New advances in electrochemical biosensors for the detection of toxins: Nanomaterials, magnetic beads and microfluidics systems. A review

Reverté, Laia; Prieto‐Simón, Beatriz; Campàs, Mònica

doi:10.1016/j.aca.2015.11.050

Cited by 171 publications

(89 citation statements)

References 88 publications

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“…In recent years combining nanotechnology with the various available bio sensing techniques is bringing to life the so-called “nano-biosensors” which demonstrate rapid responses combined with high sensitivities. [67,68•,69•]…”

Section: Introductionmentioning

confidence: 99%

Nanotechnology to the rescue: using nano-enabled approaches in microbiological food safety and quality

Eleftheriadou

Pyrgiotakis²,

Demokritou³

2017

Current Opinion in Biotechnology

139

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Food safety and quality assurance is entering a new era. Interventions along the food supply chain must become more efficient in safeguarding public health and the environment and must address numerous challenges and new consumption trends. Current methods of microbial control to assure the safety of food and minimize microbial spoilage have each shown inefficiencies. Nanotechnology is a rapidly expanding area in the agri/feed/food sector. Nano-enabled approaches such as antimicrobial food-contact surfaces/packaging, nano-enabled sensors for rapid pathogen/contaminant detection and nano-delivered biocidal methods, currently on the market or at a developmental stage, show great potential for the food industry. Concerns on potential risks to human health and the environment posed by use of engineered nanomaterials (ENMs) in food applications must, however, be adequately evaluated at the developmental stage to ensure consumer's acceptance.

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Section: Introductionmentioning

confidence: 99%

Nanotechnology to the rescue: using nano-enabled approaches in microbiological food safety and quality

Eleftheriadou

Pyrgiotakis²,

Demokritou³

2017

Current Opinion in Biotechnology

139

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“…In this context, piezoelectric biosensors using quartz chips are particularly attractive since they provide quantitative information on biomolecular interactions at the solid -liquid interface in real time and label-free fashion and allow the detection of high molecular weight targets at the ng level [26]. Recently, interest has been drawn towards the contribution of nanomaterials in the development of biosensors for toxin analysis [27,28]. Among nanomaterials, gold nanoparticles (AuNP) have attracted special interest in the field of biosensor development owing to their outstanding physico-chemical features and their ease of synthesis and functionalization [29][30][31].…”

Section: Introductionmentioning

confidence: 99%

Gold colloid-nanostructured surfaces for enhanced piezoelectric immunosensing of staphylococcal enterotoxin A

Haddada

Salmain

Boujday

2018

Sensors and Actuators B: Chemical

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Highlights Gold-and silicon-coated quartz sensors were nanostructured with gold nanoparticles  A capture layer comprising an anti-SEA antibody was built up on the sensor surface  Nanostructuration improved sensor response owing to better accessibility of antibodies  With a sandwich-type assay, the limit of detection was 1 ng SEA/mL in 25 min AbstractWe describe the use of gold nanoparticles (AuNP) as a nanostructuring agent on quartz crystal sensor chips to engineer staphylococcal enterotoxin A (SEA) piezoelectric biosensors with amplified response. AuNPs were assembled on gold-or silicon-coated quartz crystal sensor chips by a wet chemistry process involving their chemisorption to preformed thiol and amine terminated Self-Assembled Monolayers (SAMs). The purpose of this nanostructuration was to modify the topography of the surface and improve the accessibility of the binding sites on the surface of the sensor chips. Biointerfaces, comprising a polyclonal antibody against staphylococcal enterotoxin A (SEA), were further built up on these gold nanoparticle-coated sensors and their ability to capture SEA was monitored in real time with a quartz crystal microbalance with dissipation monitoring. It was found out that, although the surface density in capture antibody was similar on both nanostructured and planar sensors, the sensor response, expressed as frequency shift recorded during the binding of SEA to the antibody, was significantly higher for the nanostructured sensors as compared to the planar ones. All the same, the limit of detection was lower for the nanostructured sensors: 8 ng/mL vs 20 ng/mL for the planar sensors. This was rationalized by a possibly better accessibility of the antigen binding sites rather than a consequence of specific surface increase. Using a sandwich type assay, gold nanoparticles coated silicon quartz sensor chips provided the lowest limit of detection of ca. 1 ng/mL in a total assay time of 25 min. 2

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“…Antibodies can be placed in a microfluidic setup by device surface modification [11, 12] or through a solid support like porous polymer monoliths [13], beads [14, 15] or nanoparticles [16, 17] introduced into microchannels.…”

Section: On-chip Sample Preparation Methodsmentioning

confidence: 99%

“…Beads are routine solid supports for antibodies and can be easily manipulated within fluidic networks [14]; thus, microfluidic systems that utilize beads for immuno-capture and detection of disease biomarkers are being developed [15]. Gao et al [29] used antibody-conjugated magnetic beads for the detection of an anthrax biomarker, poly-γ-D-glutamic acid (PGA), in human serum using surface-enhanced Raman scattering (SERS) in a microfluidic setup, shown in Fig.…”

Section: On-chip Sample Preparation Methodsmentioning

confidence: 99%

Recent advances in microfluidic sample preparation and separation techniques for molecular biomarker analysis: A critical review

Sonker

Sahore

Woolley

2017

Analytica Chimica Acta

141

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Microfluidics is a vibrant and expanding field that has the potential for solving many analytical challenges. Microfluidics show promise to provide rapid, inexpensive, efficient, and portable diagnostic solutions that can be used in resource-limited settings. Researchers have recently reported various microfluidic platforms for biomarker analysis applications. Sample preparation processes like purification, preconcentration and labeling have been characterized on-chip. Additionally, improvements in microfluidic separation techniques have been reported for cellular and molecular biomarkers. This review critically evaluates microfluidic sample preparation platforms and separation methods for biomarker analysis reported in the last two years. Key advances in device operation and ability to process different sample matrices in a variety of device materials are highlighted. Finally, current needs and potential future directions for microfluidic device development to realize its full diagnostic potential are discussed.

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New advances in electrochemical biosensors for the detection of toxins: Nanomaterials, magnetic beads and microfluidics systems. A review

Cited by 171 publications

References 88 publications

Nanotechnology to the rescue: using nano-enabled approaches in microbiological food safety and quality

Nanotechnology to the rescue: using nano-enabled approaches in microbiological food safety and quality

Gold colloid-nanostructured surfaces for enhanced piezoelectric immunosensing of staphylococcal enterotoxin A

Recent advances in microfluidic sample preparation and separation techniques for molecular biomarker analysis: A critical review

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