New Aspects of Laboratory Techniques for Studies of Colorado Tick Fever

Indirect Hemagglutination for Diagnosis of Crimean Hemorrhagic Fever

“…The techniques for sensitization of erythrocytes, performance of the IHA test and in direct hemagglutination inhibition (IH1) test were similar to those described by us earlier for Colorado tick fever [2], In the IHA test, a 1-percent suspension of sensitized erythrocytes in a phosphate buffer (pH 7.2), containing 1 % horse serum, was added to dilutions of antigens commencing at 1:4 and increasing to 1:65,536. The results were scored after 2 h. In the IHI test, 4-8 units of the pretitrated antigen were mixed with 2-fold dilutions of sera (IAF or 0.1% y-globulin), and 20 min later the mixture was supplemented with sensitized eryth rocytes.…”

“…Sheep erythrocytes were pretreated with glutaraldehydc [11], Sensitization of erythro cytes was performed according to the technique described by Boyden [12] and modified by Rycaj [13], Some additional techniques for the performance of the reaction have been introduced by the authors [2]. For sensitization, 0.0125% y-globulin was used.…”

“…The indirect hemagglutination (IHA) test with antibody-sensitized eryth rocytes, based on the technique of Boyden, has been successfully used with certain viruses and Bedsonia: tick-borne encephalitis [1], Colorado tick fever [2], smallpox [3], and ornithosis [5], The purpose of the present work was to develop the IHA test and to apply it to serodiagnosis of Crimean hemorrhagic fever (CHF).…”

A Rapid Method for Detection of Bhanja Virus in Infected Ticks

“…The techniques of erythrocyte sensitization with immune y-globulins to Bhanja virus and of the IHA test with Bhanja virus were the same as described for Colorado tick fever [4,5]. Briefly, immune y-globulin isolated from immune ascitic fluid (IAF) by repeated precipitation with ethanol was used.…”

“…Following previous reports from this labo ratory on the value of the indirect hemaggluti nation (I HA) test for detection of arboviruses in various substrates [1,2], we have studied the possibilities and conditions for the appli cation of this test for a more rapid detection of Bhanja virus in experimentally infected Hyalomma asiaticum P. Sch. et E.Schl.…”