The cryopreservation of composite tissues

Bakhach, Joseph

doi:10.4161/org.5.3.9583

Cited by 120 publications

(48 citation statements)

References 82 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…As such, tissues are composed of a variety of cell types with varying degree of vulnerability to the cryopreservation process. 30 While the feasibility of cryopreservation is well established, several cryoprotectant agents are utilized, either lab-based or commercially available, for varying applications. As a result, the optimal method of cryopreservation of PDX tissue remains to be elucidated and given the significant amount of resources required for generation of a single patient PDX model, any loss of such would have devastating consequences on the viability of any program.…”

Section: Introductionmentioning

confidence: 99%

Patient-derived xenograft cryopreservation and reanimation outcomes are dependent on cryoprotectant type

Ivanics

Bergquist

Liu

et al. 2018

Laboratory Investigation

View full text Add to dashboard Cite

Introduction Patient-derived xenografts (PDX) are being increasingly utilized in preclinical oncologic research. Maintaining large colonies of early generation tumor-bearing mice is impractical and cost-prohibitive. Optimal methods for efficient long-term cryopreservation and subsequent reanimation of PDX tumors are critical to any viable PDX program. We sought to compare the performance of “Standard” and “Specialized” cryoprotectant media on various cryopreservation and reanimation outcomes in PDX tumors. Standard (10% DMSO media) and Specialized (Cryostor®) media were compared between overall and matched PDX tumors. Primary outcome was reanimation engraftment efficiency (REE). Secondary outcomes included time-to-tumor-formation (TTF), time-to-harvest (TTH), and potential loss of unique PDX lines. Overall 57 unique PDX tumors underwent 484 reanimation engraftment attempts after previous cryopreservation. There were 10 unique PDX tumors cryopreserved with Standard (71 attempts), 40 with Specialized (272 attempts), and 7 with both (141 attempts). Median frozen time of reanimated tumors was 29 weeks (max.177). Tumor pathology, original primary PDX growth rates, frozen storage times, and number of implantations per PDX model were similar between cryoprotectant groups. Specialized media resulted in superior REE (overall: 82% vs. 39%, p<0.0001; matched: 97% vs. 36%, p<0.0001; >52 weeks cryostorage: 59% vs. 9%, p<0.0001), shorter TTF (overall 24 vs. 54 days, p=0.0051; matched 18 vs. 53 days, p=0.0013) and shorter TTH (overall: 64 vs. 89 days, p=0.009; matched: 47 vs. 88 days, p=0.0005) compared to Standard. Specialized media demonstrated improved REE with extended duration cryostorage (p=0.048) compared to Standard. Potential loss of unique PDX lines was lower with Specialized media (9% vs. 35%, p=0.017). In conclusion, cryopreservation with a specialized cryoprotectant appears superior to traditional laboratory-based media and can be performed with reliable reanimation even after extended cryostorage.

show abstract

Section: Introductionmentioning

confidence: 99%

Patient-derived xenograft cryopreservation and reanimation outcomes are dependent on cryoprotectant type

Ivanics

Bergquist

Liu

et al. 2018

Laboratory Investigation

View full text Add to dashboard Cite

show abstract

“…Cryopreservation is distinguished from freezing in that cryoprotective agents are added to preserve the biological integrity of the tissues [2,3]. Freezing of tissue, such as bone, is done without the use of cryoprotective agents and usually the temperature is not less than -80°C.…”

Section: Introductionmentioning

confidence: 99%

Cryopreservation and replantation of amputated rat hind limbs

Wang

Duan

et al. 2014

Eur J Med Res

View full text Add to dashboard Cite

BackgroundIn spite of the relatively high success rate of limb replantation, many patients cannot undergo replantation surgery because the preservation time of an amputated limb is only about six hours. In addition, although allotransplantation of composite tissues is being performed more commonly with increasingly greater success rates, the shortage of donors limits the number of patients that can be treated. So the purpose of this study is to examine the feasibility of cryopreservation and replantation of limbs in a rat model.MethodsTwelve five-month-old Sprague-Dawley rats were divided evenly into group A (above-knee amputation) and group B (Syme’s amputation). One hind limb was amputated from each rat. The limbs were irrigated with cryoprotectant, cooled in a controlled manner to -140°C, and placed in liquid nitrogen. Thawing and replantation were performed 14 days later.ResultsIn group A, the limbs became swollen after restoration of blood flow resulting in blood vessel compression and all replantations failed. In group B, restoration of blood flow was noted in all limbs after replantation. In one case, the rat chewed the replanted limb and replantation failed. The other five rats were followed for three months with no abnormalities noted in the replanted limbs.ConclusionsLimbs with a minimal amount of muscle tissue can be successfully cryopreserved and replanted.

show abstract

“…The low temperatures allow the cells to enter a quiescent state in which cellular functions are suspended without affecting their intrinsic characteristics (1). Peripheral blood mononuclear cells (PBMCs) are frequently cryopreserved for use in transplants or immunological studies (2,3).…”

mentioning

confidence: 99%

Viability and Functionality of Cryopreserved Peripheral Blood Mononuclear Cells in Pediatric Dengue

Perdomo‐Celis

Salgado

Castañeda

et al. 2016

Clin Vaccine Immunol

View full text Add to dashboard Cite

bCryopreserved peripheral blood mononuclear cells (PBMCs) are widely used in studies of dengue. In this disease, elevated frequency of apoptotic PBMCs has been described, and molecules such as soluble tumor necrosis factor (TNF)-related apoptosisinducing ligands (sTRAIL) are involved. This effect of dengue may affect the efficiency of PBMC cryopreservation. Here, we evaluate the viability (trypan blue dye exclusion and amine-reactive dye staining) and functionality (frequency of gamma interferon [IFN-␥]-producing T cells after polyclonal stimulation) of fresh and cryopreserved PBMCs from children with dengue (in acute and convalescence phases), children with other febrile illnesses, and healthy children as controls. Plasma sTRAIL levels were also evaluated. The frequencies of nonviable PBMCs detected by the two viability assays were positively correlated (r ‫؍‬ 0.74; P < 0.0001). Cryopreservation particularly affected the PBMCs of children with dengue, who had a higher frequency of nonviable cells than healthy children and children with other febrile illnesses (P < 0.02), and PBMC viability levels were restored in the convalescent phase. In the acute phase, an increased frequency of CD3 ؉ CD8 ؉ amine-positive cells was found before cryopreservation (P ‫؍‬ 0.01). Except for B cells in the acute phase, cryopreservation usually did not affect the relative frequencies of viable PBMC subpopulations. Dengue infection reduced the frequency of IFN-␥-producing CD3؉ cells after stimulation compared with healthy controls and convalescent-phase patients (P < 0.003), and plasma sTRAIL correlated with this decreased frequency in dengue (rho ‫؍‬ ؊0.56; P ‫؍‬ 0.01). Natural dengue infection in children can affect the viability and functionality of cryopreserved PBMCs. Cryopreservation is the maintenance of cells and biological tissues at low temperatures and is based on the use of various media or solutions that form hydrogen bonds with water molecules, preventing cellular damage. The low temperatures allow the cells to enter a quiescent state in which cellular functions are suspended without affecting their intrinsic characteristics (1). Peripheral blood mononuclear cells (PBMCs) are frequently cryopreserved for use in transplants or immunological studies (2, 3). However, the cryopreservation process may affect viability, phenotype, and cellular functionality due to factors such as inadequate temperatures, the freezing protocol used, the expertise of the personnel, and freezing time (4, 5). The disease of the individual from whom the PBMCs come also affects cryopreservation. For example, PBMCs from subjects infected with human immunodeficiency virus (HIV) presented reduced viability after cryopreservation, possibly due to the increased numbers of apoptotic cells circulating during the course of the disease (6). Similar findings have been found in the acute phases of diseases, such as visceral leishmaniasis (7). Particularly for HIV, great efforts have been undertaken to optimize the evaluation and comparability of immune tests...

show abstract

The cryopreservation of composite tissues

Cited by 120 publications

References 82 publications

Patient-derived xenograft cryopreservation and reanimation outcomes are dependent on cryoprotectant type

Patient-derived xenograft cryopreservation and reanimation outcomes are dependent on cryoprotectant type

Cryopreservation and replantation of amputated rat hind limbs

Viability and Functionality of Cryopreserved Peripheral Blood Mononuclear Cells in Pediatric Dengue

Contact Info

Product

Resources

About