Negative regulatory elements of the Saccharomyces cerevisiae PHO system: interaction between PHO80 and PHO85 proteins

Gilliquet, Véronique; Legrain, M; Berben, Gilbert; Hilger, François

doi:10.1016/0378-1119(90)90251-l

Cited by 14 publications

(10 citation statements)

References 25 publications

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“…3A). Within this region, candidate genes were considered that showed homology to yeast Pi starvation signaling genes (PHO80, PHO81, PHO85, PHO2, and PHO4) (Bajwa et al 1984;Legrain et al 1986;Sengstag and Hinnen 1987;Madden et al 1988;Gilliquet et al 1990;Creasy et al 1993) or to the recently Data represent means of at least six independent measurements. Standard deviations are indicated by bars.…”

Section: Phr1 Mutant Alleles Are Impaired In Different Pi Starvation mentioning

confidence: 99%

A conserved MYB transcription factor involved in phosphate starvation signaling both in vascular plants and in unicellular algae

Rubio¹,

Linhares²,

Solano³

et al. 2001

Genes Dev.

1,164

1,321

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Plants have evolved a number of adaptive responses to cope with growth in conditions of limited phosphate (Pi) supply involving biochemical, metabolic, and developmental changes. We prepared an EMS-mutagenized M 2 population of an Arabidopsis thaliana transgenic line harboring a reporter gene specifically responsive to Pi starvation (AtIPS1ϻGUS), and screened for mutants altered in Pi starvation regulation. One of the mutants, phr1 (phosphate starvation response 1), displayed reduced response of AtIPS1ϻGUS to Pi starvation, and also had a broad range of Pi starvation responses impaired, including the responsiveness of various other Pi starvation-induced genes and metabolic responses, such as the increase in anthocyanin accumulation. PHR1 was positionally cloned and shown be related to the PHOSPHORUS STARVATION RESPONSE 1 (PSR1) gene from Chlamydomonas reinhardtii. A GFPϻPHR1 protein fusion was localized in the nucleus independently of Pi status, as is the case for PSR1. PHR1 is expressed in Pi sufficient conditions and, in contrast to PSR1, is only weakly responsive to Pi starvation. PHR1, PSR1, and other members of the protein family share a MYB domain and a predicted coiled-coil (CC) domain, defining a subtype within the MYB superfamily, the MYB-CC family. Therefore, PHR1 was found to bind as a dimer to an imperfect palindromic sequence. PHR1-binding sequences are present in the promoter of Pi starvation-responsive structural genes, indicating that this protein acts downstream in the Pi starvation signaling pathway.

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Section: Phr1 Mutant Alleles Are Impaired In Different Pi Starvation mentioning

confidence: 99%

A conserved MYB transcription factor involved in phosphate starvation signaling both in vascular plants and in unicellular algae

Rubio¹,

Linhares²,

Solano³

et al. 2001

Genes Dev.

1,164

1,321

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“…PHO80 mutations have recessive inheritance and lead to constitutive synthesis of repressible acid phosphatases [87]. In pho80 PGC yeast strains, two types of mutations were identified: recessive with constitutive synthesis of repressible acid phosphatases and semidominant mutations, which lead to the lack of repressible acid phosphatase expression phenotype [88]. PHO80 expression is not governed by phosphate concentration, but still it does depend on Pho85p functionality.…”

Section: Phosphate Metabolismmentioning

confidence: 99%

Acid Phosphatases of Budding Yeast as a Model of Choice for Transcription Regulation Research

et al. 2011

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Acid phosphatases of budding yeast have been studied for more than forty years. This paper covers biochemical characteristics of acid phosphatases and different aspects in expression regulation of eukaryotic genes, which were researched using acid phosphatases model. A special focus is devoted to cyclin-dependent kinase Pho85p, a negative transcriptional regulator, and its role in maintaining mitochondrial genome stability and to pleiotropic effects of pho85 mutations.

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“…Especially the secreted acid phosphatase encoded by P H 0 5 and the regulation of its gene expression have been extensively studied. Transcription is activated by two DNA binding proteins, encoded by P H 0 2 and P H 0 4 (BURGLIN, 1988;OGAWA and OSHIMA, 1990), and negatively controlled by the products of the genes P H 0 8 0 and PH085 (GILLIQUET et al, 1990). The latter has been shown to encode a protein with strong similarities to a protein kinase (ToH-E et al, 1988).…”

Section: Lipids Vitamins and Inorganic Phosphatementioning

confidence: 99%

Yeasts

Heinisch¹,

Hollenberg²

2001

Biotechnology Set

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Negative regulatory elements of the Saccharomyces cerevisiae PHO system: interaction between PHO80 and PHO85 proteins

Cited by 14 publications

References 25 publications

A conserved MYB transcription factor involved in phosphate starvation signaling both in vascular plants and in unicellular algae

A conserved MYB transcription factor involved in phosphate starvation signaling both in vascular plants and in unicellular algae

Acid Phosphatases of Budding Yeast as a Model of Choice for Transcription Regulation Research

Yeasts

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