New Genus of the Actinomycetales: Kibdelosporangium aridum gen. nov., sp. nov.

Shearer, Marcia C.; Colman, Paula M.; Ferrin, Rhonda M.; Nisbet, Louis J.; Nash, C. H.

doi:10.1099/00207713-36-1-47

Cited by 45 publications

(31 citation statements)

References 12 publications

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“…The family currently consists of 14 genera with validly published names: Actinoalloteichus (Tamura et al, 2000), Actinopolyspora (Gochnauer et al, 1975), Amycolatopsis (Lechevalier et al, 1986), Crossiella (Labeda, 2001), Goodfellowia (Labeda & Kroppenstedt, 2006), Kibdelosporangium (Shearer et al, 1986), Kutzneria (Stackebrandt et al, 1994), Prauserella (Kim & Goodfellow, 1999), Pseudonocardia (Henssen, 1957), Saccharomonospora (Nonomura & Ohara, 1971), Saccharopolyspora (Lacey & Goodfellow, 1975), Streptoalloteichus (Tomita et al, 1987), Thermobispora (Wang et al, 1996) and Thermocrispum (Korn-Wendisch et al, 1995). Strain YIM 0006 T was isolated during an investigation of actinomycete diversity in soil from a tropical rainforest in Chiang Mai, in northern Thailand.…”

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Actinomycetospora chiangmaiensis gen. nov., sp. nov., a new member of the family Pseudonocardiaceae

Jiang

Wiese

Tang

et al. 2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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A novel actinomycete strain, YIM 0006 T , was isolated from soil of a tropical rainforest in northern Thailand. The isolate displayed the following characteristics: aerial mycelium is absent, short spore chains are formed directly on the substrate mycelium, contains meso-diaminopimelic acid, arabinose and galactose (cell-wall chemotype IV), the diagnostic phospholipid is phosphatidylcholine, MK-9(H 4 ) is the predominant menaquinone and the G+C content of the genomic DNA is 69.0 mol%. Phylogenetic analysis and phenotypic characteristics showed that strain YIM 0006 T belongs to the family Pseudonocardiaceae but can be distinguished from representatives of all genera classified in the family. The novel genus and species Actinomycetospora chiangmaiensis gen. nov., sp. nov. are proposed, with strain YIM 0006 T (5CCTCC AA 205017 T 5DSM 45062 T ) as the type strain of Actinomycetospora chiangmaiensis.The first description of the family Pseudonocardiaceae was given by Embley et al. (1988), and the description was emended by Stackebrandt et al. (1997) et al., 1996) and Thermocrispum (Korn-Wendisch et al., 1995). Strain YIM 0006 T was isolated during an investigation of actinomycete diversity in soil from a tropical rainforest in Chiang Mai, in northern Thailand. Here, we report on the classification and characterization of strain YIM 0006 T and propose a novel genus and species of the family Pseudonocardiaceae to accommodate the strain.Strain YIM 0006 T was isolated from a soil sample after 2 weeks incubation at 28 u C on starch-glycerol medium as described by Jiang et al. (2006). Cultural characteristics of the strain were determined after growth at 28 u C for 2 weeks by methods used in the International Streptomyces Project (ISP; Shirling & Gottlieb, 1966) as well as by using Czapek's medium and nutrient agar (Dong & Cai, 2001). Colour determination was performed with colour chips from the ISCC-NBS Color Charts Standard Samples no. 2106 (Kelly, 1964). Morphological observations of spore chains and mycelia were made by light microscopy (Olympus microscope BH-2) and scanning electron microscopy (Philips XL30 ESEM-TMP) after 20-50 days incubation. Gram staining (Hucker's modification; Society for American Bacteriologists, 1957) and Ziehl-Neelsen preparations (Gordon, 1967) were evaluated by light microscopy.Growth of strain YIM 0006 T was poor on most media tested, although the strain grew well but slowly on yeast extractmalt extract agar (ISP 2). Soluble pigments were not produced under any conditions tested in this study. Aerial mycelium was not observed on any of the tested media. The vegetative mycelium fragmented into rod-shaped elements (Fig. 1a) and was pale to brilliant orange-yellow in colour. Short spore chains were formed directly on the vegetative mycelium (Fig. 1a, b). The strain displayed bud-like structures of the spore chains, as has also been described for members of the genus Pseudonocardia (Huang et al., 2002). Spores were short and rod-shaped, 0.3-0.660.8-1.2 mm. The spore surface was smooth.The...

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“…Data for reference genera were derived from Shearer et al (1986), Mertz & Yao (1988), Tomita et al (1993), Henssen (1957), Huang et al (2002), Reichert et al (1998) and Kämpfer et al (2006).…”

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Actinomycetospora chiangmaiensis gen. nov., sp. nov., a new member of the family Pseudonocardiaceae

Jiang

Wiese

Tang

et al. 2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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“…The family Pseudonocardiaceae was established by Embley et al (1988), and emended by Stackebrandt et al (1997) based on 16S rRNA gene sequence analysis, and contained the type genus Pseudonocardia (Henssen,1957) and the genera Actinopolyspora (Gochnauer et al, 1975), Actinosynnema (Hasegawa et al, 1978), Amycolatopsis (Lechevalier et al, 1986), Kibdelosporangium (Shearer et al, 1986), Kutzneria (Stackebrandt et al, 1994), Lentzea (Yassin et al, 1995), Saccharomonospora (Nonomura & Ohara, 1971), Saccharopolyspora (Lacey & Goodfellow, 1975), Saccharothrix (Labeda et al, 1984), Streptoalloteichus (Tomita et al, 1978) and Thermocrispum (Korn-Wendisch et al, 1995). Subsequently, 14 genera have been added to family, Prauserella (Kim & Goodfellow 1999), Actinoalloteichus (Tamura et al, 2000), Crossiella (Labeda, 2001), Actinomycetospora (Jiang et al, 2008), Allokutzneria , Goodfellowiella , Sciscionella (Tian et al, 2009), Actinophytocola (Indananda et al, 2010), Alloactinosynnema (Yuan et al, 2010), Haloechinothrix (Tang et al, 2010), Yuhushiella (Mao et al, 2011) and Labedaea (Lee, 2012).…”

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Thermotunica guangxiensis gen. nov., sp. nov., isolated from mushroom residue compost

Yunpeng

Liu

et al. 2014

International Journal of Systematic and Evolutionary Microbiology

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A novel thermophilic actinomycete, designated AG2-7 T , was isolated from mushroom residue compost in Guangxi University, Nanning, China. The strain grew optimally at 45-60 6C, at pH 7.0 and with 0-3.0 % (w/v) NaCl. Vegetative mycelia were branched and whitish to pale yellow without fragmentation. Aerial mycelium was abundant, whitish and differentiated into long chains of spores, with a membranous structure or tunica partially covering the surface of aerial hyphae. The non-motile spores were oval in shape with a ridged surface. Strain T contained mesodiaminopimelic acid as the diagnostic diamino acid, and the whole-cell sugars were galactose and ribose. Major fatty acids were iso-C 16 : 0 (27.51 %), iso-C 17 : 0 (10.47 %) and anteiso-C 17 : 0 (12.01 %). MK-9(H 4 ) was the predominant menaquinone. The polar phospholipids were diphosphatidylglycerol, ninhydrin-positive glycophospholipid, phosphatidylinositol, phosphatidylinositol mannoside, phosphatidylethanolamine, phosphatidylmethylethanolamine, an unknown phospholipid and unknown glucosamine-containing phospholipids. The G+C content of the genomic DNA was 63.6 mol%. 16S rRNA gene sequence analysis showed that the organism belonged to the family Pseudonocardiaceae, suborder Pseudonocardineae and showed more than 5 % divergence from other members of the family. Based on the phenotypic and phylogenetic data, strain AG2-7 T represents a novel species of a new genus in the family Pseudonocardiaceae, for which the name Thermotunica guangxiensis gen. nov., sp. nov. is proposed. The type strain of the type species is AG2-7 T (5ATCC BAA-2499 T 5CGMCC T ).The family Pseudonocardiaceae was established by Embley et al. (1988) (Hayakawa & Nonomura, 1987) and incubation at 50 u C in the dark for 3 days under aerobic conditions. The isolate was maintained on modified Sauton's agar (Mordarska et al., 1972) at 4 u C and as suspensions of mycelial fragments in 20 % (v/v) glycerol at 220 u C.Cultural characteristics of strain AG2-7 T were tested on ISP2, ISP3, ISP4, ISP5, ISP6 and ISP7 media (Shirling & Gottlieb, 1966), Gause's asparagine agar (Gause et al., 1983), Bennett's agar (Jones, 1949), Czapek solution agar, potato agar (Waksman, 1961), Sauton's agar, HV medium and water agar (15.0 g agar, 1 l tap water) after incubation for 7 days at 50 u C. Morphological characteristics were observed under a light microscope (80i; Nikon) and a scanning electron microscope (VEGA3 SBU; TESCAN) after incubation for 3-7 days on HV media at 50 u C. Growth temperature and resistance to NaCl was tested on modified Bennett's agar at 4, 16, 28, 37, 40, 45, 50, 55, 60, 65 and 70 u C and observed after 7 and 14 days. Tolerance to NaCl between 0 and 15 % (at intervals of 1 %) was read after 3 and 7 days at 50 u C. The pH range and the optimum pH for growth were examined on Sauton's agar with the pH range between pH 4.0 and 11.0 (in intervals of 1.0 pH unit) using the following buffer systems: for pH 4.0-6.0, 0.1 M citric acid/0.1 M sodium citrate; pH 7.0-9.0, 0.1 M Tris/0.1 M HCl; pH 9.5-10.0, 0...

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“…On: Sat, 12 May 2018 11:25:41 (previous illegitimate name Goodfellowia , Kibdelosporangium (Shearer et al, 1986), Kutzneria (Stackebrandt et al, 1994), Prauserella (Kim & Goodfellow, 1999), Pseudonocardia (Henssen, 1957), Saccharomonospora (Nonomura & Ohara, 1971), Saccharopolyspora (Lacey & Goodfellow, 1975), Sciscionella (Tian et al 2009), Streptoalloteichus (Tomita et al, 1978) and Thermocrispum (Korn-Wendisch et al, 1995). Recently, Zhi et al (2009) proposed a new suborder Actinopolysporineae and family Actinopolysporaceae to accommodate the genus Actinopolyspora and assigned the genus Thermobispora to the suborder Streptosporangineae, although the family-level affiliation of the latter genus remains uncertain.…”

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confidence: 99%

Yuhushiella deserti gen. nov., sp. nov., a new member of the suborder Pseudonocardineae

Mao

Wang

Dai

et al. 2011

International Journal of Systematic and Evolutionary Microbiology

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A thermotolerant, Gram-stain-positive, aerobic, sporangium-forming actinomycete, strain RA45T, was isolated from a desert region in Xinjiang Uigur Autonomous Region, north-western China. Comparative analysis of the 16S rRNA gene sequence and phenotypic characterization revealed that strain RA45T belonged phylogenetically to the family Pseudonocardiaceae of the suborder Pseudonocardineae. Strain RA45T showed more than 5 % 16S rRNA gene sequence divergence from recognized species of genera in the family Pseudonocardiaceae, forming a distinct lineage within the evolutionary radiation occupied by the genera Amycolatopsis, Prauserella, Thermocrispum, Saccharomonospora, Saccharopolyspora and Sciscionella, but distinct from each of them. The affiliation to the family was supported by the presence of suborder- and family-specific 16S rRNA signature nucleotides, a DNA G+C content of 69.9 mol%, the presence of meso-diaminopimelic acid, ribose, arabinose, glucose and galactose, which are characteristic components of cell-wall chemotype IV of actinomycetes, the presence of menaquinone MK-9(H4) as the major respiratory lipoquinone, a lack of mycolic acids and the presence of an N-acetylated type of muramic acid. However, strain RA45T differed from known genera of the family in its polar lipid composition: the major phospholipids were phosphatidylethanolamine, phosphatidylinositol mannosides, phosphatidylmethylethanolamine, diphosphatidylglycerol, phospholipids of unknown structure and phospholipids of unknown structure containing glucosamine (phospholipid type IV). Based on its morphological, chemotaxonomic and phylogenetic characteristics, strain RA45T is considered to represent a novel species of a new genus in the family Pseudonocardiaceae, for which the name Yuhushiella deserti gen. nov., sp. nov. is proposed. The type strain of Yuhushiella deserti is RA45T (=CGMCC 4.5579T =JCM 16584T).

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New Genus of the Actinomycetales: Kibdelosporangium aridum gen. nov., sp. nov.

Cited by 45 publications

References 12 publications

Actinomycetospora chiangmaiensis gen. nov., sp. nov., a new member of the family Pseudonocardiaceae

Actinomycetospora chiangmaiensis gen. nov., sp. nov., a new member of the family Pseudonocardiaceae

Thermotunica guangxiensis gen. nov., sp. nov., isolated from mushroom residue compost

Yuhushiella deserti gen. nov., sp. nov., a new member of the suborder Pseudonocardineae

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