New and bioactive natural products from an endophyte of Panax notoginseng

Xie, Jun; Wu, Ying‐Ying; Zhang, Tianyuan; Zhang, Mengyue; Zhu, Weiwei; Gullen, Elizabeth A.; Wang, Zhaojie; Cheng, Yung‐Chi

doi:10.1039/c7ra07060h

“…The plant has been cultivated and domesticated for approximately 400 years, mainly in Yunnan and Guangxi provinces, China. Continuous cultivation of P. notoginseng in the same field will led it to be attacked vulnerably by various soil-borne pathogens, like fungi, bacteria and nematodes [ 9 ]. The rhizospheric and endophytic fungal communities are considered not only of vital importance for plant health and soil fertility, but also to have positive effects on plant resistance to diseases and insects.…”

Section: Introductionmentioning

confidence: 99%

Two New Alkaloids from Fusarium tricinctum SYPF 7082, an Endophyte from the Root of Panax notoginseng

Sun

¹

,

Zhu

²

,

Zhang

³

et al. 2018

Nat. Prod. Bioprospect.

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Panax notoginseng (Araliaceae) is a famous traditional Chinese medicine mainly cultivated in Yunnan and Guangxi provinces of China. Two new alkaloids, rigidiusculamide E (1) and [-(α-oxyisohexanoyl-N-methyl-leucyl)2-] (2), together with two known ones, (−)-oxysporidinone (3) and (−)-4,6′-anhydrooxysporidinone (4) were isolated from the mycelia culture of Fusarium tricinctum SYPF 7082, an endophytic fungus obtained from the healthy root of P. notoginseng. Their structures were determined on the basis of extensive spectroscopic analyses. Compounds 1–4 were tested for their inhibitory effects against NO production on Murine macrophage cell line, and the new compound 2 showed significant inhibitory activity on NO production with the IC50 value of 18.10 ± 0.16 μM.Graphical Abstract Electronic supplementary materialThe online version of this article (10.1007/s13659-018-0171-0) contains supplementary material, which is available to authorized users.

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“…tenuiussimum , P. janthinellum ) and four pathogens (bacteria: A. marplatensis, N. rhamnosiphila ; fungi: Cl. gossypicola, F. oxysporium ) from the rhizosphere soil of P. notoginseng were tested according to the methods of previous studies ( Xie et al., 2017 , Liu et al., 2010 , Zhang et al., 2004 ). At a concentration of 0.1 mg mL −1 , compounds 2 and 5 showed substantial antibacterial activity on Ba.…”

Section: Resultsmentioning

confidence: 99%

“…Previous studies on rhizosphere microorganisms of P. notoginseng have mainly focused on the microbial community structure and the pathogenic effect of microorganisms on the plant ( Miao et al., 2015 , Guan et al., 2005 , Guan et al., 2006 , Guan et al., 2010 ). Only one study has reported the inhibitory effects of the secondary metabolites from endophytic fungi of P. notoginseng on other rhizosphere microorganisms ( Xie et al., 2017 ). In the present study, compounds 3 , 4 , 6 and 7 significantly inhibited the growth of probiotics and promoted the growth of pathogens, at a concentration of 0.1 mg mL −1 , indicating that they may be potential allelochemicals causing root-rot disease, and manifest as antagonists against probiotics in the rhizosphere soil of P. notoginseng.…”

Section: Discussionmentioning

confidence: 99%

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Allelochemicals of Panax notoginseng and their effects on various plants and rhizosphere microorganisms

Qiao

¹

,

Gu

²

,

Zhu

³

et al. 2020

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Panax notoginseng (Araliaceae) is an important ginseng herb with various health benefits and a history of cultivation in southwestern China over 400 years. In recent years P. notoginseng has faced serious continuous-cropping obstacles due to its large-scale cultivation. In this study, we aim to explore the allelochemicals of P. notoginseng and their interactions with various plants and rhizosphere microorganisms. The chemical constituents of the soil cultivated with 3-year-old P. notoginseng were studied by column chromatography, spectroscopic and GC-MS analyses. We identified 13 volatile components and isolated six triterpenes ( 1 – 4 , 6 – 7 ) and one anthraquinone ( 5 ). Compounds 1 – 7 were tested for their effects on seed germination and root elongation in P. notoginseng , corn, wheat, turnip, water spinach and Arabidopsis thaliana . We also examined the effect of compounds 1 – 7 on the growth of ten rhizosphere microorganisms of P. notoginseng . At a concentration of 1.0 μg mL −1 , compounds 3 and 5 – 7 caused the death of P. notoginseng root cells and compounds 2 , 6 and 7 induced the death of root cells of A. thaliana . Compounds 1 – 5 and 7 inhibited elongation of A. thaliana root tip cells at a concentration of 10.0 μg mL −1 . Moreover, at a concentration of 0.1 mg mL −1 , compounds 3 , 4 , 6 and 7 inhibited the growth of probiotics and promoted the growth of pathogens of P. notoginseng . These results suggest that these isolated ursane-type triterpenoid acids and anthraquinone are potential allelochemicals that contribute to continuous-cropping obstacles of P. notoginseng .

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“…Compounds 109 – 110 exhibited cytotoxic activity against the 293, HepG2, Huh7 and KB cell line with an ID 50 values from 0.024 to 0.62 µM. Further, studies of the cellular mechanism of compounds 109 – 110 showed that they arrested HepG2 cells at the S phase [ 61 ].…”

Section: Bioactive Compounds In Mangrove Plantsmentioning

confidence: 99%

Mangrove-Associated Fungi: A Novel Source of Potential Anticancer Compounds

Deshmukh

¹

,

Gupta

²

,

Prakash

³

et al. 2018

JoF

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Cancer is the second leading cause of death worldwide, and the number of cases is increasing alarmingly every year. Current research focuses on the development of novel chemotherapeutic drugs derived from natural as well as synthetic sources. The abundance and diversity in natural resources offer tremendous potential for the discovery of novel molecules with unique mechanisms for cancer therapy. Mangrove-derived fungi are rich source of novel metabolites, comprising novel structure classes with diverse biological activities. Across the globe, coastal areas are primarily dominated by mangrove forests, which offer an intensely complex environment and species that mostly remain unexplored. In recent years, many structurally diverse compounds with unique skeletons have been identified from mangrove fungi and evaluated for their antiproliferative properties. These compounds may serve as lead molecules for the development of new anticancer drugs. Mangrove endophytes can be modulated using epigenetic means or culture optimization methods to improve the yield or to produce various similar analogs. The present review provides an insight into the bioactive metabolites from mangrove endophytes reported during the period from 2012 to 2018 (up to April, 2018) along with their cytotoxic properties, focusing on their chemical structures and mode of action, as indicated in the literature.

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New and bioactive natural products from an endophyte of Panax notoginseng

Abstract: Secondary metabolites with cytotoxic activity, antiviral activity and antimicrobial activity from the endophytic fungi of Panax notoginseng.

Cited by 32 publications

References 47 publications

Two New Alkaloids from Fusarium tricinctum SYPF 7082, an Endophyte from the Root of Panax notoginseng

Two New Alkaloids from Fusarium tricinctum SYPF 7082, an Endophyte from the Root of Panax notoginseng

Allelochemicals of Panax notoginseng and their effects on various plants and rhizosphere microorganisms

Mangrove-Associated Fungi: A Novel Source of Potential Anticancer Compounds

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