Neutrophil elastase is involved in the initial destruction of human periodontal ligament

Ujiie, Yuko; Oida, Shinichiro; Gomi, Kazuhiro; Arai, Takashi; Fukae, Makoto

doi:10.1111/j.1600-0765.2006.00952.x

Cited by 31 publications

(34 citation statements)

References 18 publications

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“…Polymorphonuclear leukocyte elastase was observed in the periapical tissue of patients clinically diagnosed as having irreversible pulpitis (24). Neutrophil elastase is detected in the periodontal ligament of patients with periodontitis (33). Although the neutrophil elastase was already known to be increased in the inflammation of other tissues, the increase was investigated in the periapical tissue with inflammation.…”

Section: Discussionmentioning

confidence: 98%

The Expression of Macrophage and Neutrophil Elastases in Rat Periradicular Lesions

Morimoto

Yamasaki

Nakata

et al. 2008

Journal of Endodontics

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Section: Discussionmentioning

confidence: 98%

The Expression of Macrophage and Neutrophil Elastases in Rat Periradicular Lesions

Morimoto

Yamasaki

Nakata

et al. 2008

Journal of Endodontics

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“…16,17 Neutrophil elastase is the most important enzyme that degrades fibronectin to Fn fragments in vivo . 18,19 The activity of neutrophils to release elastase is significantly increased in periodontitis patients than in healthy controls. 20,21,22 An increase in neutrophil elastase activity increases fibronectin degradation fragments and decreases periodontal mechanical strength.…”

Section: Discussionmentioning

confidence: 99%

Expression of cellular fibronectin mRNA in adult periodontitis and peri-implantitis: a real-time polymerase chain reaction study

Cao

Kang

et al. 2013

Int J Oral Sci

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Cellular fibronectin (cFn) is a type of bioactive non-collagen glycoprotein regarded as the main substance used to maintain periodontal attachment. The content of cFn in some specific sites can reflect the progress of periodontitis or peri-implantitis. This study aims to evaluate the expression of cFn messenger RNA (mRNA) in tissues of adult periodontitis and peri-implantitis by real-time fluorescent quantitative polymerase chain reaction (PCR) and to determine its clinical significance. A total of 30 patients were divided into three groups of 10: healthy, adult periodontitis and peri-implantitis. Periodontal tissue biopsies (1 mm×1 mm×1 mm) from each patient were frozen in liquid nitrogen. Total RNA was extracted from these tissues, and the content, purity and integrity were detected. Specific primers were designed according to the sequence, and the mRNA expression levels of cellular fibronectin were detected by real-time PCR. The purity and integrity of the extracted total RNA were both high, and the specificity of amplified genes was very high with no other pollution. The mRNA expression of cFn in the adult periodontitis group (1.526±0.441) was lower than that in the healthy group (3.253±0.736). However, the mRNA expression of cFn in the peri-implantitis group (3.965±0.537) was significantly higher than that in the healthy group. The difference revealed that although both processes were destructive inflammatory reactions in the periodontium, the pathomechanisms were different and the variation started from the transcription level of the cFn gene.

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“…[15] Experiments have also demonstrated that the neutrophil elastase is involved in the initial destruction of periodontal ligament during early stages of periodontal disease. [16] Reports have shown that the SLPI plays an important role in controlling salivary elastase activity. [17] These findings support the findings from the present study, viz., increased SLPI levels in group A and control group when compared to group B…”

Section: Discussionmentioning

confidence: 99%

Role of salivary leukocyte protease inhibitor in periodontal disease progression

Pateel

Seema

Kale

2010

J Indian Soc Periodontol

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Context:Proteases play a major role in the tissue destruction involved in periodontal disease. It is known that the balance between proteases and their inhibitors is a major determinant in maintaining tissue integrity. The association between the proteases and periodontitis is well established, but not many studies have been carried out to know the role played by a protease inhibitor like salivary leukocyte protease inhibitor (SLPI) in periodontitis.Aim:The aim of the present study was to correlate SLPI with periodontitis.Settings and Design:Case-control study.Materials and Methods:Seventy-five clinically confirmed cases of periodontitis and 20 controls were included in the study. A detailed case history and periodontal index (PI) were recorded. Two milliliters of unstimulated saliva samples was obtained and subjected to quantification of SLPI leaves using SLPI in enzyme-linked immunosorbent assay (ELISA) kit. Based on the periodontal index score of the individuals, the cases and controls were divided into groups A, B and C, and the obtained SLPI levels were compared among the groups.Statistical Analysis:Mann-Whitney U test and correlation coefficient test.Results:The results showed that in the initial stages of periodontitis there is a tendency of SLPI levels to be raised. The SLPI levels were found to be reduced in the terminal stages of periodontitis.Conclusion:It appears that SLPI accumulates in the local environment, at least in the initial stages of the periodontal disease, probably to inhibit the action of increased elastic activity.

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Neutrophil elastase is involved in the initial destruction of human periodontal ligament

Abstract: It was concluded that neutrophil elastase is involved in the degradation of noncollagenous protein-covered collagen fibrils in the early destructive stages of periodontal disease.

Cited by 31 publications

References 18 publications

The Expression of Macrophage and Neutrophil Elastases in Rat Periradicular Lesions

The Expression of Macrophage and Neutrophil Elastases in Rat Periradicular Lesions

Expression of cellular fibronectin mRNA in adult periodontitis and peri-implantitis: a real-time polymerase chain reaction study

Role of salivary leukocyte protease inhibitor in periodontal disease progression

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