Neospora caninum, potential cause of abortions in dairy cows: The current serological follow-up in Slovakia

Reiterová, K.; Špilovská, Silvia; Antolová, Daniela; Dubinský, P.

doi:10.1016/j.vetpar.2008.10.008

Cited by 25 publications

(23 citation statements)

References 34 publications

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“…The seroprevalence (27.7%) in 3 counties (Arad, Bihor, and Timiş) was lower, however, than that previously reported in cattle with reproductive problems from dairy farms in central and northern regions of the country (55.9%; Gavrea and Cozma, 2010). Compared to several other European countries, our seroprevalence was higher than that reported in Slovakia (20.1%; Reiterová et al, 2009) and Hungary (2.5%; Hornok et al, 2006), similar to that found in the northwest of Spain (23.2%; Eiras et al, 2011) but lower than that reported in Italy (32.0%; Rinaldi et al, 2007) and Turkey (46.5%; Kul et al, 2009).…”

supporting

confidence: 75%

“…Moreover, only a proportion of infected cows abort (Williams et al, 2000). In contrast, the results of other investigations carried out in Brazil (Corbellini et al, 2002), Japan (Koiwai et al, 2005), and Slovakia (Reiterová et al 2009) showed a higher seropositivity of antibodies to N. caninum in aborting cows then in non-aborting animals. Also, the finding in our study of no significant association between the seroprevalence and farming system is in contrast with findings of Wang et al (2010), who observed a significantly higher seroprevalence in grazing dairy cattle than that in cattle using confined feeding (28.6% vs. 10.7%).…”

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confidence: 71%

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Serological Survey of Neospora caninum Infection in Cattle Herds From Western Romania

Imre¹,

Morariu²,

Ilie³

et al. 2012

Journal of Parasitology

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Serum samples from 376 randomly selected adult cattle, from 25 farms located in 3 counties (Arad, Bihor, and Timiş) from western Romania, were sampled for Neospora caninum antibodies using a commercial ELISA-kit. Seroprevalence values and risk factors for neosporosis (cow age, breed, herd size, farming system, previous abortion, and number of farm dogs) were examined using a generalized linear mixed model with a binomial distribution. Overall, the seroprevalence of N. caninum was 27.7% (104/376) with a prevalence of 27.9% (24/86) in Arad, 26.9% (25/93) in Bihor, and 27.9% (55/197) in Timiş. Of 25 cattle herds, 23 were seropositive with a prevalence ranging from 10.0 to 52.2%. No correlation was found between N. caninum seropositivity and age, breed, herd size, breeding system, and previous abortion. The number of farm dogs was the only factor (P Wald 5 0.03) positively associated with seroprevalence in cows and can be considered the risk factor in the acquiring of infection. The present work is the first regarding serological evidence of N. caninum infection in cattle from western Romania.

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confidence: 75%

mentioning

confidence: 71%

Serological Survey of Neospora caninum Infection in Cattle Herds From Western Romania

Imre¹,

Morariu²,

Ilie³

et al. 2012

Journal of Parasitology

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“…In Slovakia, the presence of anti-Neospora antibodies in cattle and sheep post abortion was confirmed by Reiterová et al (2009) and Špilovská et al (2009). The literature offers several epidemiological studies on the incidence of antibodies against N. caninum in goats detected by different serological methods.…”

Section: Discussionmentioning

confidence: 81%

Toxoplasma gondii, Neospora caninum and tick-transmitted bacterium Anaplasma phagocytophilum infections in one selected goat farm in Slovakia

Čobádiová

Reiterová

Derdáková³

et al. 2013

Acta Parasitologica

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Parasitic diseases of livestock together with poor welfare conditions can negatively affect the health status and production of small ruminants. Protozoan parasites and tick-borne infectious agents are common threat of livestock including small ruminants mostly during the pasture season. Therefore the priority of the study was to analyse the circulation and presence of two protozoan parasites Toxoplasma gondii and Neospora caninum as well as tick-transmitted bacterium Anaplasma phagocytophilum in one selected goat farm in Eastern Slovakia. Throughout a three-year study period we have repeatedly screened the sera and blood of goats and dogs from monitored farm. In total, 343 blood serum samples from 116 goats were examined by ELISA. The mean seropositivity for T. gondii was 56.9% (66/116, CI (95%) = 48-66.0) and 15.5% (18/116, CI (95%) = 9.3-22.7) for N. caninum. The permanent occurrence of anti-Toxoplasma and anti-Neospora antibodies was detected in repeatedly examined goats during the whole monitored period. The presence of both parasites in the flock was analysed by PCR. DNA of T. gondii was confirmed in 12 out of 25 Toxoplasma-seropositive goats and N. caninum in 14 samples out of 18 Neospora-seropositive animals; four goats were co-infected with both pathogens. The risk of endogenous transmission of both parasites was pursued by examination of 41 kid's sera, where seropositivity for toxoplasmosis was 31.7% and for neosporosis 14.6%. In dogs 61.1% seropositivity for T. gondii and 38.9% for N. caninum was found, however, their faeces were negative for coccidian oocysts. Eight out of 108 tested animals were infected with A. phagocytophilum, the causative agent of tick-borne fever. Seven of them were simultaneously infected with T. gondii and A. phagocytophilum, out of which four goats were concurrently infected with all three pathogens.

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Section: Serological Analysismentioning

confidence: 99%

“…The adult livestock usually do not manifest clinical signs; abortions are often the only sign of infection (Dubey and Lindsay 1996, Hemphill 1999, Dubey 2003. The first serological study carried out by Reiterová et al (2009) in Slovakia revealed a high prevalence of N. caninum, mainly in farms located in north-eastern part of the country. The mean seroprevalence in Slovak spotted dairy cattle post-abortion was 20% and in cows without any reproduction problems only 2% have been recorded.…”

Section: Introductionmentioning

confidence: 99%

First in vitro isolation of Neospora caninum from a naturally infected adult dairy cow in Slovakia

Reiterová

Špilovská

Čobádiová

et al. 2011

Acta Parasitologica

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The impact of in vitro isolation and molecular characterisation of Neospora caninum as well as sequence analyses was studied. The brain homogenate of a naturally Neospora-infected dairy cow (positive in ELISA and Western blot) was intraperitoneally inoculated into Mongolian gerbils (Meriones unguiculatus). The brain of gerbils on day 60 post-inoculation was homogenized, and, after trypsin-digestion, cultured on Vero cells. Neospora-like tachyzoites were first observed after 77 days of cultivation. The parasite was confirmed by polymerase chain reaction (PCR) using Neospora-specific primers Np21 and Np6. The PCR product of the first Slovak isolate (NC-SKB1) was subsequently sequenced and published in GenBank under accession number GU300774. Sequencing and BLAST search identified the isolate as N. caninum.

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Neospora caninum, potential cause of abortions in dairy cows: The current serological follow-up in Slovakia

Cited by 25 publications

References 34 publications

Serological Survey of Neospora caninum Infection in Cattle Herds From Western Romania

Serological Survey of Neospora caninum Infection in Cattle Herds From Western Romania

Toxoplasma gondii, Neospora caninum and tick-transmitted bacterium Anaplasma phagocytophilum infections in one selected goat farm in Slovakia

First in vitro isolation of Neospora caninum from a naturally infected adult dairy cow in Slovakia

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