New bioactive metabolites from the elicited marine sponge-derived bacterium Actinokineospora spheciospongiae sp. nov.

Abstract

Several approaches have been dedicated to activate the cryptic gene clusters in the genomes of actinomycetes for the targeted discovery of new fascinating biomedical lead structures. In the current study, N-acetylglucosamine was used to maximize the chemical diversity of sponge-derived actinomycete Actinokineospora spheciospongiae sp. nov. HR–ESI–MS was employed for dereplication study and orthogonal partial least square-discriminant analysis was applied to evaluate the HR–ESI–MS data of the different fractions. As a result, two new fridamycins H (1) and I (2), along with three known compounds actinosporin C (3), D (4), and G (5) were isolated from the solid culture of sponge-associated actinomycete Actinokineospora spheciospongiae sp. nov., elicited with N-acetylglucosamine. Characterization of the isolated compounds was pursued using mass spectrometry and NMR spectral data. Fridamycin H (1) exhibited significant growth inhibitory activity towards Trypanosoma brucei strain TC221. These results highlight the potential of elicitation in sponge-associated actinomycetes as an effective strategy for the discovery of new anti-infective natural products.Electronic supplementary materialThe online version of this article (10.1186/s13568-018-0730-0) contains supplementary material, which is available to authorized users.

Natural Products Repertoire of the Red Sea

“…The alkaloids 44 and 45 exhibited cytotoxic activity against HeLa cells with IC 50 values of 5 and 4.5 µg/mL, respectively [71]. The dichloromethane extract of the sponge Dysidea cinereal, which was collected near Ras Zaatir in the Gulf of Eilat, yielded the avarol and avarone derivatives avarone A (46), 3 ,6 -dihydroxyavarone (47), avarol C (48) and avarone E (49) ( Figure 10). The avarone derivatives 47 and 49 showed activity as inhibitors of HIV-1 reverse transcriptase, with IC 50 values of 5 and 1 µg/mL, respectively.…”

“…Callyspongamide A (43) exhibited a cytotoxic activity against HeLa cells with an IC50 value of 4.1 μg/mL [70]. The two indole alkaloids hyrtioerectine B (44) and hyrtioerectine C (45) were isolated from the marine sponge Hyrtios erectus, avarol C (48) and avarone E (49) ( Figure 10). The avarone derivatives 47 and 49 showed activity as inhibitors of HIV-1 reverse transcriptase, with IC50 values of 5 and 1 μg/mL, respectively.…”

Actinomycetes from the Red Sea Sponge Coscinoderma mathewsi: Isolation, Diversity, and Potential for Bioactive Compounds Discovery

“…Positive and negative ESI ionization modes were utilized to carry out the high-resolution mass spectrometry coupled with a spray voltage at 4.5 kV, the capillary temperature at 320 • C, and mass range from m/z 150-1500. The MS dataset was processed and data were extracted using MZmine 2.20 based on the established parameters [48]. Mass ion peaks were detected and accompanied by chromatogram builder and chromatogram deconvolution.…”

“…This fight for survival may induce the synthesis of secondary metabolites that have a defending function against the other microorganism present in the culture medium, thus resulting in the silent activation of gene clusters. Another efficient method for inducing the production of cryptic secondary metabolites is the use of elicitors [48,67,68]. Elicitors are molecules that unregulate the expression of gene clusters involved in the biosynthesis of secondary metabolites in bacteria and fungi.…”

Saccharomonosporine A inspiration; synthesis of potent analogues as potential PIM kinase inhibitors

“…However, most of them are still in preclinical or early clinical development. 1 Renieramycin M extracted from the blue sponge Xestospongia sp. is the rst marine anticancer drug approved by the European Union and by the Food and Drug Administration (FDA) for the treatment of human colon, lung and brain cancer.…”