New assay method for the determination of vinpocetine in human plasma by gas chromatography—mass spectrometry without transesterification caused by solvents: a reply

Hammes, Wilhelm; Weyhenmeyer, Roland

doi:10.1016/0378-4347(91)80159-a

Cited by 6 publications

(2 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The pharmacokinetic investigations of VP have been conducted by direct mass fragmentography [10,11], HPLC [12][13][14][15], sensitive enzyme immunoassay [16], gas chromatography (GC) with nitrogen-phosphorus detection [17][18][19][20][21] and GC-MS methods [22][23][24][25][26][27]. Also, several methods have been developed for the determination of AVA.…”

Section: Vinpocetinementioning

confidence: 99%

Determination of vinpocetine and its primary metabolite, apovincaminic acid, in rat plasma by liquid chromatography–tandem mass spectrometry

Xia

Guo

et al. 2010

Journal of Chromatography B

View full text Add to dashboard Cite

Section: Vinpocetinementioning

confidence: 99%

Determination of vinpocetine and its primary metabolite, apovincaminic acid, in rat plasma by liquid chromatography–tandem mass spectrometry

Xia

Guo

et al. 2010

Journal of Chromatography B

View full text Add to dashboard Cite

“…Several methods, such as GC [6], GC-MS [7,8], LC [9][10][11], LC-MS [5], and others [12] have been developed for separate determination of VP or AVA in plasma. To date, few studies on the simultaneous determination of VP and AVA in plasma by LS-MS-MS have been published [13,14].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Estimation of Vinpocetine and Its Metabolite, Apovincaminic Acid, in Beagle Plasma by LC-MS-MS

Yang

Lou

Gong³

et al. 2011

Chromatographia

View full text Add to dashboard Cite

A method was developed to determine vinpocetine and its metabolite, apovincaminic acid, in beagle plasma by LC-MS-MS. After protein precipitation with methanol, the supernatant of the sample was concentrated and injected into an Agilent Zorbax XDB-C 18 column. The sample was separated by a mobile phase consisting of acetonitrile and 0.2% formic acid solution, and the reading was determined on an Agilent 6410 Triple Quad Tandem mass spectrometer in multiple reaction monitoring mode with the following transitions: m/z 351.5 ? 280.2/266.3 for vinpocetine, 323.2 ? 236.1/280.2 for apovincaminic acid, and 411.2 ? 191.1 for the internal standard. The intra-and inter-day variances were less than 15% (RSD%), and average recoveries were higher than 80%. The linearity ranges (LR) between 0.1 and 20.0 ng mL -1 for vinpocetine (r 2 = 0.9980) and between 1.0 and 200.0 ng mL -1 for apovincaminic acid (r 2 = 0.9995) were established. In summary, this method is sensitive, specific, and appropriate for in vivo study of various dosage forms of vinpocetine.

show abstract