Need, Strategies and Requirements in the Medical System for Bone Banks: A Review Article

Verma, Priyanshu R; Anjankar, Ashish; Singh, Parth V

doi:10.7759/cureus.28785

Cited by 3 publications

(1 citation statement)

References 31 publications

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“…У 1881 році Macewen вперше описав використання алогенної кісткової тканини людини. Відтоді трансплантація алогенної кістки стає все поширенішою і нині є однією з провідних форм ортопедичного лікування [4]. Хірурги все частіше обирають алотрансплантат внаслідок більшої доступності, відомої остеокондуктивності та через відсутність пошкоджень донорської ділянки.…”

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Serum biochemical indicators in rats of different ages after replacing femoral metaphysis defects with allogeneic bone implants saturated with mesenchymal stem cells

Vorontsov,

Korzh,

Leontieva

et al. 2023

ZMJ

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The aim of the study: to evaluate the course of metabolic processes after replacing femoral metaphysis defects with allogeneic bone implants saturated with allogeneic mesenchymal stem cells (MSCs) based on the analysis of serum biochemical indicators of connective tissue metabolism in laboratory rats. Material and methods. A critical-sized transcortical femoral defect model in the femur metaphysis of white rats was used. Blood serum concentrations of glycoproteins, total chondroitin sulfates, total protein and calcium, alkaline and acid phosphatase activity were measured. Results. On the 28th day, in 3-month-old animals with MSC, in comparison with the data of rats without MSC, clear signs of connective tissue formation and bone resorption activity were observed, as evidenced by an increase in serum chondroitin sulfates and acid phosphatase activity. On the 90th day, this group of rats under the influence of MSCs showed signs of decreased bone tissue formation with lower serum activity of alkaline phosphatase. In 3-month-old rats with MSC, signs of the inflammatory process chronization with higher serum concentrations of glycoproteins were detected on the 90th day of the experiment in comparison with data from animals without MSC. In 12-month-old rats with MSCs, signs of bone resorption were documented on the 14th day, which were manifested by a higher serum activity of acid phosphatase with less formation of connective tissue and lower concentrations of chondroitin sulfates. On the 28th day, the effect of MSCs in 12-month-old animals was manifested in the form of a slowdown in bone formation with a decrease in alkaline phosphatase activity. On the 90th day, the connective tissue formation was activated with higher concentrations of chondroitin sulfates in these animals. Conclusions. The studied biochemical indicators in 3-month-old animals showed greater lability. The regeneration phases were faster in 3-month-old animals as well as in animals with alloimplants without MSCs. Injections of allogeneic MSCs together with an allograft immediately after a bone damage, regardless of age, caused signs of bone formation slowing and excessive formation of connective tissue, therefore, the combination of allogeneic MSCs with an allogeneic bone implant is not advisable to use in fresh fractures.

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Serum biochemical indicators in rats of different ages after replacing femoral metaphysis defects with allogeneic bone implants saturated with mesenchymal stem cells

Vorontsov,

Korzh,

Leontieva

et al. 2023

ZMJ

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Histo-morphometric evaluation of post-augmentation bone tissue of the human mandible

Oshurko,

Oliinyk,

Мaystruk

et al. 2024

Rep. of Morph.

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The clinical challenge of today is to restore the biological structure of atrophied bone tissue by combining methods of complex clinical rehabilitation, that is, intraosseous implantation and augmentation, in its interimplant sites. The aim of the study was to perform a histo-morphometric assessment of post-augmentation bone tissue using a combined technique of controlled autocellular transplantation with the use of bone allograft filler on edentulous distal segments of the human mandible with varying degrees and forms of atrophy. Trepanbiopsy specimens were used as the study material, after microtomy processing of which histological slides were prepared, followed by the accelerated May-Grunwald staining-fixation technique (Sigma-Aldrich, St. Louis, USA). For the histomorphometric study, stained histological specimens of post-augmentation bone tissue were imaged with an optical microscope camera (Leica DMLB, Germany). The analysis was performed using the Fiji information software, with the formation of reconstructed mosaic digital micrographs for further histomorphometry. The results of the evaluation of controlled bone tissue formation (post-augmentation), which are the key tasks, novelty, and justification of modern and effective methods of rehabilitation of patients with acquired forms of atrophy in the edentulous distal segments of the human mandible, are illustrated by microphotographs and presented in detail in this paper by the percentage of the quality of cross-linking of the cortical layer. The ratio of the newly formed cortical layer of bone tissue to the total area of the post-augmentation tissue study was 61.30 %, with a lateral form of atrophy (according to J. Cawood and R. Howell: class IV), in the post-augmentation period – 4 months. The histo-morphometric assessment of the qualitatively formed cortical bone in the post-augmentation period of six months is 92.80 % of the total postoperative area. Therefore, it is biased to evaluate the quality of the formed bone in the mandible within the generally accepted average period of four months, although paraclinical densitometry shows positive values.

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Efficacy and Safety of Stem Cell Therapy for Orthopedic Conditions, Including Osteoarthritis and Bone Defects

Raza,

Hassan,

Hashmi

et al. 2024

Cureus

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Introduction: Orthopedic conditions like osteoarthritis and bone defects pose significant challenges due to their impact on individuals' quality of life. Traditional treatments often provide only symptomatic relief, necessitating alternative therapies for long-term management. Stem cell therapy has grabbed attention for its regenerative and immunomodulatory properties, offering potential for tissue repair and functional restoration. Objective: This study aims to assess the efficacy and safety of stem cell therapy for orthopedic conditions, specifically osteoarthritis and bone defects. Materials and methods: A retrospective cross-sectional study analyzed data from patients who underwent stem cell therapy for osteoarthritis or bone defects between January and September 2023. Outcome measures focused on pain and function improvements using tools such as Visual Analog Scale (VAS) and Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC), alongside radiographic assessments. Adverse events, range of motion, quality of life, and demographic factors were also examined. Data were collected from electronic medical records while maintaining patient confidentiality. Descriptive statistics using SPSS (IBM Corp., Armonk, NY, USA) were employed to analyze patient characteristics, treatment variables, and outcomes, with statistical significance determined using Chi-square test and Independent t-test. Results: Out of 50 individuals, the majority, i.e., 35 (or 70%), were diagnosed with osteoarthritis, while the remaining 15 (30%) had bone defects. Treatment outcomes showed significant improvements in pain and function, with a decrease in mean VAS and WOMAC scores at the six-month follow-up. Seven participants (28%) reported adverse events, and two participants (8%) experienced serious adverse events. Conclusion: Stem cell therapy shows promise for treating orthopedic conditions like osteoarthritis and bone defects. While demonstrating efficacy in pain management and functional improvement, safety considerations warrant further investigation and optimization of treatment protocols. Future research should focus on refining stem cell therapy techniques and addressing safety concerns to maximize its therapeutic potential in orthopedic practice.

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Need, Strategies and Requirements in the Medical System for Bone Banks: A Review Article

Cited by 3 publications

References 31 publications

Serum biochemical indicators in rats of different ages after replacing femoral metaphysis defects with allogeneic bone implants saturated with mesenchymal stem cells

Serum biochemical indicators in rats of different ages after replacing femoral metaphysis defects with allogeneic bone implants saturated with mesenchymal stem cells

Histo-morphometric evaluation of post-augmentation bone tissue of the human mandible

Efficacy and Safety of Stem Cell Therapy for Orthopedic Conditions, Including Osteoarthritis and Bone Defects

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