An indirect hemagglutination (IHA) test with Congo and Crimean hemorrhagic fever (CHF) viruses employing sheep erythrocytes sensitized with immune γ-globulin was developed. The titer of the IHA test with sucrose acetone antigens of the virus reached 1:16,000–1:32,000; the reaction was specifically inhibited in the presence of immune sera and sera of CHF-convalescent patients. High sensitivity and specificity of the reaction recommend it for the serodiagnostics of CHF.
A rapid method has been developed to detect Bhanja virus in experimentally infected Hyalomma asiatícum ticks by an indirect hemagglutination test. The titer of viral antigen in suspensions prepared from infected ticks ranged from 1:640 to 1:2,560; the antigen was demonstrated in both unfed and engorged ticks during an observation period from 2 weeks to 5 months after infection. The test is simple, easily reproduced, highly sensitive and specific, and may be useful for field studies with Bhanja virus.
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