New insights into the emerging role of oral spirochaetes in periodontal disease

Visser, Michelle B.; Ellen, Richard P.

doi:10.1111/j.1469-0691.2011.03460.x

Cited by 65 publications

(58 citation statements)

References 149 publications

(179 reference statements)

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“…micra, and adherence levels to F. nucleatum similar to those of T. denticola. These results provide an in vitro basis for the findings that T. denticola is almost always found associated with P. gingivalis in vivo, while T. vincentii is isolated from subgingival microbial communities (Siqueira & Rôças, 2004;Visser & Ellen, 2011) but not necessarily associated with P. gingivalis or with periodontal disease. Overall, T. vincentii appeared to have a less adherent phenotype than T. denticola.…”

Section: Discussionmentioning

confidence: 87%

“…Spirochaetes are detected in most samples of subgingival plaque microbiota taken from subjects with a range of periodontal conditions (Paster et al, 2001;Colombo et al, 2009;Visser & Ellen, 2011). T. denticola has been the most frequently isolated and best-characterized oral spirochaete (Moore et al, 1982;Riviere et al, 1992), but it is evident that multiple phylotypes are found in individual subjects (Dewhirst et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

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Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities

et al. 2012

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Section: Discussionmentioning

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities

et al. 2012

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“…Thus, the reduced virulence can be due to the synergistic effects of defective motility, chemotaxis, and biofilm formation in the Td⌬214 mutants. T. denticola produces numerous virulence factors involved in cytotoxicity, adherence, invasion, and immunomodulation (38,39). Alternatively, TDE0214 may also affect the pathogenicity of T. denticola via other virulence factors.…”

Section: Discussionmentioning

confidence: 99%

Inactivation of Cyclic Di-GMP Binding Protein TDE0214 Affects the Motility, Biofilm Formation, and Virulence of Treponema denticola

Bian

et al. 2013

Journal of Bacteriology

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bAs a ubiquitous second messenger, cyclic dimeric GMP (c-di-GMP) has been studied in numerous bacteria. The oral spirochete Treponema denticola, a periodontal pathogen associated with human periodontitis, has a complex c-di-GMP signaling network. However, its function remains unexplored. In this report, a PilZ-like c-di-GMP binding protein (TDE0214) was studied to investigate the role of c-di-GMP in the spirochete. TDE0214 harbors a PilZ domain with two signature motifs: RXXXR and DXSXXG. Biochemical studies showed that TDE0214 binds c-di-GMP in a specific manner, with a dissociation constant (K d ) value of 1.73 M, which is in the low range compared to those of other reported c-di-GMP binding proteins. To reveal the role of c-di-GMP in T. denticola, a TDE0214 deletion mutant (Td⌬214) was constructed and analyzed in detail. First, swim plate and single-cell tracking analyses showed that Td⌬214 had abnormal swimming behaviors: the mutant was less motile and reversed more frequently than the wild type. Second, we found that biofilm formation of Td⌬214 was substantially repressed (ϳ6.0-fold reduction). Finally, in vivo studies using a mouse skin abscess model revealed that the invasiveness and ability to induce skin abscesses and host humoral immune responses were significantly attenuated in Td⌬214, indicative of the impact that TDE0214 has on the virulence of T. denticola. Collectively, the results reported here indicate that TDE0214 plays important roles in motility, biofilm formation, and virulence of the spirochete. This report also paves a way to further unveil the roles of the c-di-GMP signaling network in the biology and pathogenicity of T. denticola.

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“…These organisms are among the last few as-yet-uncultured major human pathogens. Similarly, among the numerous Treponema phylotypes found in the oral cavity, only 10 different species have been cultivated, with Treponema denticola being the most extensively studied (122). The development of an axenic culture of pathogenic Treponema remains a challenge for microbiologists.…”

Section: Spirochetesmentioning

confidence: 99%

Current and Past Strategies for Bacterial Culture in Clinical Microbiology

et al. 2015

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SUMMARY A pure bacterial culture remains essential for the study of its virulence, its antibiotic susceptibility, and its genome sequence in order to facilitate the understanding and treatment of caused diseases. The first culture conditions empirically varied incubation time, nutrients, atmosphere, and temperature; culture was then gradually abandoned in favor of molecular methods. The rebirth of culture in clinical microbiology was prompted by microbiologists specializing in intracellular bacteria. The shell vial procedure allowed the culture of new species of Rickettsia . The design of axenic media for growing fastidious bacteria such as Tropheryma whipplei and Coxiella burnetii and the ability of amoebal coculture to discover new bacteria constituted major advances. Strong efforts associating optimized culture media, detection methods, and a microaerophilic atmosphere allowed a dramatic decrease of the time of Mycobacterium tuberculosis culture. The use of a new versatile medium allowed an extension of the repertoire of archaea. Finally, to optimize the culture of anaerobes in routine bacteriology laboratories, the addition of antioxidants in culture media under an aerobic atmosphere allowed the growth of strictly anaerobic species. Nevertheless, among usual bacterial pathogens, the development of axenic media for the culture of Treponema pallidum or Mycobacterium leprae remains an important challenge that the patience and innovations of cultivators will enable them to overcome.

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New insights into the emerging role of oral spirochaetes in periodontal disease

Cited by 65 publications

References 149 publications

Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities

Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities

Inactivation of Cyclic Di-GMP Binding Protein TDE0214 Affects the Motility, Biofilm Formation, and Virulence of Treponema denticola

Current and Past Strategies for Bacterial Culture in Clinical Microbiology

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