Nature of the Vehicle Solution for Cryopreservation of Human Peripheral Veins: Preservation of Reactivity to Pharmacological Stimuli

Schilling, A; Glusa, Erika; Müller‐Schweinitzer, Else

doi:10.1006/cryo.1995.1010

Cited by 15 publications

(5 citation statements)

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“…23 Indeed, experimental evidence suggests that for many cell types, e.g., for endothelial cells in canine coronary arteries, it seems to be important to have at least 20% serum in the cryomedium. 24 However, comparative studies on various vascular tissues revealed that neither rabbit carotid arteries 25 nor human saphenous veins, 6,26 human coronary and mesenteric arteries 27 require FCS for optimal preservation of smooth muscle and post-thaw endothelial function.…”

Section: Introductionmentioning

confidence: 99%

Cryopreservation of vascular tissues

Müller‐Schweinitzer¹

2009

Organogenesis

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Cryopreservation of human blood vessels may become an important tool in bypass surgery and peripheral vascular reconstruction. Ideally cryopreservation of a blood vessel should preserve functional characteristics comparable to those of fresh controls. The key advantage of cryopreservation is the fact that storage at deep subzero temperatures allows storage of structurally intact living vascular tissues for virtually infinite time. Originally developed for long-time storage of isolated cells, the techniques of cryopreservation of tissues are challenged by the fact that these are complex multicellular systems containing diverse types of cells with differing requirements for optimal preservation. Therefore, the post-thaw functional activity of vascular tissues is determined by the type of blood vessel and, in addition, by the cell packing effect. Moreover, evidence from pharmacological studies suggests that cryopreservation induces tissue specific changes in transmembrane signaling and the mechanisms coupling intracellular calcium release, sensitivity and calcium entry into the smooth muscle cells.

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Section: Introductionmentioning

confidence: 99%

Cryopreservation of vascular tissues

Müller‐Schweinitzer¹

2009

Organogenesis

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“…30 In most studies on the cryopreservation of blood vessels, DMSO was used at concentrations in the narrow range of 11% to 16%, ie, 1.4 to 2.0 mmol/L, and the equilibration times of the tissues in DMSO varied from 10 to 60 minutes. 5,7,15,20,29 With respect to the equilibration times in DMSO, optimal post-thaw functional recovery in terms of contractions and endothelium-independent relaxations was obtained with canine femoral arteries that had been frozen within 10 to 20 minutes of being placed in the cryomedium. 21 Retention of endothelium function in human saphenous vein 5,29 and canine coronary arteries 20 following cryoconservation has been demonstrated after equilibration times in DMSO of 10 or 30 minutes.…”

Section: Discussionmentioning

confidence: 99%

“…The reactivity of human saphenous veins has been extensively studied on both unfrozen and cryopreserved tissues. [4][5][6][7][8] However, no data exists concerning the in vitro reactivity of HFAs, whereas the arteries appear considerably more susceptible to cryoinjury than the veins. 6 The responses to various contracting and relaxing agents of both unfrozen HFAs and HFAs cryopreserved for 7 days and 30 days was assessed.…”

Section: Introductionmentioning

confidence: 99%

Functional assessment of human femoral arteries after cryopreservation

Stanke¹,

Riebel

Carmine

et al. 1998

Journal of Vascular Surgery

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The cryopreservation method used provided a limited preservation of HFAs contractility, a good preservation of the endothelium-independent relaxant responses, but no apparent preservation of the endothelium-dependent relaxation. It is possible that further refinements of the cryopreservation protocol, such as a slower rate of cooling and a more controlled stepwise addition of dimethyl sulfoxide, might allow better post-thaw functional recovery of HFAs.

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“…57 All studies agree that recuperation of contraction of smooth muscle fibers upon stimulation with noradrenaline, 5-hyrosytryptamine, norepinephrine and thromboxane is no greater than 30-40%, while the relaxation response to acetylcholine, papaverin and bimakalin is conserved. 55,58,59 Protocols for cyropreservation of vascular segments, as for all others tissues, use antibiotics. Inclusion of imipenem and fluctosine 60 during incubation at 37°C or cryopreservation is without risk for endothelial cell survival.…”

Section: Cryopreservation Of Cartilagementioning

confidence: 99%