Near-infrared emission in the catalase-hydrogen peroxide system: a reevaluation

Kanofsky, Jeffrey R.; Hines, Edward R.

doi:10.1021/ja00327a042

Cited by 15 publications

(6 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Scan rate: 600 nm in 180 s. In B the thermal emission from the heated solvent (giving an apparent maximum at ca. 1600 nm due to the drop in detector sensitivity) is seen in addition to the '02 peak (Kanofsky, 1984). Figure 2B shows two scans, one taken immediately after addition of the peroxide, the other after its complete decomposition.…”

Section: Resultsmentioning

confidence: 99%

Spectral Observation of Singlet Molecular Oxygen From Aromatic Endoperoxides in Solution

Wilson

Khan

Mehrotra

1986

Photochem & Photobiology

View full text Add to dashboard Cite

show abstract

Section: Resultsmentioning

confidence: 99%

Spectral Observation of Singlet Molecular Oxygen From Aromatic Endoperoxides in Solution

Wilson

Khan

Mehrotra

1986

Photochem & Photobiology

View full text Add to dashboard Cite

show abstract

“…Singlet oxygen, which is a strong electrophile but not a radical, can oxidize TEMP (a sterically hindered amine) to a stable N-oxyl radical, TEMPO (Aurich 1982), which can be detected by EPR spectroscopy (Lion et al 1976(Lion et al , 1980. Also, in the absence of reactions 10 2 returns to the ground state with light emission around 1270 nm as spontaneous monomolar decay (Kanofsky 1983(Kanofsky , 1984. This infrared chemiluminescence was recently applied for the detection of 10 2 from photodamaged PS II reaction centre preparations (Teller et al 1992, Macpherson et al 1993.…”

Section: Introductionmentioning

confidence: 99%

Singlet oxygen production in thylakoid membranes during photoinhibition as detected by EPR spectroscopy

1994

View full text Add to dashboard Cite

Exposure of isolated spinach thylakoids to high intensity illumination (photoinhibition) results in the well-characterized impairment of Photosystem II electron transport, followed by degradation of the D1 reaction centre protein. In the present study we demonstrate that this process is accompanied by singlet oxygen production. Singlet oxygen was detected by EPR spectroscopy, following the formation of stable nitroxide radicals from the trapping of singlet oxygen with a sterically hindered amine TEMP (2,2,6,6-tetramethylpiperidine). There was no detectable singlet oxygen production during anaerob photoinhibition or in the presence of sodium-azide. Comparing the kinetics of the loss of PS II function and D1 protein with that of singlet oxygen trapping suggests that singlet oxygen itself or its radical product initiates the degradation of D1.

show abstract

“…[For an alternative view see(171)]. These experimental observations were possible because of the recent de velopment of sensitive infrared spectroscopic techniques to detect 102 in solution(154,159), and are understood as the logical consequence following the original discovery of 102 generation by Khan & Kasha in 1 963(172).…”

mentioning

confidence: 99%