Neurotensin‐like immunoreactivity in the preganglionic sympathetic nerves and in the adrenal medulla of the cat

Lundberg, Jan M.; Rökaeus, Åke; Hökfelt, Tomas; Rosell, Sune; Brown, Marvin; Goldstein, Menek

doi:10.1111/j.1748-1716.1982.tb06965.x

Cited by 102 publications

(21 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Neurotensin-like immunoreactive material (NTLI) has been identified immunohistochemically in perikarya and fibres of neurones in the central nervous system (Carraway & Leeman, 1973, 1976aKobayashi, Brown & Vale, 1977;Uhl & Snyder, 1981), in nerve fibres of the intrinsic intramural plexus of the gastrointestinal tract (Schultzberg, Hokfelt, Nilsson, Terenius, Rehfeld, Brown, Elde, Goldstein & Said, 1980: Reinecke et al 1983: Leander, Ekman, Uddman, Sundler & Hakanson, 1984 and in preganglionic sympathetic nerve fibres and their terminal network around neurones of prevertebral ganglia in cat and guinea-pig (Lundberg et al 1980(Lundberg et al , 1982Reinecke et al 1983;Heym et al 1984).…”

Section: Discussionmentioning

confidence: 99%

The electrophysiological effects of neurotensin on neurones of guinea‐pig prevertebral sympathetic ganglia.

Stapelfeldt

Szurszewski

1989

The Journal of Physiology

View full text Add to dashboard Cite

SUMMARY1. The membrane effects of neurotensin on neurones of guinea-pig prevertebral ganglia were investigated by means of intracellular recording techniques in vitro.2. Neurotensin (2-5 ,UM) applied by superfusion caused depolarizing responses in fifty-seven of seventy-four neurones tested in the inferior mesenteric ganglion and thirty-seven of forty-seven neurones tested in the coeliac plexus. The remaining neurones tested showed no membrane response.3. Responses to neurotensin could be discriminated into two different types of membrane depolarizations on the basis of their different time courses and pharmacological characteristics: a steady-state type of depolarization and a transient type of depolarization. Seven of fifty-seven responsive neurones tested in the inferior mesenteric ganglion and ten of thirty-seven responsive neurones tested in the coeliac plexus responded to neurotensin with a depolarization which was maintained constant as long as neurotensin was superfused over the preparation (steady-state type). Forty-eight of fifty-seven responsive neurones tested in the inferior mesenteric ganglion and twenty of thirty-seven responsive neurones tested in the coeliac plexus responded with a transient depolarization which was followed by a repolarization in the maintained presence of neurotensin (transient type). A combination of both types of responses was observed in two neurones tested in the inferior mesenteric ganglion and in seven neurones tested in the coeliac plexus.4. Steady-state type responses were characterized by a slowly developing membrane depolarization which reached a plateau and lasted throughout the presence of neurotensin. Amplitude and time course of this response were not altered in a solution containing hexamethonium (10 ram) and atropine (10 tM) or by a solution low in calcium (1 mM) and high in magnesium (15 mM).5. Transient type depolarizations evoked by neurotensin were faster in reaching their maximum and were followed by a repolarization during the maintained presence of neurotensin. Responses similar in time course and amplitude were obtained in solutions containing hexamethonium (10-100 /tM) and atropine (10 /IM).However, transient responses were abolished in a solution low in calcium (1 mM) and high in magnesium (15 mM) and were markedly attenuated in ganglia treated with capsaicin (3 /tM). 7. Both types of depolarizations were observed when the C-terminal hexapeptide fragment neurotensin 8-13 was used.8. It was concluded that neurotensin caused an increase in excitability in a large portion of prevertebral sympathetic neurones of guinea-pigs by interaction with receptors specific for the C-terminal, bioactive portion of the molecule. The response of the large majority of neurones was mediated through Ca2+-dependent release of a non-cholinergic excitatory transmitter from capsaicin-sensitive nerve terminals.

show abstract

Section: Discussionmentioning

confidence: 99%

The electrophysiological effects of neurotensin on neurones of guinea‐pig prevertebral sympathetic ganglia.

Stapelfeldt

Szurszewski

1989

The Journal of Physiology

View full text Add to dashboard Cite

show abstract

“…Neurotensin-like immunoreactivity has been found by radioimmunoassay in the adrenal medulla of rats, guinea pigs, rabbits, cats and bovines, among which the highest level was seen in the cat (CoRDER et al, 1982;LUNDBERG et al, 1982;GOEDERT and EMSON, 1983;. In a chromaffin granule preparation from the bovine adrenal medulla, neurotensin-like immunoreactivity was concentrated in a manner identical to CA .…”

Section: Neurotensinmentioning

confidence: 99%

Immunochistochemical Analysis of the Localization of Neuropeptides in the Adrenal Gland

Kondo

1985

Arch. Histol. Jap., Arch. Histol. Jpn

127

View full text Add to dashboard Cite

“…Recent immunohistochemical studies, however, have shown that the cells synthesize, store and release a variety of bioactive peptides such as enkephalins (SCHULTZBERG et al, 1978;LINNOILA et al, 1980;PELTO-HUIKKO et al, 1982;LIVETT et al, 1982;KOBAYASHI et al, 1983a), substance P ( KURAMOTO et al, 1985b), neuropeptide Y (NPY) (VARNDELL et al, 1984;KURAMOTO et al, 1986;LUNDBERG et al, 1986;review;SUNDLER et al, 1986), vasoactive intestinal polypeptide (VIP) (BRYANT et al, 1976;HOLZWARTH 1984;KONDO et al, 1986), somatostatin (LUNDBERG et al, 1979), neurotensin (LUNDBERG et al, 1982a) and calcitonin gene-related peptide (CGRP) (RosENFELD et al, 1983 ;KURAMOTO et al, 1987). In addition, most of these peptides have been revealed to be contained not only in the chromaffin cells, but also in the nerve fibers supplying them.…”

mentioning

confidence: 99%

An immunohistochemical study of chromaffin cells and nerve fibers in the adrenal gland of the bullfrog, Rana catesbeiana.

Kuramoto

1987

Arch. Histol. Jap., Arch. Histol. Jpn

View full text Add to dashboard Cite

Neurotensin‐like immunoreactivity in the preganglionic sympathetic nerves and in the adrenal medulla of the cat

Cited by 102 publications

References 5 publications

The electrophysiological effects of neurotensin on neurones of guinea‐pig prevertebral sympathetic ganglia.

The electrophysiological effects of neurotensin on neurones of guinea‐pig prevertebral sympathetic ganglia.

Immunochistochemical Analysis of the Localization of Neuropeptides in the Adrenal Gland

An immunohistochemical study of chromaffin cells and nerve fibers in the adrenal gland of the bullfrog, Rana catesbeiana.

Contact Info

Product

Resources

About