New Gene for Bacterial Blight Resistance in Rice Located on Chromosome 12 Identified from Minghui 63, an Elite Restorer Line

Abstract

Bacterial blight, caused by Xanthomonas oryzae pv. oryzae, is a serious disease of rice worldwide. A new dominant gene for bacterial blight resistance in rice, Xa25(t), was identified from Minghui 63, a restorer line for a number of rice hybrids that are widely cultivated in China. This gene conferred resistance to Philippine race 9 (PXO339) of X. oryzae pv. oryzae in both seedling and adult stages. It was mapped to the centromeric region of chromosome 12, 2.5 cM from a disease resistance gene-homologous sequence, NBS109, and 7.3 cM from a restriction fragment length polymorphism marker, G1314. The genomic location of this gene is similar to the previously identified blast resistance genes, Pi-ta and Pi-ta2.

The Expression Pattern of a Rice Disease Resistance Gene Xa3/Xa26 Is Differentially Regulated by the Genetic Backgrounds and Developmental Stages That Influence Its Function

“…This dosage-dependent developmental control may also be related to bacterial recognition specificity. Minghui 63 was highly resistant to Xoo strain JL691 at both seedling and adult stages (Chen et al 2002), suggesting that XA3 can efficiently and specifically perceive the effector of JL691 and that more XA3 proteins are required for recognition of PXO61. R genes often express constitutively in either uninfected or infected plants (De Ilarduya and Kaloshian 2001;Shen et al 2002;Paal et al 2004;Huang et al 2005;Schornack et al 2005), which agrees with their common role in pathogen recognition.…”

“…In addition, different indica backgrounds also influence the function of R genes. Minghui 63 carries another bacterial blight resistance gene, Xa25(t), in addition to Xa26 (Chen et al 2002). Another indica rice line IRBB3 is well known to carry only one R gene, Xa3, for Xoo resistance (Ogawa et al 1991).…”

The Coiled-Coil and Nucleotide Binding Domains of BROWN PLANTHOPPER RESISTANCE14 Function in Signaling and Resistance against Planthopper in Rice

“…To examine bacterial blight disease resistance, wild-type and transgenic rice plants at the four-leaf stage were inoculated with the bacterial pathogen Xanthomonas oryzae (strain PXO99) using the leaf-clipping method (Chen et al, 2002). The inoculated plants were grown and examined under natural field conditions during the normal rice growing season from June to August at the Experimental Station of Wuhan University, Wuhan, China.…”

Two Different Transcripts of a LAMMER Kinase Gene Play Opposite Roles in Disease Resistance

“…To evaluate bacterial blight disease, plants were inoculated with Xoo at the booting (panicle development) stage by the leaf-clipping method (Chen et al, 2002), and disease was scored by measuring the percentage of the lesion area (lesion length/leaf length) at 11 to 15 d after inoculation. Xoo growth rate in rice leaves was determined by counting colony-forming units (Sun et al, 2004).…”