Neuroprotective Activity of Lavender Oil on Transient Focal Cerebral Ischemia in Mice

Abstract

The air-dried aerial parts of Lavandula angustifolia Mill, a traditional Uygur herbal drug, is used as resuscitation-inducing therapy to treat neurodisfunctions, such as stroke. This study was designed to assess the neuroprotective effects of lavender oil against ischemia/reperfusion (IR) injury in mice. Focal cerebral ischemia was induced by the intraluminal occlusion method with a nylon string. The neurodysfuntion was evaluated by neurological deficit and the infarct area was showed by 2,3,5-triphenyltetrazolium chloride (TTC) staining. The histopathological changes were observed by hematoxylin and eosin staining. The levels of mitochondria-generated reactive oxygen species (ROS), malondialdehyde (MDA) and carbonyl, the ratio of reduced glutathione (GSH)/glutathione disulfide (GSSG), the activities of superoxide dismutase (SOD), catalase (CAT) and glutathion peroxidase (GSH-Px) in brain tissue were measured to estimate the oxidative stress state. Neurological deficit, infarct size, histopathology changes and oxidative stress markers were evaluated after 22 h of reperfusion. In comparison with the model group, treatment with lavender oil significantly decreased neurological deficit scores, infarct size, the levels of MDA, carbonyl and ROS, and attenuated neuronal damage, upregulated SOD, CAT, GSH-Px activities and GSH/GSSG ratio. These results suggested that the neuroprotective effects of lavender oil against cerebral ischemia/reperfusion injury may be attributed to its antioxidant effects.

Role of mitochondrial function in the protective effects of ischaemic postconditioning on ischaemia/reperfusion cerebral damage

“…Others have shown that chemicals with protective effects on cerebral injury reverse damage to mitochondrial membrane potential. 5,6 It is possible that the protective effect of ischaemic postconditioning is also related to improvements in mitochondrial membrane potential.…”

“…2,3 Antioxidants have been shown to have therapeutic effects on ischaemia/reperfusion brain injury, suggesting that oxidative stress has an active role in modulating neuronal destiny. [4][5][6] In addition, other neurological disorders (including neurodegenerative diseases and traumatic brain injury) are related to oxidative stress. 7,8 The crucial feature of oxidative stress is overproduction of reactive oxygen species (ROS) resulting from disruption to the equilibrium between ROS formation and clearance.…”

Involvement of AMP-activated protein kinase in the protective effectof melatonin against renal ischemia reperfusion injury

“…Restitution of blood flow with reperfusion sustains renal injury through further generation of ROS (Bidmon et al, 2012). ROS are capable of reacting with proteins, lipids, and nucleic acids leading to protein denaturation, lipid peroxidation, and DNA damage (Bidmon et al, 2012;Wang et al, 2012a). Many antioxidants have been the focus of studies for developing strategies for use in I/R therapy (Mahfoudh Boussaid et al, 2007;Wang et al, 2012a;Aydogan et al, 2013).…”

“…ROS are capable of reacting with proteins, lipids, and nucleic acids leading to protein denaturation, lipid peroxidation, and DNA damage (Bidmon et al, 2012;Wang et al, 2012a). Many antioxidants have been the focus of studies for developing strategies for use in I/R therapy (Mahfoudh Boussaid et al, 2007;Wang et al, 2012a;Aydogan et al, 2013). Along with others, we have shown that the antioxidant molecule, melatonin, has a significant protective action against renal damage that occurs during I/R injury (Aktoz et al, 2007;Ersoz et al, 2009;Hadj Ayed Tka et al, 2015).…”

Biological effects of four frequently used medicinal plants of Lamiaceae

“…This suggests that the regular use of lavander oil in skin preparations could suppress the aging effects of sunlight on the skin. Wang et al [47] found that treatment with lavander oil significantly decreased neurological deficit scores, infarct size, the levels of malondialdehyde, carbonyl and reactive oxygen species (ROS), and attenuated neuronal damage, upregulated antioxidant enzymes such as superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) activities. These results suggested that the neuroprotective effects of lavander oil against cerebral ischemia/reperfusion injury in mice may be attributed to its antioxidant effects [47].…”