Neuronal beacon

Black, Bryan; Mondal, Angana; Kim, Y.; Mohanty, Samarendra

doi:10.1364/ol.38.002174

Cited by 12 publications

(17 citation statements)

References 17 publications

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“…For our experiments, we utilized a 1,455-nm infrared laser ( Fig. 1a ), which created a continuous heat source that was substantially more powerful than those used in previous optical guidance experiments 2 3 4 5 6 7 8 9 10 ( Fig. 1b ).…”

Section: Resultsmentioning

confidence: 99%

“…While chemical cues are the most intensively studied guidance factors 1 , physical guidance has recently been proposed. Of these, optical guidance is the representative non-invasive method for inducing attraction or retraction of growing neurites 2 3 4 5 6 7 8 9 10 . Various studies have utilized laser beams of different wavelengths to induce effective and accurate regulation of neurite outgrowth; however, the critical parameters and biological mechanism governing optical guidance remain elusive.…”

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confidence: 99%

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Triggering of high-speed neurite outgrowth using an optical microheater

Oyama

Zeeb

Kawamura

et al. 2015

Sci Rep

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Optical microheating is a powerful non-invasive method for manipulating biological functions such as gene expression, muscle contraction, and cell excitation. Here, we demonstrate its potential usage for regulating neurite outgrowth. We found that optical microheating with a water-absorbable 1,455-nm laser beam triggers directional and explosive neurite outgrowth and branching in rat hippocampal neurons. The focused laser beam under a microscope rapidly increases the local temperature from 36 °C to 41 °C (stabilized within 2 s), resulting in the elongation of neurites by more than 10 μm within 1 min. This high-speed, persistent elongation of neurites was suppressed by inhibitors of both microtubule and actin polymerization, indicating that the thermosensitive dynamics of these cytoskeletons play crucial roles in this heat-induced neurite outgrowth. Furthermore, we showed that microheating induced the regrowth of injured neurites and the interconnection of neurites. These results demonstrate the efficacy of optical microheating methods for the construction of arbitrary neural networks.

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Triggering of high-speed neurite outgrowth using an optical microheater

Oyama

Zeeb

Kawamura

et al. 2015

Sci Rep

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“…Though light has been shown to act as an attractive cue while directly impinging on the growth cone (with specific irradiation schedules), we have utilized an at-a-distance effect using a weakly-focused NIR light source which we currently believe to be photothermal2829. For loop-formation reported here, axons advancing at a growth rate of > 20 μm/hr were randomly selected.…”

Section: Resultsmentioning

confidence: 99%

“…While existing optical methods are based on attractive-guidance principles, we have found that a near-infrared (NIR) laser beams can act as a repulsive cue2728. Here, we have utilized a weakly-focused NIR laser beam to guide rat cortical axons in a highly effective manner, which has allowed us to construct loops of varying radii and observe axonal fasciculation for the first time.…”

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confidence: 99%

Loop formation and self-fasciculation of cortical axon using photonic guidance at long working distance

Mondal

Black²,

Kim

et al. 2014

Sci Rep

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The accuracy of axonal pathfinding and the formation of functional neural circuitry are crucial for an organism to process, store, and retrieve information from internal networks as well as from the environment. Aberrations in axonal migration is believed to lead to loop formation and self-fasciculation, which can lead to highly dysfunctional neural circuitry and therefore self-avoidance of axons is proposed to be the regulatory mechanism for control of synaptogenesis. Here, we report the application of a newly developed non-contact optical method using a weakly-focused, near infrared laser beam for highly efficient axonal guidance, and demonstrate the formation of axonal loops in cortical neurons, which demonstrate that cortical neurons can self-fasciculate in contrast to self-avoidance. The ability of light for axonal nano-loop formation opens up new avenues for the construction of complex neural circuitry, and non-invasive guidance of neurons at long working distances for restoration of impaired neural connections and functions.

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“…As the axon senses the repulsive cue, it deviates from its initial path to avoid the light (Fig. 3.14 ) (Black et al 2013 ). It is believed that this is a photothermal phenomenon in which fi lopodia sense and respond to a laser induced thermal gradient.…”

Section: Neuronal Beaconmentioning

confidence: 99%

Neural Engineering

2016

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Neuronal beacon

Cited by 12 publications

References 17 publications

Triggering of high-speed neurite outgrowth using an optical microheater

Triggering of high-speed neurite outgrowth using an optical microheater

Loop formation and self-fasciculation of cortical axon using photonic guidance at long working distance

Neural Engineering

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