Neuroprotective Effects of Pomegranate Juice on Lead Induced Neurotoxicity in Mice

Abstract

<p><strong>Objective: </strong>This study evaluates the potential neuroprotective of the pomegranate juice against chronic intoxication with lead acetate for 3<strong> </strong>months.</p><p><strong>Methods: </strong>Twenty-one female Swiss mice divided into 3 groups were employed in the present investigation. Control group: received drinking water for 90 days, neurotoxic group were exposed to 1000 ppm of lead acetate in the drinking water for 12 weeks, and neurotoxic treated group represents the mice received treatment with juice pomegranate diluted with distilled water (v/v) orally for 4 h / day followed by lead acetate at a dose of 1000 ppm orally for 20 h / day for 90 days. After cessation of treatment, neurobehavioral studies using the open field test, black and white test box and swimming test were made. In the next phase, brain injury was assessed histologically with hematoxylin-eosin staining.</p><p><strong>Results:</strong> Chronic exposure to lead led to significant increase in the level of anxiety, depression and the locomotor activity (P < 0.05). It was confirmed by histopathological alterations in many areas of the cerebral cortex and hippocampus including neuronal degeneration and decrease cell density. Treatment with the juice significantly improve the level of depression, locomotor function (P < 005) and anxiety (P > 0.05) in mice exposed to lead as well as restored the histological structure in cerebral cortex and hippocampus of mice. The total phenolic and flavonoids content in juice of pomegranate was found to be 3809. 8±29.404 mg GAE/l; 2109. 57±18.936 mg QE /l of juice.</p><p><strong>Conclusion: </strong>This finding suggests that phenolic compounds found in pomegranate juice provide a neuroprotective effect on behavioural impairments and histopathological change induced by lead.</p>

The protective effects of pomegranate juice on lead acetate‐induced neurotoxicity in the male rat: A histomorphometric and biochemical study

“…Exposure to lead may therefore have led to volume loss in the brain by producing adverse effects on cell signalling, neurotransmission and cell redox state. Examination of the scientific literature shows that there have been very few studies of the effects of PJ on LA‐induced neurotoxicity (Aksu et al., 2016; Gadouche et al., 2017). Aksu et al showed that PJ may provide significant benefits against exposure to lead.…”

Detrimental Effects of Lead on Human Health and Protective Effect by Natural Polyphenols: A Review

“…Rich polyphenolic components present in pomegranate juice caused beneficial effect on behavioural impairments and histopathological changes against chronic intoxication with lead acetate for 3 months. 69 Polyphenols present in methanolic extract of Indigofera oblongifolia leaf significantly protected the neuronal damage which was induced by five days intra gastric administration of lead acetate through oral route. 70 Treatment with tertiary butylhydroquinone which a phenolic organic compound is reflected decrease in MDA level and increase in SOD and GSH content, in the hippocampus and frontal cortex of rat.…”

The Potency of Indonesia’s Pomegranate Peel Ethanol Extract (Punica Granatum Linn.) as Anti-Inflammatory Agent in Mice Colon Induced by Dextran Sodium Sulfate: Focus on Cyclooxygenase-2 and Inos Expressions

“…Currently, some medicinal herbs are widely studied as UC therapies such as apples, blackberries, green tea, curcuma, and pomegranate [15]. Some of the medicinal herb mentioned, pomegranate is one of the plants that have been studied many effects such as anti-inflammatory, anticancer, antibacterial, antioxidants, and neuroprotective [16][17][18][19]. Pomegranate peel possesses higher content of polyphenols.…”