New metabolites produced by endophyte Clonostachys rosea B5 − 2

Abstract

An endophyte fungus, Clonostachys rosea, which was isolated from mangrove plants, was subjected to a one-strain-many compounds (OSMAC) strategy. We have shown that modification of culture media enhanced the productions of secondary metabolites by C. rosea. It was found to produce four new compounds and one known compound, (-)-vertinolide (1), in a medium of rice with commercially apple juice.Furthermore, (-)-dihydrovertinolide (2) was phytotoxic action in lettuce seeding at a concentration of 50 mg L -1 .

Metabolites from Clonostachys Fungi and Their Biological Activities

“…Both (-)-vertinolide (143) and (-)-dihydrovertinolide (144) were isolated from Clonostachys rosea B5-2. (-)-Dihydrovertinolide (144) displayed phytotoxic activity against lettuce seedlings at a concentration of 50 μg/mL [58].…”

“…Five fatty acids named clonostach acids A (219), B (220), and C (221) were isolated from the endophytic fungus Clonostachys rosea B5-2 [58].…”

“…Fermentation of the Clonostachys rosea on white beans instead of rice afforded one γ-lactam clonostalactam (62) and two γ-lactones 3,5-dihydroxyfuran-2(5H)-one (141) and sapinofuranone B (142) that were not detected in the former extracts [11]. The apple juice supplemented solid rice media led to significant changes in the secondary metabolism of the fungus, Clonostachys rosea B5-2, and induced the production of four new compounds, (-)-dihydrovertinolide (144), clonostach acids A (219), B (220) and C (221) together with the known compound, (-)-vertinolide (143) [58]. Co-cultivation of the Bionectria sp.…”

Biological Activities of Some New Secondary Metabolites Isolated from Endophytic Fungi: A Review Study

“…Beyma) Zare & W. Gams PSU-H168 and PSU-H261 resulted in the isolation of three new compounds including two depsidones, simplicildones J and K 429 – 430 ( Figure 12 ) and one dihydroxanthenone derivative, globosuxanthone E 431 ( Figure 12 ) [ 96 ]. The apple juice supplemented solid rice media led to significant changes in the secondary metabolism of the endophytic fungus, Clonostachys rosea B5-2, and induced the production of four new compounds, (−)-dihydrovertinolide 432 ( Figure 12 ), and clonostach acids A 433 ( Figure 12 ), B 434 ( Figure 12 ), and C 435 ( Figure 12 ) [ 140 ]. Six new nonadride derivatives 436 – 441 ( Figure 12 ) were isolated from the endophytic fungus Talaromyces purpurogenus obtained from fresh leaves of the toxic medicinal plant Tylophora ovate [ 87 ].…”

“…Phtotoxicity assay against lettuce seedlings of compound 432 was carried out using a published protocol. The new compound (−)-dihydrovertinolide 432 exhibited phytotoxicity against lettuce seedlings at a concentration of 50 mg/L [ 140 ].…”