Neurite outgrowth promoting effects of enriched and mixed OEC/ONF cultures

Deumens, Ronald; Koopmans, Guido C; Lemmens, Marijke A.M.; Möllers, Sven; Honig, Wiel; Steinbusch, Harry W.M.; Brook, Gary; Joosten, Elbert A.J.

doi:10.1016/j.neulet.2005.11.063

Cited by 31 publications

(26 citation statements)

References 16 publications

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“…The use of GFP-expressing rat OECs for transplantation into a dorsal spinal cord transection (Sasaki et al, 2004) or demyelination (Sasaki et al, 2006b) lesion allowed clear identification of remyelination by engrafted OECs. More recently OECs have been considered to enhance repair of peripheral nerve fibers by possibly providing a scaffold for the regenerating axons as well as trophic factors and directional cues (Deumens et al, 2006). Indeed, transplantation of OECs into axotomized facial nerve was shown to enhance axonal sprouting (Guntinas-Lichius et al, 2001;Deumens et al, 2006), promote recovery of vibrissae motor performance (Guntinas-Lichius et al, 2002) and to increase the rate of eye closure (Choi and Raisman, 2005).…”

Section: Discussionmentioning

confidence: 99%

“…The rationale is that they may provide a scaffold for the regenerating axons as well as trophic factors and directional cues (Deumens et al, 2006). Transplantation of OECs into axotomized facial nerve has been shown to enhance axonal sprouting (Guntinas-Lichius et al, 2001;Deumens et al, 2006) and to promote the recovery of vibrissae motor performance (Guntinas-Lichius et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

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Myelination and nodal formation of regenerated peripheral nerve fibers following transplantation of acutely prepared olfactory ensheathing cells

et al. 2006

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Transplantation of olfactory ensheathing cells (OECs) into injured spinal cord results in improved functional outcome. Mechanisms suggested to account for this functional improvement include axonal regeneration, remyelination and neuroprotection. OECs transplanted into transected peripheral nerve have been shown to modify peripheral axonal regeneration and functional outcome. However, little is known of the detailed integration of OECs at the transplantation site in peripheral nerve. To address this issue cells populations enriched in OECs were isolated from the olfactory bulbs of adult green fluorescent protein (GFP)-expressing transgenic rats and transplanted into a sciatic nerve crush lesion which transects all axons. Five weeks to six months after transplantation the nerves were studied histologically. GFP-expressing OECs survived in the lesion and distributed longitudinally across the lesion zone. The internodal regions of individual teased fibers distal to the transection site were characterized by GFP expression in the cytoplasmic and nuclear compartments of cells surrounding the axons. Immuno-electron microscopy for GFP indicated that the transplanted OECs formed peripheral type myelin. Immunostaining for sodium channel and Caspr revealed a high density of Na v 1.6 at the newly formed nodes of Ranvier which were flanked by paranodal Caspr staining. These results indicate that transplanted OECs extensively integrate into transected peripheral nerve and form myelin on regenerated peripheral nerve fibers, and that nodes of Ranvier of these axons display proper sodium channel organization.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Myelination and nodal formation of regenerated peripheral nerve fibers following transplantation of acutely prepared olfactory ensheathing cells

et al. 2006

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“…Subsequent to the remyelination, OECs also replaced the myelin to regenerate disconnected axons and finally restored the neural conductance of the corticospinal tract (45,46). Furthermore, OEC transplantation also stimulated neurite regrowth to recover body movement in spinal cord injury (47,48).…”

Section: Figurementioning

confidence: 99%

“…In recent reports, OECs have been proven to promote neurite regrowth in vitro (48), and secreted neurotrophic factors and cell-cell contact mechanisms are probably involved in the neurite regeneration (48). Although previous reports have demonstrated that growth factors secreted from OECs such as nerve growth factor (NGF), BDNF, GDNF, and neurturin (NTN) might indirectly facilitate axon regeneration (52), the actual molecules involved in the cell-cell contact mechanism that directly mediated the neurite outgrowth-stimulating effect of hOECs/ONFs are not completely known.…”

Section: Figurementioning

confidence: 99%

Implantation of olfactory ensheathing cells promotes neuroplasticity in murine models of stroke

et al. 2008

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Murine olfactory ensheathing cells (OECs) promote central nervous system axonal regeneration in models of spinal cord injury. We investigated whether OECs could induce a neuroplastic effect to improve the neurological dysfunction caused by hypoxic/ischemic stress. In this study, human OECs/olfactory nerve fibroblasts (hOECs/ONFs) specifically secreted trophic factors including stromal cell-derived factor-1α (SDF-1α). Rats with intracerebral hOEC/ONF implantation showed more improvement on behavioral measures of neurological deficit following stroke than control rats.

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“…Generally, Schwann cells play an important role in peripheral nerve regeneration and remyelination by providing neurotrophic support, guiding axonal regeneration, and myelinating the regenerated axons. [1][2][3][4] As the patients with peripheral nerve injury do not receive timely treatment and do not recover completely, their quality of life will be affected to some extent. Some preclinical studies report that transplantation of olfactory ensheathing cells (OECs) for peripheral nerve injury in acute phase enhanced peripheral nerve restoration.…”

Section: Introductionmentioning

confidence: 99%

Olfactory ensheathing cell transplantation for a patient with chronic sciatic nerve injury

Zhang¹,

Meng²,

Lu³

et al. 2016

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Objective: To observe the result of olfactory ensheathing cell (OEC) transplantation in a patient with chronic sciatic nerve injury. Case report: A 53-year-old male patient with chronic (1 year) sciatic nerve injury on left side received OEC transplantation at the lesion site. He received follow-up assessment according to the American Spinal Injury Association standard at 10 days, 6 months, and 1 year after OEC therapy. The muscle strength of his left lower limb increased and numbness decreased during the early stage of cell therapy. His motor function improved with each evaluation. His limp walking gait recovered, and numbness sensation got nearly normal after 1 year of follow-up. There were no side effects. Conclusion: OEC transplantation may be an option for chronic peripheral (sciatic) nerve injury.

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Neurite outgrowth promoting effects of enriched and mixed OEC/ONF cultures

Cited by 31 publications

References 16 publications

Myelination and nodal formation of regenerated peripheral nerve fibers following transplantation of acutely prepared olfactory ensheathing cells

Myelination and nodal formation of regenerated peripheral nerve fibers following transplantation of acutely prepared olfactory ensheathing cells

Implantation of olfactory ensheathing cells promotes neuroplasticity in murine models of stroke

Olfactory ensheathing cell transplantation for a patient with chronic sciatic nerve injury

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