New Functional Amorphous Calcium Phosphate Nanocomposites by Enzyme-Assisted Biomineralization

Abstract

In the present study, we report on enzyme-assisted formation of biomineralized amorphous calcium phosphate nanocomposites (ACP-NCs). About 100-200 nm sizes of the spherical porous enzyme-assisted ACP-NCs were successfully synthesized via double reverse microemulsion, but no ACP-NCs formed without the enzyme. It is believed that the enzyme was used as an organic template or additive that could regulate the biomineralization process. The enzyme-assisted ACP-NCs were well characterized by X-ray diffraction, transmission electron microscopy, scanning electron microscopy, dynamic light scattering, and Brunauer-Emmett-Teller (BET) criteria. The BET surface area, total pore volume, pore size from adsorption, and pore size from desorption of the ACP-NCs were 163 m(2) g(-1) or 0.37 cm(3) g(-1), 8.87 nm, and 7.48 nm, respectively. The enzyme-assisted ACP-NCs retained about 43% of the catalytic activity of free carboxyl esterase. Furthermore, they preserved their bioactivity even after the 10th reuse and were stable over 10 days even under a stringent shaking conditions. The reported method paves the way for novel biomineralization via enzyme molecules to form functional enzymes containing nanocomposites.

Enzymatic Mineralization of Silk Scaffolds

“…Biomineralization is the natural process by which minerals are formed by organisms including proteins, peptides, and enzymes . Most of these mineralized products are composite materials comprised of organic and mineral components.…”

Evaluation of Amorphous Calcium Phosphate as an Advantageous Solid-Phase Extraction Adsorbent for Analysis of Oxalic Acid in Plant Xylem Saps by Rp-HPLC

“…[19] This result was in good agreement with previous work. [20] The synthesized ACP was used as the adsorbent to separate oxalic acid from xylem sap samples.…”

Yolk @ cage-Shell Hollow Mesoporous Monodispersion Nanospheres of Amorphous Calcium Phosphate for Drug Delivery with High Loading Capacity

“…Among the different morphological nanostructures, yolk-shell hollow spheres with porosity [25] are more advantageous for applications in biomedical fields such as loading drug, protein or DNA molecules, due to their different specific surface areas and morphologies [26, 27]. However, the reports about yolk-shell calcium phosphate particles are very little, and the particles reported previously could not meet the loading requires because their big yolk size compared with the shell, which results in the smaller interior space and low loading capacity [25].…”