cao et al, 2009 ; Myung et al, 2009 ; Zhang et al, 2015 ; Lu et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Probiotic properties of Bacillus subtilis DG101 isolated from the traditional Japanese fermented food nattō

Leñini,

Rodriguez Ayala,

Goñi

et al. 2023

Front. Microbiol.

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Spore-forming probiotic bacteria offer interesting properties as they have an intrinsic high stability, and when consumed, they are able to survive the adverse conditions encountered during the transit thorough the host gastrointestinal (GI) tract. A traditional healthy food, nattō, exists in Japan consisting of soy fermented by the spore-forming bacterium Bacillus subtilis natto. The consumption of nattō is linked to many beneficial health effects, including the prevention of high blood pressure, osteoporosis, and cardiovascular-associated disease. We hypothesize that the bacterium B. subtilis natto plays a key role in the beneficial effects of nattō for humans. Here, we present the isolation of B. subtilis DG101 from nattō and its characterization as a novel spore-forming probiotic strain for human consumption. B. subtilis DG101 was non-hemolytic and showed high tolerance to lysozyme, low pH, bile salts, and a strong adherence ability to extracellular matrix proteins (i.e., fibronectin and collagen), demonstrating its potential application for competitive exclusion of pathogens. B. subtilis DG101 forms robust liquid and solid biofilms and expresses several extracellular enzymes with activity against food diet-associated macromolecules (i.e., proteins, lipids, and polysaccharides) that would be important to improve food diet digestion by the host. B. subtilis DG101 was able to grow in the presence of toxic metals (i.e., chromium, cadmium, and arsenic) and decreased their bioavailability, a feature that points to this probiotic as an interesting agent for bioremediation in cases of food and water poisoning with metals. In addition, B. subtilis DG101 was sensitive to antibiotics commonly used to treat infections in medical settings, and at the same time, it showed a potent antimicrobial effect against pathogenic bacteria and fungi. In mammalians (i.e., rats), B. subtilis DG101 colonized the GI tract, and improved the lipid and protein serum homeostasis of animals fed on the base of a normal- or a deficient-diet regime (dietary restriction). In the animal model for longevity studies, Caenorhabditis elegans, B. subtilis DG101 significantly increased the animal lifespan and prevented its age-related behavioral decay. Overall, these results demonstrate that B. subtilis DG101 is the key component of nattō with interesting probiotic properties to improve and protect human health.

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Section: Introductionmentioning

confidence: 99%

Probiotic properties of Bacillus subtilis DG101 isolated from the traditional Japanese fermented food nattō

Leñini,

Rodriguez Ayala,

Goñi

et al. 2023

Front. Microbiol.

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“…Recent study revealed that NK exerts its anti-inflammatory activity by inhibiting LPS-induced activation of TLR4 and NOX2, which may help patients relieve the pain [ 20 ]. According to our previous research, NK enhances cell stemness and induces epithelial cell regeneration and angiogenesis, thereby promoting wound healing [ 21 ]. These results indicate that NK might be effective in oral mucositis healing.…”

Section: Introductionmentioning

confidence: 99%

Nattokinase crude extract enhances oral mucositis healing

et al. 2021

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Background Nattokinase (NK) is a promising alternative in the prevention and treatment of cardiovascular diseases due to its potent fibrinolytic activity. In this study, we investigated the effect of crude nattokinase extract on the healing of acetic acid-induced oral mucositis in mice. Methods Bacillus subtilis culture media (BSCM) was isolated into the supernatant, named nattokinase crude extract (NCE), and the pellet was named Bacillus subtilis mass (BSM). An oral mucositis model was established in mice by applying 50% glacial acetic acid to the buccal mucosa. According to the treatment conditions, the mice were divided into BSCM, NCE, BSM and phosphate buffered saline (PBS) groups. The weight of the mice, oral mucositis healing score and histopathological examination were used to evaluate the treatment. Results Fibrinolytic activities of BSCM, NCE and BSM were approximately 8069, 10,800 and 80 U/ml, respectively. The weight gain of mice in the NCE group was significantly different from the PBS group after three days’ treatment (p < 0.05). The oral mucositis score of NCE group was significantly higher than other groups (p < 0.05). The differences in histopathology scores between the NCE and other groups were statistically significant (p < 0.01). Conclusions NCE could possess remarkable potential to reduce pain and promote oral mucositis healing with minimal safety concerns. In this study, we first report that NCE from the supernatant of Bacillus subtilis can promote the healing of oral mucositis, which extends the application scope of NK.

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