New cell attachment peptide sequences from conserved epitopes in the carboxy termini of fibrinogen

Gorodetsky, Raphael; Vexler, Akiva; Shamir, Meir; An, Jianqiang; Levdansky, Lilia; Shimeliovich, Irina; Marx, Gerard

doi:10.1016/s0014-4827(03)00120-4

Cited by 39 publications

(44 citation statements)

References 45 publications

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“…[29][30][31][32] The mechanism of cells binding to this fibrin-based matrix [24][25] may be at least partially attributed to the exposure of haptides, new cell-binding epitopes on fibrinogen that selectively adhere to matrix-dependent cells. 34 Fibrin microbeads could be used for the isolation of MSC, for their expansion and differentiation. We previously reported that FMB could separate MSC from a suspension of mixed cell types with high efficiency.…”

Section: Discussionmentioning

confidence: 99%

Isolation of mesenchymal stem cells from G-CSF-mobilized human peripheral blood using fibrin microbeads

Kassis

Zangi

Rivkin

et al. 2006

Bone Marrow Transplant

226

168

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Adult mesenchymal stem cells (MSC) that are able to differentiate into various mesenchymal cell types are typically isolated from bone marrow, but their significant presence in human peripheral blood (PB) is controversial. Fibrin microbeads (FMB) that bind matrix-dependent cells were used to isolate MSC from the mononuclear fraction of mobilized PB of adult healthy human donors treated with a granulocyte colony-stimulating factor. Isolation by plastic adherence resulted in a negligible number of MSC in all samples tested, whereas FMBbased isolation yielded spindle-shaped cell samples that could further expand on plastic or on FMB in eight out of the 11 samples. The yield of these cells at days 17-18 after the harvest was B0.5% of the initial cell number. The isolated cells were grown on plastic and characterized by FACS analysis and immunohistochemistry for specific markers. Following culturing and first passage, the FMBisolated cells stained positive for mesenchymal stromal cell markers CD90 and CD105, expressed vimentin and fibronectin and were negative for hematopoietic markers CD45 and CD34. These cells could differentiate into osteoblasts, adipocytes and chondrocytes. This study indicates that FMB may have special advantage in isolating MSC from sources such as mobilized PB, where the number of such cells is scarce.

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Section: Discussionmentioning

confidence: 99%

Isolation of mesenchymal stem cells from G-CSF-mobilized human peripheral blood using fibrin microbeads

Kassis

Zangi

Rivkin

et al. 2006

Bone Marrow Transplant

226

168

View full text Add to dashboard Cite

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“…We specifically sought to compare the response of MSCs to HP following encapsulation in fibrin, where cells are known to directly adhere and spread within the hydrogel [21,22], to that in agarose, where cells do not adhere and hence maintain a spherical morphology when encapsulated within the hydrogel [23][24][25]. Our hypothesis was that cells seeded in fibrin hydrogels would be more mechanosensitive and hence demonstrate a more robust response to HP, while cells seeded in agarose hydrogels would show little to no response to HP.…”

Section: Introductionmentioning

confidence: 99%

Cell–matrix interactions regulate mesenchymal stem cell response to hydrostatic pressure

et al. 2012

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“…For instance, fibrin-related Haptides that are released as a result of pathological cardiovascular events may adversely affect cardiac function. Previous studies revealed that synthetic Haptides dissolved as a monomeric form in aqueous solution penetrated and accumulated in different cell types, including fibroblasts, and endothelial and smooth muscle cells [13]. With the elevation of their concentrations they created nanoaggregates, which interacted with membranes and rapidly penetrated into the cells at nonsaturable kinetics and even enhanced penetration of liposomal compositions into them, acting as classic cell-penetrating peptides [12,13].…”

Section: Discussionmentioning

confidence: 99%

“…data). These peptides were termed ‘Haptides’ because of their haptotactic (cell-binding) properties [12,13]. Haptides were initially studied as cell-binding domains on fibrin(ogen), which are not necessarily mediated by integrins [14,15].…”

Section: Introductionmentioning

confidence: 99%

“…Haptides were initially studied as cell-binding domains on fibrin(ogen), which are not necessarily mediated by integrins [14,15]. The Haptides are relatively rich in both basic amino acids as well as cyclic hydrophobic groups, and are rapidly internalized into cells and can promote liposome uptake at nonsaturable kinetics, with no apparent cytotoxic effect [12,13]. …”

Section: Introductionmentioning

confidence: 99%

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Short Homologous Peptides Based on C-Terminal Sequences of Fibrinogen β- and γ-Chains (Haptides) Affect Cardiovascular Function by eNOS Inhibition

Basheer

Schwalb²,

Gilon³

et al. 2010

J Vasc Res

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Haptides are a family of 19–21-mer cell-binding and permeating peptides homologous to sequences in the C termini on both fibrinogen β- and γ-chain (Cβ and preCγ, respectively). The effect of the Haptides on the cardiovascular system was studied by different assays, including the activity of isolated perfused rat heart and blood vessels in the organ bath. Haptides (50–80 µg/ml) decreased the hemodynamic functions of perfused rat hearts by up to 60% (p < 0.05) in a dose-dependent manner. Whole fibrinogen or a control nonrelated peptide (Cα) did not show such an effect. The NO donor, sodium nitroprusside, reversed the inhibitory effects of Haptides. L-NAME, an endothelial nitric oxide synthase (eNOS) inhibitor, did not further augment the effect of the Haptides. Perfused ^FITCHaptides were attached to the coronary endothelium. In myocardial homogenates and HUVEC, Haptides significantly decreased eNOS activity, but had no effect on the contraction of isolated cultured adult cardiomyocytes. Haptides also significantly enhanced the contraction of rings of rat aorta and human mammary artery vessels ex vivo only when the endothelium was intact. Haptides seem to affect the coronary endothelium, but not the cardiomyocytes, by inhibiting eNOS activity, causing vasoconstriction, temporary ischemia and impaired myocardial function that seem to be related to the amino acid composition of the Haptides.

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New cell attachment peptide sequences from conserved epitopes in the carboxy termini of fibrinogen

Cited by 39 publications

References 45 publications

Isolation of mesenchymal stem cells from G-CSF-mobilized human peripheral blood using fibrin microbeads

Isolation of mesenchymal stem cells from G-CSF-mobilized human peripheral blood using fibrin microbeads

Cell–matrix interactions regulate mesenchymal stem cell response to hydrostatic pressure

Short Homologous Peptides Based on C-Terminal Sequences of Fibrinogen β- and γ-Chains (Haptides) Affect Cardiovascular Function by eNOS Inhibition

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