New high-performance liquid chromatography-dad method for analytical determination of arbutin and hydroquinone in rat plasma

Gallo, Francesca Romana; Pagliuca, Giordana; Multari, Giuseppina; Panzini, Gianluca; D’Amore, Emanuela; Altieri, Ilaria

doi:10.4103/0250-474x.169031

Cited by 19 publications

(1 citation statement)

References 11 publications

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“…Origanum majorana L. is a species capable of synthesizing arbutin, a polyphenol widely used in the cosmetics industry for the treatment of hyperpigmentation disorders without causing melanocytoxicity; thus, it is an option for replacing the use of hydroquinone and corticosteroids (Cala et al 2021;Lukas et al 2010;Zhu and Gao 2008). Arbutin is a hydroquinone glycoside and is one of the main constituents of the Arctostaphylos uva-ursi plant and is found in signi cant amounts in Origanum majorana (Gallo et al 2015;Saeedi et al 2021). This compound is widely used to lighten the skin in cosmetic products due to its property of suppressing melanin biosynthesis in human skin (Boo 2021;Saeedi et al 2021).…”

Section: Introductionmentioning

confidence: 99%

Impact of photon flux density and light spectral quality on biomass production and arbutin compound accumulation in Origanum majorana L. plantlets

Cossa,

Rocha,

de Assis

et al. 2023

Plant Cell Tiss Organ Cult

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Light is one of the factors that in uence the growth, development and synthesis of secondary metabolites of different plant species in vitro. Origanum majorana is an aromatic plant belonging to a group of plants that produce arbutin, a substance of importance in the cosmetic industry. The objective of this study was to verify the effect of light intensity and wavelength on the growth and accumulation of arbutin in vitro in plantlets. Nodal segments were grown in MS culture medium under photon ux densities of 26, 51, 69, 94 and 130 µmol m − 2 s − 1 . In the light spectrum, nodal segments were grown under different light-emitting diodes LED: red; blue; a combination of blue and red light (30% B:70% R; 50% B:50% R; 70% B:30% R); and white, yellow and green. After 40 days, the growth, photosynthetic pigments and arbutin accumulation were evaluated by HPLC-DAD. The lowest light intensity (26 µmol m − 2 s − 1 ) provided a higher content of photosynthetic pigments, and the intensity of 94 µmol m − 2 s − 1 increased dry biomass accumulation. Greater accumulation of arbutin was observed under a higher light intensity of 130 µmol m − 2 s − 1 . Regarding the quality of light, different wavelengths did not signi cantly affect the number of shoots or shoot length. The white light spectrum stimulated a greater accumulation of dry biomass. The green light provided a greater accumulation of photosynthetic pigments. The amount of arbutin was in uenced by the different light spectra. The red spectrum promoted the highest arbutin content, and the yellow inhibited.

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Section: Introductionmentioning

confidence: 99%

Impact of photon flux density and light spectral quality on biomass production and arbutin compound accumulation in Origanum majorana L. plantlets

Cossa,

Rocha,

de Assis

et al. 2023

Plant Cell Tiss Organ Cult

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Comparative pharmacokinetics of four major flavonoids in normal and chronic gastritis rats after oral administration of different combinations of Banxia Xiexin decoction

Zhou

Liu

et al. 2022

Biomedical Chromatography

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Chronic gastritis (CG) has become a major threat to human health. Banxia Xiexin Decoction (BXXXD) has been used clinically to treat gastritis by acting on the spleen and stomach for thousands of years. Baicalin, wogonoside, liquiritin and liquiritigenin are the main bioactive flavonoids of BXXXD. A rapid, sensitive and selective HPLCtriple quadrupole (TQ)-MS/MS method was developed to simultaneously quantify the four flavonoids in rat plasma in this study. With salidroside as internal standard (IS), plasma samples were extracted and separated on a Welch HPLC XB-C 18 column (2.1 Â 50 mm, 1.8 μm) using gradient elution. The optimized gradient of the mobile phase consisted of water (containing 0.1% formic acid) (A) and methanol (B) was used.Detection was implemented in multiple reaction monitoring mode with an electrospray negative ionization source. The comparative pharmacokinetics of four analytes in normal and CG rats after oral administration of BXXXD or its different compatibilities were first investigated. The results indicated that the pharmacokinetic behaviors of analytes were obviously changed in CG rats. From the comparison between the whole prescription group and the compatibility groups, it was found that the pharmacokinetic behavior of analytes also changed to some extent. The pharmacokinetic alterations of analytes might be due to the pathological conditions of CG.

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Development and validation of a modified QuEChERS method coupled with LC-MS/MS to determine arbutin in pear peels

Kim

Lee

2016

Food Sci Biotechnol

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A new effective method was developed to determine the concentration of arbutin in pear peels using a modified QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) method and liquid chromatography-tandem mass spectrometry (LC-MS/MS). The original QuEChERS was modified to enable the extraction of the polar arbutin molecule. Use of an initial 50:50 acetonitrile:water extraction solvent led to the highest extraction efficiency. The arbutin extracted from pear peels was found to be identical to the β-arbutin standard, as confirmed by NMR and LC-MS/MS analyses. For quantitative analysis, the mass spectra of the precursor ion [M+NH] at / 290.0 and the product ion of arbutin at / 163.0 were used. The limit of detection, limit of quantification, linearity, precision, accuracy, and recovery of the proposed method were evaluated. We successfully applied this method to pear samples and it may be suitable for the quantitative analysis of arbutin in other similar plant materials.

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New high-performance liquid chromatography-dad method for analytical determination of arbutin and hydroquinone in rat plasma

Cited by 19 publications

References 11 publications

Impact of photon flux density and light spectral quality on biomass production and arbutin compound accumulation in Origanum majorana L. plantlets

Impact of photon flux density and light spectral quality on biomass production and arbutin compound accumulation in Origanum majorana L. plantlets

Comparative pharmacokinetics of four major flavonoids in normal and chronic gastritis rats after oral administration of different combinations of Banxia Xiexin decoction

Development and validation of a modified QuEChERS method coupled with LC-MS/MS to determine arbutin in pear peels

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