ncRNAs regulate bovine adipose tissue deposition

Lei, Zhaoxiong; Wu, Huiguang; Xiong, Yan; Wei, Dawei; Wang, Xingping; Luoreng, Zhuoma; Cai, Xiaoyan; Ma, Yun

doi:10.1007/s11010-021-04132-2

Cited by 8 publications

(4 citation statements)

References 67 publications

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“…However, an increasing number of noncoding RNAs (ncRNAs), including microRNAs (miRNAs) [ 8 – 10 ], long noncoding RNAs (lncRNAs) [ 11 – 14 ], and circular RNAs (circRNAs) [ 15 , 16 ], have been shown to be involved in the regulation of adipocyte metabolism [ 17 ]. These ncRNAs regulate adipogenesis in a variety of ways [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Emerging functions of circular RNA in the regulation of adipocyte metabolism and obesity

Zhang

Tian

et al. 2022

Cell Death Discov.

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As noncoding RNAs, circular RNAs (circRNAs) are covalently enclosed endogenous biomolecules in eukaryotes that have tissue specificity and cell specificity. circRNAs were once considered a rare splicing byproduct. With the development of high-throughput sequencing, it has been confirmed that they are expressed in thousands of mammalian genes. To date, only a few circRNA functions and regulatory mechanisms have been verified. Adipose is the main tissue for body energy storage and energy supply. Adipocyte metabolism is a physiological process involving a series of genes and affects biological activities in the body, such as energy metabolism, immunity, and signal transmission. When adipocyte formation is dysregulated, it will cause a series of diseases, such as atherosclerosis, obesity, fatty liver, and diabetes. In recent years, many noncoding RNAs involved in adipocyte metabolism have been revealed. This review provides a comprehensive overview of the basic structure and biosynthetic mechanism of circRNAs, and further discusses the circRNAs related to adipocyte formation in adipose tissue and liver. Our review will provide a reference for further elucidating the genetic regulation mechanism of circRNAs involved in adipocyte metabolism.

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Section: Introductionmentioning

confidence: 99%

Emerging functions of circular RNA in the regulation of adipocyte metabolism and obesity

Zhang

Tian

et al. 2022

Cell Death Discov.

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“…The miRNAs could be used to find their lncRNAs target; action of miRNAs on lncRNAs could be sequestering them by binding or destroying them by cleaving ( Assmann et al, 2019 ; Xie et al, 2020 ). The tissue-wise extracted circRNAs in the present study could be utilized in the studies of tissue-specific post-transcriptional regulation involving circRNAs and their role in various buffalo diseases ( Gao et al, 2018 ; He et al, 2021 ; Lei et al, 2021 ; Yang et al, 2021 ).…”

Section: Resultsmentioning

confidence: 99%

“…The functions of circRNAs are not well known but still it is reported that they play a significant role in post-transcriptional regulation of gene expression ( Lukiw, 2013 ). CircRNAs function as a sponge of miRNAs by sequestering them by binding and interacting with lncRNAs ( Lei et al, 2021 ). These are being employed as biomarkers for controlling and treating diseases ( Meng et al, 2017 ; Lu, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Whole-Genome-Based Web Genomic Resource for Water Buffalo (Bubalus bubalis)

et al. 2022

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Water buffalo (Bubalus bubalis), belonging to the Bovidae family, is an economically important animal as it is the major source of milk, meat, and drought in numerous countries. It is mainly distributed in tropical and subtropical regions with a global population of approximately 202 million. The advent of low cost and rapid sequencing technologies has opened a new vista for global buffalo researchers. In this study, we utilized the genomic data of five commercially important buffalo breeds, distributed globally, namely, Mediterranean, Egyptian, Bangladesh, Jaffrarabadi, and Murrah. Since there is no whole-genome sequence analysis of these five distinct buffalo breeds, which represent a highly diverse ecosystem, we made an attempt for the same. We report the first comprehensive, holistic, and user-friendly web genomic resource of buffalo (BuffGR) accessible at http://backlin.cabgrid.res.in/buffgr/, that catalogues 6028881 SNPs and 613403 InDels extracted from a set of 31 buffalo tissues. We found a total of 7727122 SNPs and 634124 InDels distributed in four breeds of buffalo (Murrah, Bangladesh, Jaffarabadi, and Egyptian) with reference to the Mediterranean breed. It also houses 4504691 SSR markers from all the breeds along with 1458 unique circRNAs, 37712 lncRNAs, and 938 miRNAs. This comprehensive web resource can be widely used by buffalo researchers across the globe for use of markers in marker trait association, genetic diversity among the different breeds of buffalo, use of ncRNAs as regulatory molecules, post-transcriptional regulations, and role in various diseases/stresses. These SNPs and InDelscan also be used as biomarkers to address adulteration and traceability. This resource can also be useful in buffalo improvement programs and disease/breed management.

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“…For example, sterol regulatory element binding protein (SREBP) transcription factor (13), phosphoenolpyruvate carboxykinase1 (PCK1) (3,14), and fatty acid binding protein (FABP4) (9). In addition, non-coding RNAs (miRNAs, lncRNAs, and circRNAs) also play an important role in regulating the economic traits of livestock and poultry, and they interact with coding RNAs to form a regulatory network to jointly regulate fat deposition (15). circRNAs, a type of endogenous non-coding RNA with covalently closed loop structure (16,17), have become a research hotspot in recent years.…”

Section: Introductionmentioning

confidence: 99%

Weighted Gene Co-expression Network Analysis Revealed That CircMARK3 Is a Potential CircRNA Affects Fat Deposition in Buffalo

Xue

Jian-Gao

et al. 2022

Front. Vet. Sci.

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BackgroundBuffalo meat is increasingly widely accepted for consumption as it shares several quality attributes with cattle meat (beef). Hence, there is a huge opportunity for growth in the buffalo meat industry. However, buffalo meat has relatively low intramuscular fat (IMF) content, affecting its flavor, tenderness and juiciness. As there is a dearth of information on factors that control fat deposition, this study was undertaken to provide new candidate factor associated with buffalo fat deposition. Circular RNA (circRNA) is a novel class of non-coding RNA with a closed-loop structure, and play an important role in fat deposition.MethodsIn this study, weighted gene co-expression network analysis (WGCNA) was used to construct a circRNA co-expression network and revealed a candidate circRNA that may affect the IMF deposition of buffalo as determined by RT-qPCR, semiquantitative PCR and gain-of-function experiments.ResultsHerein, WGCNA determined that one module (turquoise module) is significantly associated with the growth and development stages of buffalo. Further analysis revealed a total of 191 overlapping circRNAs among differentially expressed (DE) circRNAs and the co-expression module. A candidate circRNA was found, 21:6969877|69753491 (circRNA_ID), with a reported involvement in lipid metabolism. This circRNA is stably expressed and originates from the MARK3 gene, hence the name circMARK3. circMARK3 is highly expressed in adipose tissue and mature adipocytes and is located in the cytoplasm. Gain-of-function experiments demonstrated that circMARK3 promoted adipogenic differentiation of buffalo adipocytes and 3T3-L1 cells by up-regulating the expression levels of adipogenic marker genes PPARG, C/EBPα and FABP4.ConclusionThese results indicate that circMARK3 is a potential factor that promotes fat deposition by regulating adipocyte differentiation and adipogenesis in buffalo.

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ncRNAs regulate bovine adipose tissue deposition

Cited by 8 publications

References 67 publications

Emerging functions of circular RNA in the regulation of adipocyte metabolism and obesity

Emerging functions of circular RNA in the regulation of adipocyte metabolism and obesity

Whole-Genome-Based Web Genomic Resource for Water Buffalo (Bubalus bubalis)

Weighted Gene Co-expression Network Analysis Revealed That CircMARK3 Is a Potential CircRNA Affects Fat Deposition in Buffalo

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