Natural dyes versus lysochrome dyes in cheiloscopy: A comparative evaluation

Singh, Narendra Nath; Brave, V R; Khanna, Shally

doi:10.4103/0974-2948.71051

Cited by 20 publications

(11 citation statements)

References 3 publications

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“…(2010)[ 13 ] proved that fluorescent dyes could give results comparable with lysochrome dyes such as Sudan Black B. Singh et al . (2010),[ 14 ] conducted a similar study using Sudan Black B, on bone china cups, and satin and other cotton fabrics, from which they concluded that natural dyes such as vermilion and indigo gave results comparable to Sudan Black B in developing latent lip prints. In our study we have made use of the most efficacious lysochrome dye, Sudan black B [as per Navarro et al .…”

Section: Discussionmentioning

confidence: 99%

Assessment of cheiloscopy in sex determination using lysochrome - A preliminary study

Ramakrishnan

Bahirwani

Valambath

2015

J Forensic Dent Sci

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Introduction:The present study was undertaken with the objective of ascertaining whether latent lip prints generated by persistent lipsticks and developed using lysochrome dyes have the potential of use in sex determination and personal identification.Materials and Methods:This study included a total of 100 subjects (50 males and 50 females) whose latent lip prints were obtained by applying the persistent lipstick Revlon ColorStay Overtime® manufactured by Revlon® consumer products corporation, NewYork, USA, and lifting the prints with cellophane sheets. The prints were then developed using lysochrome dyes, and all the samples were blinded and then graded based on defined patterns from the Suzuki and Tsuchihashi classification.Results:No two lip prints were found to be alike. Type I was found to be the most prevalent type. In the female population, Type I (61%) was most prevalent, followed by Type I’ (28%), Type II (9%), Type III (2%), Type IV (1%), and Type V (1%); in the male population, Type I (33%) was most prevalent, followed by Type II (23%), Type III (18%), Type IV (14%), Type I’ (10%), and Type V (3%). Two examiners were able to determine the correct sexes from the given sample sizes. Their interobserver agreement was assessed using the kappa coefficient for males (κ =0.870) and females (κ = 0.870). Their accuracy was assessed with a confidence interval (CI) of 91.48-99.38.Conclusion:Lysochrome dyes are very efficacious in developing latent lip prints. This preliminary study has conclusively proved that latent lip prints developed with lysochrome dyes hold the potential for use in sex determination and can be maintained in a digital database.

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Section: Discussionmentioning

confidence: 99%

Assessment of cheiloscopy in sex determination using lysochrome - A preliminary study

Ramakrishnan

Bahirwani

Valambath

2015

J Forensic Dent Sci

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“…Oil Red O is a fat-soluble dye that stains neutral triglycerides and lipids [27]. Intracellular triglyceride droplets of hADMSCs were stained with Oil Red O solution on day 4, 10, and 21.…”

Section: Oil Red O Stainingmentioning

confidence: 99%

Chronic DDE Exposure Modifies Mitochondrial Respiration during Differentiation of Human Adipose-Derived Mesenchymal Stem Cells into Mature Adipocytes

et al. 2021

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The contribution of environmental pollutants to the obesity pandemic is still not yet fully recognized. Elucidating possible cellular and molecular mechanisms of their effects is of high importance. Our study aimed to evaluate the effect of chronic, 21-day-long, 2,2-bis (4-chlorophenyl)-1,1-dichlorethylenedichlorodiphenyldichloroethylene (p,p′-DDE) exposure of human adipose-derived mesenchymal stem cells committed to adipogenesis on mitochondrial oxygen consumption on days 4, 10, and 21. In addition, the mitochondrial membrane potential (MMP), the quality of the mitochondrial network, and lipid accumulation in maturing cells were evaluated. Compared to control differentiating adipocytes, exposure to p,p′-DDE at 1 μM concentration significantly increased basal (routine) mitochondrial respiration, ATP-linked oxygen consumption and MMP of intact cells on day 21 of adipogenesis. In contrast, higher pollutant concentration seemed to slow down the gradual increase in ATP-linked oxygen consumption typical for normal adipogenesis. Organochlorine p,p′-DDE did not alter citrate synthase activity. In conclusion, in vitro 1 μM p,p′-DDE corresponding to human exposure is able to increase the mitochondrial respiration per individual mitochondrion at the end of adipocyte maturation. Our data reveal that long-lasting exposure to p,p′-DDE could interfere with the metabolic programming of mature adipocytes.

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“…Synthetic substances such as aluminum powder, silver metallic or lead carbonate powder have also been used to develop latent lip prints 4 . The lysosome dyes (eg, Sudan III, Sudan Black and Oil Red O) dissolve in lipids produced from the sebaceous glands of the lips 6 . These dyes effective stain both non‐porous and porous surfaces 6 …”

Section: Introductionmentioning

confidence: 99%

“…The lysosome dyes (eg, Sudan III, Sudan Black and Oil Red O) dissolve in lipids produced from the sebaceous glands of the lips 6 . These dyes effective stain both non‐porous and porous surfaces 6 …”

Section: Introductionmentioning

confidence: 99%

Vaseline treatment combined with a natural dye extract to enhance lip print contrast images

Mebuathong

Kangsanan

Chingthongkham

et al. 2021

Coloration Technology

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Fingerprinting and DNA comparisons are the most commonly used methods in criminal investigations. However, lip prints can also be used as a supplementary tool because of the uniqueness of morphological patterns. A lip print not only can be used for personal screening by its pattern, it also carries genetic information that enables personal identification. Nowadays, there are various kinds of plants that are used for biological and histological staining due to an increasing market value associated with their application and sustainability. This study assessed the ability of natural dye extract to develop latent lip print contrast images and to preserve DNA for short tandem repeat analysis. Results revealed that roselle extract showed potential for the development of vaseline‐treated lip print contrast images on bond paper. Moreover, when roselle extract‐stained paper was compared with unstained paper, short tandem repeat profiles were not affected. Therefore, such dyes could be utilised for the development of latent lip print contrast images and their application could be extensively used to establish a personal identification database for reference in criminal investigations.

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Natural dyes versus lysochrome dyes in cheiloscopy: A comparative evaluation

Cited by 20 publications

References 3 publications

Assessment of cheiloscopy in sex determination using lysochrome - A preliminary study

Assessment of cheiloscopy in sex determination using lysochrome - A preliminary study

Chronic DDE Exposure Modifies Mitochondrial Respiration during Differentiation of Human Adipose-Derived Mesenchymal Stem Cells into Mature Adipocytes

Vaseline treatment combined with a natural dye extract to enhance lip print contrast images

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