Near-infrared Au nanorods in photodynamic therapy, hyperthermia agents, and near-infrared optical imaging

Effect of Size-Dependent Photodestructive Efficacy by Gold Nanomaterials with Multiphoton Laser

“…After incubation, the number of bacteria surviving by colony forming unit (CFU mL −1 ) counting assay was determined. 2,7 For A549 Cells. First, 5 × 10 3 cells per well in a 96-well culture plate were for overnight of incubation in the dark at 37°C with 5% CO 2 in air.…”

“…The protocol to culture A549 cells followed previous studies. 1,7 Antibodies Conjugation. For the improvement of specificity and therapeutic efficiency, ICG and all Au NPs and Au−PEI−ICG NPs were coated with the antiprotein A antibodies and antiepidermal growth factor receptor (EGFR) antibodies (Antagene, Inc., Santa Clara, CA), respectively.…”

“…The MRSA was respectively added to the ICG−Ab protein A , Au NP−Ab protein A , and Au−PEI−ICG NP−Ab protein A for 6 h of incubation in the dark at 37°C, whereas the cell monolayer with A549 was respectively immersed with the ICG−Ab EGFR , Au NP− Ab EGFR , and Au−PEI−ICG NP−Ab EGFR for 2 h, and then continued to go on the following experiment. 2,7 Cytotoxicity Assay. For MRSA.…”

“…Transmission electron microscopes (TEM) (JEOL 1400 and JEOL 2100, Japan) were used to characterize the morphology and energy dispersive X-ray (EDX) of the samples. 7 The ultraviolet−visible spectroscopy (UV−vis) absorption spectrometer (U-4100, Hitachi, Japan), Fourier transform infrared (FTIR) spectrometer (PerkinElmer RX1, USA), zeta potential spectrometer spectra (Manern Nano-ZS90, UK), and fluorescence spectrometer (FP-6600, JASCO) 2 were also used to determine the characters of samples.…”

“…2,6 Indocyanine green (ICG) is an anionic photosensitizer that absorbs in NIR window and is used in photodynamic therapy (PDT), which produces reactive oxygen species (ROS) to destroy and eliminate cancer cells and tissues via its photosensitizing property. 2,7 ICG has been approved by the U.S. Food and Drug Administration for medical diagnostic studies, 2 and it acts as a fluorescent contrast agent for enzymes and proteins and produces singlet oxygen, a type of ROS, that destroys cancer cells and tumors. Studies have reported that ICG is used to weld tissue with an NIR laser and provides a photothermal effect in clinical therapy.…”