Neue enzymatische Methode zur Bestimmung von Nitrat in Lebensmitteln

Beutler, H. O.; Wurst, B.; Fischer, Sophie

doi:10.1007/bf00487944

Cited by 5 publications

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“…coli BWZ3 cells, respectively. Concentrations of nitrite were measured by a photometric diazo-dye assay from Boehringer after 1 h and 1 day of incubation at 30 °C with shaking at 230 rpm in a Falcon tube.…”

Section: Resultsmentioning

confidence: 99%

“…For in situ coupling to a consuming biocatalytic system, ATP was regenerated from ADP and AMP by kinases degrading polyphosphate ,− and can be tested by biocatalytic synthesis of flaviolin (Scheme A). NAD(P)H supply was achieved using phosphonate dehydrogenase ,,− supporting reductions: i.e., reducing redox indicators or enzymatic reduction of nitrate (Scheme B). SAM as a methyl donor for methyl transferases is provided by the ligation of ATP and methionine (Scheme C). ,, In the present work we adapted adequate cofactor supply systems for the application in permeabilized cells for the synthesis of secondary metabolites.…”

Section: Introductionmentioning

confidence: 99%

“…Regeneration of ATP was observed by a flaviolin synthesis assay, as previously described. 5 NADPH-dependent reduction of nitrate via nitrate reductase from Boehringer and subsequent quantification of diazo-dye formation was performed using the standard Boehringer NO 3 − / NO 2 − protocol, 31 on which phosphonate did not show any effect. Colorimetric detection was performed using a heλios α photometer.…”

Section: ■ Introductionmentioning

confidence: 99%

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Directed Multistep Biocatalysis for the Synthesis of the Polyketide Oxytetracycline in Permeabilized Cells of Escherichia coli

2015

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We report on tailored regeneration modules for the essential cofactors ATP and NADPH as well as for SAM supply, adapted to support biocatalytic multistep synthesis of oxytetracycline (OTC) in permeabilized cells of E. coli. ATP is regenerated from ADP and AMP by the use of polyphosphate. The efficiency of this system was proven with permeabilized cells producing flaviolin. NADPH is regenerated from NADP + by oxidation of phosphonate. A constant level of 0.7 mg/L of SAM is provided by the ligation of ATP and methionine. OTC is synthesized at a rate of 2.05 ng/(h (g of the combined biocatalyst)), reaching a final concentration of 0.9 mg/L.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

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Directed Multistep Biocatalysis for the Synthesis of the Polyketide Oxytetracycline in Permeabilized Cells of Escherichia coli

2015

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“…Subsequently, each sample was centrifuged and d ‐glucose and TRS concentrations were determined. Xylitol , ethanol , and acetic acid concentrations were also analyzed. All the analyses were carried out at least in duplicate.…”

Section: Methodsmentioning

confidence: 99%

Production of bioalcohols and antioxidant compounds by acid hydrolysis of lignocellulosic wastes and fermentation of hydrolysates with Hansenula polymorpha

Martínez-Cartas

Olivares

Sánchez

2019

Engineering in Life Sciences

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The effect of the H2SO4 concentration in the hydrolysis of sunflower‐stalk waste, at 95ºC and using a liquid/solid relation of 20, was studied. In a later stage, the hydrolysates were fermented at different temperatures with the aim of ethanol and xylitol production. A total conversion of the hemicellulose at the acid concentration of 0.5 mol/L was achieved; whereas an acid concentration of 2.5 mol/L was needed to reach the maximum value in the conversion of the cellulose fraction. The analysis of the hydrolysis kinetics has enabled to determine the apparent reaction order, which was 1.3. The hydrolysates from hydrolysis process with H2SO4 0.5 mol/L, once detoxified, were fermented at pH 5.5, temperatures 30, 40, and 50ºC with the yeast Hansenula polymorpha (ATCC 34438), resulting in a sequential uptake of sugars. In relation to ethanol and xylitol yields, the best results were observed at 50°C (YE/sO = 0.11 g/g; YXy/sO = 0.12 g/g). Instantaneous xylitol yields were higher than in ethanol, at the three temperatures essayed. Different phenolic compounds were analyzed in the hydrolysates; hydroxytyrosol was the most abundant (3.79 mg/L). The recovery of these compounds entails the elimination of inhibitors in the fermentation process and the production of high value‐added antioxidant products.

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Optical sensing of anions via polarity-sensitive dyes: A bulk sensor membrane for nitrate

Mohr

Wolfbeis

1995

Analytica Chimica Acta

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Neue enzymatische Methode zur Bestimmung von Nitrat in Lebensmitteln

Cited by 5 publications

References 0 publications

Directed Multistep Biocatalysis for the Synthesis of the Polyketide Oxytetracycline in Permeabilized Cells of Escherichia coli

Directed Multistep Biocatalysis for the Synthesis of the Polyketide Oxytetracycline in Permeabilized Cells of Escherichia coli

Production of bioalcohols and antioxidant compounds by acid hydrolysis of lignocellulosic wastes and fermentation of hydrolysates with Hansenula polymorpha

Optical sensing of anions via polarity-sensitive dyes: A bulk sensor membrane for nitrate

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