NDM-1-Producing Citrobacter freundii, Escherichia coli, and Acinetobacter baumannii Identified from a Single Patient in China

Abstract

eWe identified New Delhi metallo-␤-lactamase (NDM-1)-producing Citrobacter freundii GB032, Escherichia coli GB102, and Acinetobacter baumannii GB661 in urine and stool samples from a single patient in China. Plasmid profiling and Southern blotting indicated that bla NDM-1 from GB032 and that from GB102 were likely located on the same plasmid, while bla NDM-1 from GB661 was located on a very large (>400-kb) plasmid. This case underscores the broad host range of bla NDM-1 and its potential to spread between members of the family Enterobacteriaceae and A. baumannii.

Insights on the Horizontal Gene Transfer of Carbapenemase Determinants in the Opportunistic Pathogen Acinetobacter baumannii

“…Interestingly, a clinical case in China reports the finding of bla NDM-1 in E. coli , Citrobacter freundii and A. baumannii collected from the same patient but while bla NDM-1 was inserted in an identical plasmid in the Enterobacteria, in A. baumannii it was found to be located in a very large plasmid (>400-Kb) [ 104 ]. The genetic environment was not analysed.…”

Genomic Characterization of A Multidrug-Resistant Citrobacter Freundii Strain ZT01-0079 Co-Producing blaNDM-1 and blaSHV-12 Using MiSeq and MinION

“…In recent years, due to the abuse of antibiotics, C. freundii has acquired increasing resistance to common antibiotics. In addition, bla NDM−1 -positive C. freundii has been reported in numerous countries that include but are not limited to China [2][3][4][5], India, Pakistan, France, Sweden, Australia, UK, USA [7], and South Africa [9]. We report a carbapenem-resistant strain of C. freundii with coexistent bla NDM−1 and bla SHV−12 genes on a transferrable IncX3 plasmid.…”

“…New Delhi metallo-β-lactamase-1 (NDM-1) is an enzyme capable of hydrolyzing most β-lactam antibiotics. Since its rst detection in 2009 in Klebsiella pneumoniae [1], 21 NDM variants have been reported from various Enterobacteriaceae species [2][3][4][5][6][7][8][9][10][11][12][13][14][15]. The increasing prevalence of NDM-producing bacteria is due primarily to intra-and inter-species exchanges of a variety of self-transferring plasmids.…”

Carbapenem Resistance in Clonally Distinct Clinical Strains ofVibrio fluvialisIsolated from Diarrheal Samples

“…Even though we demonstrated the in vitro transfer of bla NDM-1 in other enteric bacteria, these bacteria are not completely resistant to carbapenems, as is V. fluvialis . Multiple NDM-1–producing pathogens belonging to different species from a patient have been reported ( 31 ). Although in our study, 14 of 27 patients were infected with other pathogens (enteroaggressive E. coli [EAEC], enterotoxigenic E .…”