Neurosphere generation from dental pulp of adult rat incisor

Abstract

Dental pulp is a potential source of cells that can be used in cell replacement therapy for various nervous system disorders. Here we report that adult rat dental pulp cells have the ability to form neurospheres when cultured in serum-free culture medium on super-hydrophilic plates. The cells within small spheres continued to grow, and the dental pulp-derived cells generated large spheres. Sphere formation was dependent on exogenously supplied basic-fibroblast growth factor, but not on epidermal growth factor, and the formation and growth of dental pulp-derived spheres were negatively regulated by transforming growth factor-beta. Plating cells that were dissociated from spheres on an adhesive substrate resulted in differentiation into Tuj1- and MAP2-positive neuronal cells. Analysis of the three-dimensional structure of dental pulp-derived spheres shows that they contained nestin-positive progenitors, Tuj1-positive neuronal cells and S100-positive glial cells. We found that spheres contained CD81 (TAPA1) and nestin double-positive cells, and identified a small population of CD81 and nestin double-positive cells in the odontoblast layer of the dental pulp. Flow cytometric analysis showed that CD81-positive cells were enriched in the spheres compared with the dental pulp tissue. Bromodeoxyuridine (BrdU) staining showed that nestin- and BrdU-positive cells were located only in the apical portion of the dental pulp, and the apical portion produced a large number of large-sized spheres. These data suggest that the CD81 and nestin double-positive cells localized in the odontoblast layer of the apical portion of the dental pulp may have the ability to grow and form neurospheres.

Derivation and growth characteristics of dental pulp stem cells from patients of different ages

“…In the present study, the differentiated cells from DPSCs were positive for specific markers for neurons and gliocytes. In rats, DPSCs were able to differentiate into nestin-positive progenitors, TUJ-1-positive neuronal cells and S100-positive glial cells (38). Furthermore, human postnatal dental pulp cells were demonstrated to be able to differentiate into class III beta tubulin and TUJ-1 positive neuronal cells (39).…”

A Journey from Dental Pulp Stem Cells to a Bio-tooth

“…Thus, it is reasonable to assume that DPSCs are derived from CNC ectomesenchyme [40]. Sasaki et al have demonstrated that DPSCs contain primitive stem cell subpopulations of neural crest origin, including Nestin + precursor cells, Tuj1 + neuron cells, and S100 + glial cells [41]. These stem cells can generate the neurospheres under the serum-free culture conditions.…”

Expression of Multiple Stem Cell Markers in Dental Pulp Cells Cultured in Serum-free Media

“…DTPC and WTPC suspensions were plated at a cell density of 1 Â 10 5 cells per 25-cm 2 flask. Four types of serum-free growth medium consisting of DMEM with antibiotic and antimycotic additives were used: SFM#1 supplemented with 1% insulin-transferrin-selenium-X (ITS-X) (Invitrogen) (3, 7-9) and 100 mg/mL of embryotrophic factor (ETF) (a gift from Prof. Hiroshi Ishikawa, Nippon Dental University) (10); SFM#2 supplemented with 1% ITS-X; SFM#3 supplemented with 100 mg/mL ETF; and SFM#4 supplemented with 100 mg/mL ETF, 1 mmol/ L sodium pyruvate (Invitrogen) (7,11,12), 25 mg/mL ascorbic acid (Wako Pure Chemical Industries Ltd) (12,13), and 4 ng/mL fibroblast growth factor, acidic (FGF-a) (Biomedical Technologies Inc, Stoughton, MA) (8,9,11,(14)(15)(16)(17)(18)(19). We used normal medium containing DMEM and 10% FBS as a positive control.…”