New approach to immobilization and specific-sequence detection of nucleic acids based on poly(4-hydroxyphenylacetic acid)

Silva, Tatiana A.R.; Ferreira, Lucas Franco; Souza, Letícia Maria de; Goulart, Luiz Ricardo; Madurro, João M.; Brito‐Madurro, Ana G.

doi:10.1016/j.msec.2008.09.048

Cited by 27 publications

(22 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…PNAconjugated Ab has been immobilized on surfaces grafted with the antisense oligonucleotide or PNA by hybridization. 137,138 The ability to control surface-Ab distance through PNA monomers provides strong site specificity and orientation for Ab capturing.…”

Section: Site-specificmentioning

confidence: 99%

Immobilization of Antibodies and Enzymes on 3-Aminopropyltriethoxysilane-Functionalized Bioanalytical Platforms for Biosensors and Diagnostics

et al. 2014

View full text Add to dashboard Cite

/npsi/ctrl?lang=en http://nparc.cisti-icist.nrc-cnrc.gc.ca/npsi/ctrl?lang=fr READ THESE TERMS AND CONDITIONS CAREFULLY BEFORE USING THIS WEBSITE.http://nparc.cisti-icist.nrc-cnrc.gc.ca/npsi/jsp/nparc_cp.jsp?lang=en Vous avez des questions? Nous pouvons vous aider. Pour communiquer directement avec un auteur, consultez la première page de la revue dans laquelle son article a été publié afin de trouver ses coordonnées. Si vous n'arrivez pas à les repérer, communiquez avec nous à PublicationsArchive-ArchivesPublications@nrc-cnrc.gc.ca.

show abstract

Section: Site-specificmentioning

confidence: 99%

Immobilization of Antibodies and Enzymes on 3-Aminopropyltriethoxysilane-Functionalized Bioanalytical Platforms for Biosensors and Diagnostics

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Electrochemical devices based on DNA-mediated charge transport chemistry were widely reported [14,15]. Applications of electroactive polymers received considerable recent attention for DNA detection [16,17]. The use of polymers offers several advantages in the construction of DNA biosensors due to their cheapness and simple production.…”

Section: Introductionmentioning

confidence: 99%

Characterization of poly(vinylferrocenium) coated surfaces and their applications in DNA sensor technology

et al. 2010

View full text Add to dashboard Cite

Poly(vinylferrocenium) (PVF ? ) modified gold (Au) electrode was developed in this study for the electrochemical sensing of deoxyribonucleic acid (DNA) hybridization based on the oxidation signals of polymer and guanine, and also for the electrochemical investigation of interaction of anticancer drug, mitomycin C (MC) and DNA immobilized onto PVF ? modified Au electrode. PVF ? modified Au electrode was prepared by electrooxidation of poly(vinylferrocene) PVF at ?0.7 V versus Ag/AgCl reference electrode. The polymer modified electrode and DNA immobilized polymer modified electrode were characterized by X-ray photoelectron (XPS), Fourier transform infrared-attenuated total reflentance (FTIR-ATR) and alternating current (AC) impedance spectroscopy. For application studies, differential pulse voltammetry (DPV) technique was used.

show abstract

“…Nitrogenous bases spontaneously adsorb to some surfaces; however, Silva et al showed that GE, after being functionalized with polymeric films derived from hydroxyphenylacetic acid, maximizes the adsorption of these biomolecules [12,33]. The detection of the nitrogenous bases is more suitable in phosphate buffer and the Figure 6 shows the voltammetric behavior of a mixture of purine and pyrimidine bases immobilized on GE functionalized with poly-hydroxyphenylacetic acids in phosphate buffer at a pH of 7.00.…”

Section: Studies Of the Immobilization Of Nitrogenous Basesmentioning

confidence: 99%

“…These electrodes, after functionalization, have been used as platforms for the study of the hybridization of DNA nucleotides [6,11,12]. The classes of hydroxyphenylacetic acid polymer derivatives have been used to functionalize GE immunosensors [13,14].…”

Section: Introductionmentioning

confidence: 99%

Electropolymerization of hydroxyphenylacetic acid isomers and the development of a bioelectrode for the diagnosis of bacterial meningitis

Rodrigues

Ferreira

et al. 2015

J Appl Electrochem

Self Cite

View full text Add to dashboard Cite

In this work, three polymeric films derived from hydroxyphenylacetic acid isomers were electropolymerized onto the surfaces of graphite electrodes through cyclic voltammetry. Analysis of electrochemical behaviors and electrical properties of the three polymeric films were investigated and their probably structures were explored by molecular modeling. The best functionalization strategy for the incorporation and retention of adenine and guanine nitrogenous bases was the poly(3-hydroxyphenylacetic acid). It was applied for the immobilization of a nucleotide probe specific to DNA of the bacteria Neisseria meningitidis operated in amperometric and impedimetric indirect modes. The simple protocol of electrochemical detection of DNA by the bioelectrode, developed in this work, could be further enhanced and applied in a low-cost and pain-less platform to diagnose human meningitis infection.

show abstract

New approach to immobilization and specific-sequence detection of nucleic acids based on poly(4-hydroxyphenylacetic acid)

Cited by 27 publications

References 25 publications

Immobilization of Antibodies and Enzymes on 3-Aminopropyltriethoxysilane-Functionalized Bioanalytical Platforms for Biosensors and Diagnostics

Immobilization of Antibodies and Enzymes on 3-Aminopropyltriethoxysilane-Functionalized Bioanalytical Platforms for Biosensors and Diagnostics

Characterization of poly(vinylferrocenium) coated surfaces and their applications in DNA sensor technology

Electropolymerization of hydroxyphenylacetic acid isomers and the development of a bioelectrode for the diagnosis of bacterial meningitis

Contact Info

Product

Resources

About