Fortification structures, both military and civilian, are designed to resist a blast explosion to some extent. Their technical condition after a blast load must be assessed in a fast and reliable way to enable the users’ decision about the future use of the structure. Preferably, for the assessment of the protective structure, the non-destructive testing method should be used. To assess the suitability of ultrasound testing method for fortification structures built from steel fiber reinforced concrete, an investigation in a laboratory and in situ was conducted, together with numerical simulation and statistical evaluation. The numerical simulation of the blast load of a steel fiber reinforced concrete slab was conducted using multiphysics simulation software with the aim to verify basic parameters of the field experiment. During the field tests, several slabs were loaded by plastic explosive and changes in the structure of the slabs, before and after the blast load, were examined using the ultrasound pass-through method. After the field tests, the slabs were subjected to a destructive laboratory test to determine their residual strength. Subsequently, the data sets obtained from the measurements were tested using functional data analysis. The results from the ultrasound pulse method show that specimens after a dynamic blast load can in some cases increase the strength of their cement matrix.